Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation life...Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power density.Using a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination.LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes.LECs’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be achieved.By replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses.Moreover,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.展开更多
In the present paper,the attention is focused on the characteristics of lightweight materials collection in the duct using acoustic resonance phenomena.The acoustic resonance was excited by using a controlled speaker ...In the present paper,the attention is focused on the characteristics of lightweight materials collection in the duct using acoustic resonance phenomena.The acoustic resonance was excited by using a controlled speaker at the middle of a test duct.We measured the sound pressure level,frequency response characteristics,acoustic damping ratio,mode shape,and lightweight materials response to acoustic resonance excited by a speaker.As a result,the acoustic damping ratio decreased as the mode number of acoustic resonance increased.The tissue strips and the lightweight materials were collected at the node of acoustic pressure when the acoustic resonance was excited.It was made clear that it is possible to control lightweight materials using acoustic resonance excited by a speaker.展开更多
基金the Shenzhen Science and Technology Program(KQTD20170810110913065 and 20200925174735005).
文摘Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power density.Using a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination.LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes.LECs’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be achieved.By replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses.Moreover,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.
基金supported by a research grant from Harada commemorative foundation
文摘In the present paper,the attention is focused on the characteristics of lightweight materials collection in the duct using acoustic resonance phenomena.The acoustic resonance was excited by using a controlled speaker at the middle of a test duct.We measured the sound pressure level,frequency response characteristics,acoustic damping ratio,mode shape,and lightweight materials response to acoustic resonance excited by a speaker.As a result,the acoustic damping ratio decreased as the mode number of acoustic resonance increased.The tissue strips and the lightweight materials were collected at the node of acoustic pressure when the acoustic resonance was excited.It was made clear that it is possible to control lightweight materials using acoustic resonance excited by a speaker.
