以鲜食葡萄“玫瑰香”为试验材料,研究3,6,8 m L/kg 3个浓度下,乙醇处理对果实采后贮藏品质及相关酶活性的影响。结果表明:3 m L/kg和6 m L/kg的乙醇处理均可不同程度延缓果实采后衰老,保持贮藏品质,但8 m L/kg的乙醇处理加速了果实衰老...以鲜食葡萄“玫瑰香”为试验材料,研究3,6,8 m L/kg 3个浓度下,乙醇处理对果实采后贮藏品质及相关酶活性的影响。结果表明:3 m L/kg和6 m L/kg的乙醇处理均可不同程度延缓果实采后衰老,保持贮藏品质,但8 m L/kg的乙醇处理加速了果实衰老。3 m L/kg和6 m L/kg的乙醇处理均能有效抑制果实质量损失和硬度下降(P<0. 05),延缓可溶性固形物升高和可滴定酸含量的下降(P<0. 05),抑制多聚半乳糖醛酸酶(PG)和过氧化物酶(POD)活性的升高(P<0. 05),并且3 m L/kg的乙醇处理效果优于6 m L/kg的乙醇处理;8 m L/kg的乙醇处理效果则与此相反。综上,适当的低浓度乙醇处理可延缓鲜食葡萄“玫瑰香”的采后衰老,有利于降低果实质量损失,抑制果实软化及相关酶活性的升高。展开更多
Complex I （the NADH：ubiquinone oxidoreductase） of the mitochondrial respiratory chain is a complicated, multi-subunit, membrane- bound assembly and contains more than 40 different proteins in higher plants. In this...Complex I （the NADH：ubiquinone oxidoreductase） of the mitochondrial respiratory chain is a complicated, multi-subunit, membrane- bound assembly and contains more than 40 different proteins in higher plants. In this paper, we characterize the Arabidopsis homologue （designated as AtCIB22） of the B22 subunit of eukaryotic mitochondriai Complex I. AtCIB22 is a single-copy gene and is highly con- served throughout eukaryotes. AtCIB22 protein is located in mitochondria and the AtC1B22 gene is widely expressed in different tissues. Mutant Arabidopsis plants with a disrupted AtC1B22 gene display pleiotropic phenotypes including shorter roots, smaller plants and de- layed flowering. Stress analysis indicates that the AtC1B22 mutants＇ seed germination and early seedling growth are severely inhibited by sucrose deprivation stress but more tolerant to ethanol stress. Molecular analysis reveals that in moderate knockdown AtCIB22 mutants, genes including cell redox proteins and stress related proteins are significantly up-regulated, and that in severe knockdown AtCIB22 mu- tants, the alternative respiratory pathways including NDA1, NDB2, AOXla and AtPUMP1 are remarkably elevated. These data demon- strate that AtCIB22 is essential for plant development and mitochondrial electron transport chains in Arabidopsis. Our findings also en- hance our understanding about the physiological role of Complex I in plants.展开更多
Agricultural residues are important renewable biomass resources that have not received much research attention. Ginger stalk is a major agricultural waste in China.The extraction of cellulose from ginger stalk would c...Agricultural residues are important renewable biomass resources that have not received much research attention. Ginger stalk is a major agricultural waste in China.The extraction of cellulose from ginger stalk would convert this waste into a high value-added product and, simultaneously, contribute to environmental protection. This research studied the characteristics of cellulose extracted from ginger stalk by two different treatments:(i) potassium hydroxide(KOH) treatment and(ii) nitric acid-ethanol(NAE) treatment. The optimal condition for the KOH treatment was obtained, it was at 1∶30 solidto-liquid ratio(SLR) for 5 h extraction time with 14 wt% KOH. The optimal condition for the NAE treatment was as follows: 1∶40 SLR, 4 h extraction time, and a reaction temperature of 90℃. However, the cellulose obtained by NAE treatment was severely degraded than that by KOH treatment. The Fourier-transform infrared(FT-IR) spectroscopy analyses revealed that both treatments successfully dissolved the lignin and hemicellulose. Two treatments showed a higher cellulose yield, and the extracted cellulose had more crystal structure.展开更多
文摘以鲜食葡萄“玫瑰香”为试验材料,研究3,6,8 m L/kg 3个浓度下,乙醇处理对果实采后贮藏品质及相关酶活性的影响。结果表明:3 m L/kg和6 m L/kg的乙醇处理均可不同程度延缓果实采后衰老,保持贮藏品质,但8 m L/kg的乙醇处理加速了果实衰老。3 m L/kg和6 m L/kg的乙醇处理均能有效抑制果实质量损失和硬度下降(P<0. 05),延缓可溶性固形物升高和可滴定酸含量的下降(P<0. 05),抑制多聚半乳糖醛酸酶(PG)和过氧化物酶(POD)活性的升高(P<0. 05),并且3 m L/kg的乙醇处理效果优于6 m L/kg的乙醇处理;8 m L/kg的乙醇处理效果则与此相反。综上,适当的低浓度乙醇处理可延缓鲜食葡萄“玫瑰香”的采后衰老,有利于降低果实质量损失,抑制果实软化及相关酶活性的升高。
基金supported by the National Basic Research Program of China (No. 2009CB941503)
文摘Complex I （the NADH：ubiquinone oxidoreductase） of the mitochondrial respiratory chain is a complicated, multi-subunit, membrane- bound assembly and contains more than 40 different proteins in higher plants. In this paper, we characterize the Arabidopsis homologue （designated as AtCIB22） of the B22 subunit of eukaryotic mitochondriai Complex I. AtCIB22 is a single-copy gene and is highly con- served throughout eukaryotes. AtCIB22 protein is located in mitochondria and the AtC1B22 gene is widely expressed in different tissues. Mutant Arabidopsis plants with a disrupted AtC1B22 gene display pleiotropic phenotypes including shorter roots, smaller plants and de- layed flowering. Stress analysis indicates that the AtC1B22 mutants＇ seed germination and early seedling growth are severely inhibited by sucrose deprivation stress but more tolerant to ethanol stress. Molecular analysis reveals that in moderate knockdown AtCIB22 mutants, genes including cell redox proteins and stress related proteins are significantly up-regulated, and that in severe knockdown AtCIB22 mu- tants, the alternative respiratory pathways including NDA1, NDB2, AOXla and AtPUMP1 are remarkably elevated. These data demon- strate that AtCIB22 is essential for plant development and mitochondrial electron transport chains in Arabidopsis. Our findings also en- hance our understanding about the physiological role of Complex I in plants.
基金financial support from the National Basic Research Program of China ( Grant No.2014CB460610 )National Science Foundation of China (Grant Nos.2157061214 and 21406126)Department of Education of Shandong Province (Grant No.J14LC11)
文摘Agricultural residues are important renewable biomass resources that have not received much research attention. Ginger stalk is a major agricultural waste in China.The extraction of cellulose from ginger stalk would convert this waste into a high value-added product and, simultaneously, contribute to environmental protection. This research studied the characteristics of cellulose extracted from ginger stalk by two different treatments:(i) potassium hydroxide(KOH) treatment and(ii) nitric acid-ethanol(NAE) treatment. The optimal condition for the KOH treatment was obtained, it was at 1∶30 solidto-liquid ratio(SLR) for 5 h extraction time with 14 wt% KOH. The optimal condition for the NAE treatment was as follows: 1∶40 SLR, 4 h extraction time, and a reaction temperature of 90℃. However, the cellulose obtained by NAE treatment was severely degraded than that by KOH treatment. The Fourier-transform infrared(FT-IR) spectroscopy analyses revealed that both treatments successfully dissolved the lignin and hemicellulose. Two treatments showed a higher cellulose yield, and the extracted cellulose had more crystal structure.
