Dimeric estradiol enzyme model (2) was synthesized by etherification of 2,4-bis(N-imidazolylmethyl)-17β-estradiol (1) with 1,3-dibromopropane in the presence of anhydrous K2CO3. Hydrolysis of carboxylates and phospha...Dimeric estradiol enzyme model (2) was synthesized by etherification of 2,4-bis(N-imidazolylmethyl)-17β-estradiol (1) with 1,3-dibromopropane in the presence of anhydrous K2CO3. Hydrolysis of carboxylates and phosphates catalyzed by the model showed Michaelis-Menten kinetic behavior. Hydrophobic interaction between the model and ester accelerates the hydrolysis markedly, rate enhancement of up to 65 and 285 fold, relative to imidazole, is observed.展开更多
基金Project(No. 129402012 and 29632004)supported by the National Natural Science FouDdation of China and the Special Fupds of the State Educational Committee for Doctorate Scientific Research of China
文摘Dimeric estradiol enzyme model (2) was synthesized by etherification of 2,4-bis(N-imidazolylmethyl)-17β-estradiol (1) with 1,3-dibromopropane in the presence of anhydrous K2CO3. Hydrolysis of carboxylates and phosphates catalyzed by the model showed Michaelis-Menten kinetic behavior. Hydrophobic interaction between the model and ester accelerates the hydrolysis markedly, rate enhancement of up to 65 and 285 fold, relative to imidazole, is observed.
