To investigate the effects of ECzhi Tiangui Granule （二至天癸颗粒, ETG） on sequential expressions of integrin β 3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation （COH...To investigate the effects of ECzhi Tiangui Granule （二至天癸颗粒, ETG） on sequential expressions of integrin β 3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation （COH） and implantation period. Methods： Seventy-five Mature female Kunming mice were randomly divided into 3 groups, a normal control group, a model group, and a treatment group administrated with ETG for 10 days, 25 in each group. After mated with male mice, every 5 mice were sacrified in each group at the 0, 2nd, 4th, 6th, and 8th days to take their endometrium. In-situ hybridization was used to detect the expressions of integrin β 3 and osteopontin in the endometrium. Results： mRNA expressions of integrin β 3 and osteopontin in the endometrium during implantation period showed similar time sequence rules in the treatment group to those in the normal control group; the peak values of them were a little lower in the treatment group than the normal control without significant differences. In the model group, integrin β 3 mRNA expression was higher at the 2nd day, obviously lower at the 4th and 6th days, and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th, 6th, and 8th days, compared with the normal control and the treatment groups （P〈0.05, P〈0.01）. Conclusions： COH might influence the sequential expressions of integrin β 3 and its ligand osteopontin, bring forward the integdn β 3 expression peak, impact on the cooperation of integrin β 3 and osteopontin, so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrin β 3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.展开更多
目的:探讨补肾养血方药对气态甲醛慢性染毒小鼠子宫内膜容受性的影响。方法:将30只性成熟SPF级雌性昆明种小鼠随机分为空白对照组、染毒组和中药治疗组,每组10只,空白对照组给予吸入经过滤的新鲜空气,染毒组和中药治疗组给予1.0 mg/m3...目的:探讨补肾养血方药对气态甲醛慢性染毒小鼠子宫内膜容受性的影响。方法:将30只性成熟SPF级雌性昆明种小鼠随机分为空白对照组、染毒组和中药治疗组,每组10只,空白对照组给予吸入经过滤的新鲜空气,染毒组和中药治疗组给予1.0 mg/m3浓度气态甲醛动态吸入式染毒,6 h/d,同时中药治疗组每日予补肾养血中药灌胃,空白对照组和染毒组予等量生理盐水灌胃,连续14 d;将小鼠雌雄(2:1)合笼处理,于孕5 d处死小鼠,取出子宫,计算子宫脏器系数,观察胚胎着床情况;用RT-PCR和Real-time PCR法检测各组小鼠子宫Hoxa10、Hoxa11 m RNA的表达。结果:染毒组小鼠子宫脏器系数、子宫Hoxa10、Hoxa11 m RNA表达均低于空白对照组,差异均有统计学意义(P<0.05或P<0.01),胚胎着床数低于空白对照组,但差异无统计学意义(P>0.05)。中药治疗组小鼠子宫Hoxa10、Hoxa11 m RNA表达高于染毒组,差异均有统计学意义(P<0.05或P<0.01)。结论:补肾养血方药能使气态甲醛慢性染毒小鼠子宫Hoxa10、Hoxa11 m RNA表达升高,从而改善子宫内膜容受性。展开更多
基金Supported by the National Natural Science Foundation of China (No.30901920)Shandong Province Natural Funded Projects(No. Q2008C17)Shandong Province Traditional Chinese Medical Science and Technology Development Programs(No.2009-038)
文摘To investigate the effects of ECzhi Tiangui Granule （二至天癸颗粒, ETG） on sequential expressions of integrin β 3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation （COH） and implantation period. Methods： Seventy-five Mature female Kunming mice were randomly divided into 3 groups, a normal control group, a model group, and a treatment group administrated with ETG for 10 days, 25 in each group. After mated with male mice, every 5 mice were sacrified in each group at the 0, 2nd, 4th, 6th, and 8th days to take their endometrium. In-situ hybridization was used to detect the expressions of integrin β 3 and osteopontin in the endometrium. Results： mRNA expressions of integrin β 3 and osteopontin in the endometrium during implantation period showed similar time sequence rules in the treatment group to those in the normal control group; the peak values of them were a little lower in the treatment group than the normal control without significant differences. In the model group, integrin β 3 mRNA expression was higher at the 2nd day, obviously lower at the 4th and 6th days, and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th, 6th, and 8th days, compared with the normal control and the treatment groups （P〈0.05, P〈0.01）. Conclusions： COH might influence the sequential expressions of integrin β 3 and its ligand osteopontin, bring forward the integdn β 3 expression peak, impact on the cooperation of integrin β 3 and osteopontin, so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrin β 3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.
文摘目的:探讨补肾养血方药对气态甲醛慢性染毒小鼠子宫内膜容受性的影响。方法:将30只性成熟SPF级雌性昆明种小鼠随机分为空白对照组、染毒组和中药治疗组,每组10只,空白对照组给予吸入经过滤的新鲜空气,染毒组和中药治疗组给予1.0 mg/m3浓度气态甲醛动态吸入式染毒,6 h/d,同时中药治疗组每日予补肾养血中药灌胃,空白对照组和染毒组予等量生理盐水灌胃,连续14 d;将小鼠雌雄(2:1)合笼处理,于孕5 d处死小鼠,取出子宫,计算子宫脏器系数,观察胚胎着床情况;用RT-PCR和Real-time PCR法检测各组小鼠子宫Hoxa10、Hoxa11 m RNA的表达。结果:染毒组小鼠子宫脏器系数、子宫Hoxa10、Hoxa11 m RNA表达均低于空白对照组,差异均有统计学意义(P<0.05或P<0.01),胚胎着床数低于空白对照组,但差异无统计学意义(P>0.05)。中药治疗组小鼠子宫Hoxa10、Hoxa11 m RNA表达高于染毒组,差异均有统计学意义(P<0.05或P<0.01)。结论:补肾养血方药能使气态甲醛慢性染毒小鼠子宫Hoxa10、Hoxa11 m RNA表达升高,从而改善子宫内膜容受性。
