目的: 探讨地塞米松(dexam ethasone,D EX)对培养的兔角膜上皮细胞和角膜上皮伤口愈合的影响。方法: 体外实验原代培养兔角膜上皮细胞, 用四唑盐(M TT)实验和流式细胞仪检测不同浓度 D EX 对兔角膜上皮细胞的作用, 同时通过 H E 、免疫...目的: 探讨地塞米松(dexam ethasone,D EX)对培养的兔角膜上皮细胞和角膜上皮伤口愈合的影响。方法: 体外实验原代培养兔角膜上皮细胞, 用四唑盐(M TT)实验和流式细胞仪检测不同浓度 D EX 对兔角膜上皮细胞的作用, 同时通过 H E 、免疫组织化学染色和扫描电镜及临床观察兔角膜上皮细胞刮除后 0.1g/L D EX 用药组和对照组伤口愈合的情况。结果: M TT 检测显示小于 0.1g/L 浓度的 D EX 对兔角膜上皮细胞的生长无明显影响。流式细胞仪检测到 0.1g/L 浓度的 D EX 对兔角膜细胞生长周期无抑制作用。动物实验: 0.1g/L 地塞米松作用后上皮愈合时间显著性缩短, 两组中新生成的上皮细胞均有较强的 PC N A 蛋白的表达。0.1g/L 地塞米松组与对照组相比, 电子显微镜检查角膜基质排列更规则, 炎症反应较轻。结论: 低于 0.1g/L 浓度的地塞米松对培养的兔角膜上皮细胞的生长无抑制作用。0.1g/L 浓度的地塞米松眼液能有效的促进角膜上皮生长并降低炎症反应, 将有利于准分子激光角膜屈光手术后早期角膜伤口愈合。展开更多
Chrysophanol （1,8-dihydroxy-3-methylanthraquinone） is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chrysophanol has been reported to have both protective and curative activity...Chrysophanol （1,8-dihydroxy-3-methylanthraquinone） is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chrysophanol has been reported to have both protective and curative activity against Sphaerotheca fuliginea （Schlechtend.：Fr.） Pollacci, the causal agent of cucumber powdery mildew. In this paper the ultrastructure of powdery mildew on cucumber leaves was studied using electron microscopy after the leaves were treated with chrysophanol. Results showed that preventive treatments with chrysophanol affected fungal development, including spore germination, appressorial formation, and penetration. In the curative treatment, chrysophanol affected fungal survival, resulting in broken cell wall of germ tubes, swelling and collapse of hyphal tips, hyphal malformation, delayed and reduced sporulation. The morphological changes induced by chrysophanol at the ultrastructural level were reflected by haustorium deformation, vacuolization, abortion, and necrosis. Host cell walls infected or adjacent to haustoria were thickened. All these morphological changes of S. fuliginea further confirmed the fungicidal activity of chrysophanol on powdery mildew of cucumber.展开更多
In this paper,the results of exploration macrostructure and microstructure of continuous cast copper bars are shown. Quantitative parameters of copper cast bars depending on the speed of continuous casting are rated. ...In this paper,the results of exploration macrostructure and microstructure of continuous cast copper bars are shown. Quantitative parameters of copper cast bars depending on the speed of continuous casting are rated. It is fixed that independent of speed of casting the macrocrystalline columnar structure consisting of four zones is formed. By means of raster electron microscopy,the microstructure of cast copper is studied. It is determined that the accumulations of eutectic Cu-Cu2O in the form of lines and gas pores by size from 2 to 35 microns at the boundaries grain are observed. With the use of the transmission electron microscope,specific dislocation configuration on cast copper sub-boundary grains is determined. It indicates to high-temperature strain of cupper in the course of cast bar solidification. The important finding can be used for the design technology of copper continuous casting and plastic working of cast bars.展开更多
文摘目的: 探讨地塞米松(dexam ethasone,D EX)对培养的兔角膜上皮细胞和角膜上皮伤口愈合的影响。方法: 体外实验原代培养兔角膜上皮细胞, 用四唑盐(M TT)实验和流式细胞仪检测不同浓度 D EX 对兔角膜上皮细胞的作用, 同时通过 H E 、免疫组织化学染色和扫描电镜及临床观察兔角膜上皮细胞刮除后 0.1g/L D EX 用药组和对照组伤口愈合的情况。结果: M TT 检测显示小于 0.1g/L 浓度的 D EX 对兔角膜上皮细胞的生长无明显影响。流式细胞仪检测到 0.1g/L 浓度的 D EX 对兔角膜细胞生长周期无抑制作用。动物实验: 0.1g/L 地塞米松作用后上皮愈合时间显著性缩短, 两组中新生成的上皮细胞均有较强的 PC N A 蛋白的表达。0.1g/L 地塞米松组与对照组相比, 电子显微镜检查角膜基质排列更规则, 炎症反应较轻。结论: 低于 0.1g/L 浓度的地塞米松对培养的兔角膜上皮细胞的生长无抑制作用。0.1g/L 浓度的地塞米松眼液能有效的促进角膜上皮生长并降低炎症反应, 将有利于准分子激光角膜屈光手术后早期角膜伤口愈合。
基金supported by the Special Fund for Agroscientific Research in the Public Interest, Ministry of Agriculture of China (200903004)
文摘Chrysophanol （1,8-dihydroxy-3-methylanthraquinone） is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chrysophanol has been reported to have both protective and curative activity against Sphaerotheca fuliginea （Schlechtend.：Fr.） Pollacci, the causal agent of cucumber powdery mildew. In this paper the ultrastructure of powdery mildew on cucumber leaves was studied using electron microscopy after the leaves were treated with chrysophanol. Results showed that preventive treatments with chrysophanol affected fungal development, including spore germination, appressorial formation, and penetration. In the curative treatment, chrysophanol affected fungal survival, resulting in broken cell wall of germ tubes, swelling and collapse of hyphal tips, hyphal malformation, delayed and reduced sporulation. The morphological changes induced by chrysophanol at the ultrastructural level were reflected by haustorium deformation, vacuolization, abortion, and necrosis. Host cell walls infected or adjacent to haustoria were thickened. All these morphological changes of S. fuliginea further confirmed the fungicidal activity of chrysophanol on powdery mildew of cucumber.
文摘In this paper,the results of exploration macrostructure and microstructure of continuous cast copper bars are shown. Quantitative parameters of copper cast bars depending on the speed of continuous casting are rated. It is fixed that independent of speed of casting the macrocrystalline columnar structure consisting of four zones is formed. By means of raster electron microscopy,the microstructure of cast copper is studied. It is determined that the accumulations of eutectic Cu-Cu2O in the form of lines and gas pores by size from 2 to 35 microns at the boundaries grain are observed. With the use of the transmission electron microscope,specific dislocation configuration on cast copper sub-boundary grains is determined. It indicates to high-temperature strain of cupper in the course of cast bar solidification. The important finding can be used for the design technology of copper continuous casting and plastic working of cast bars.
