Objective: To study the impacts of exposure to electromagnetic radiation(EMR) on liver function in rats. Methods: Twenty adult male Sprague-Dawley rats were randomly divided into normal group and radiated group. The r...Objective: To study the impacts of exposure to electromagnetic radiation(EMR) on liver function in rats. Methods: Twenty adult male Sprague-Dawley rats were randomly divided into normal group and radiated group. The rats in normal group were not radiated, those in radiated group were exposed to EMR 4 h/d for 18 consecutive days. Rats were sacrificed immediately after the end of the experiment. The serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST), and those of malondialdehyde(MDA) and glutathione(GSH) in liver tissue were evaluated by colorimetric method. The liver histopathological changes were observed by hematoxylin and eosin staining and the protein expression of bax and bcl-2 in liver tissue were detected by immunohistochemical method. Terminal-deoxynucleotidyl transferase mediated nick and labelling(TUNEL) method was used for analysis of apoptosis in liver. Results: Compared with the normal rats, the serum levels of ALT and AST in the radiated group had no obvious changes(P>0.05), while the contents of MDA increased(P<0.01) and those of GSH decreased(P<0.01) in liver tissues. The histopathology examination showed diffuse hepatocyte swelling and vacuolation, small pieces and focal necrosis. The immunohistochemical results displayed that the expression of the bax protein was higher and that of bcl-2 protein was lower in radiated group. The hepatocyte apoptosis rates in radiated group was higher than that in normal group(all P<0.01). Conclusion: The exposure to 900 MHz mobile phone 4 h/d for 18 days could induce the liver histological changes, which may be partly due to the apoptosis and oxidative stress induced in liver tissue by electromagnetic radiation.展开更多
Based on electromagnetic radiation characteristics, the present research studied the damage evolution of rock under uniaxial compression. Besides, this research built the coal-rock damage evolution model considered re...Based on electromagnetic radiation characteristics, the present research studied the damage evolution of rock under uniaxial compression. Besides, this research built the coal-rock damage evolution model considered residual strength. The applicability and accuracy of the model were verified through experiments. The results show that coal-rock damage evolution consists of four periods. The first period is from the beginning of compression to nearly 20% of the stress peak value, during which the damage variable changes stably about 0.1, and accordingly a few of electromagnetic radiation signals emerge. The second period is from about 20% to 70% of the stress peak value. The damage has stable development, and the parameter of electromagnetic radiation characteristics turns larger continuously with the increase of stress. The third period is when the damage has accelerated development, the coal-rock was broken which result from sharp increasing of the damage variable, meanwhile a great quantity of electromagnetic radiation signals emerge. The fourth period is after the coal-rock fracture, during which the damage variable corresponding to the parameter of electromagnetic radiation characteristics has a stable development. This research has great academic and realistic significance for further studies the electromagnetic radiation characteristics of coal-rock under loading and damage and the forecasting of coal-rock dynamic disasters.展开更多
基金supported by the Natural Science Foundation of Hebei Province(201220123)Youth Fund Project of He Bei University of Chinese Medicine(QNZ2014015)
文摘Objective: To study the impacts of exposure to electromagnetic radiation(EMR) on liver function in rats. Methods: Twenty adult male Sprague-Dawley rats were randomly divided into normal group and radiated group. The rats in normal group were not radiated, those in radiated group were exposed to EMR 4 h/d for 18 consecutive days. Rats were sacrificed immediately after the end of the experiment. The serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST), and those of malondialdehyde(MDA) and glutathione(GSH) in liver tissue were evaluated by colorimetric method. The liver histopathological changes were observed by hematoxylin and eosin staining and the protein expression of bax and bcl-2 in liver tissue were detected by immunohistochemical method. Terminal-deoxynucleotidyl transferase mediated nick and labelling(TUNEL) method was used for analysis of apoptosis in liver. Results: Compared with the normal rats, the serum levels of ALT and AST in the radiated group had no obvious changes(P>0.05), while the contents of MDA increased(P<0.01) and those of GSH decreased(P<0.01) in liver tissues. The histopathology examination showed diffuse hepatocyte swelling and vacuolation, small pieces and focal necrosis. The immunohistochemical results displayed that the expression of the bax protein was higher and that of bcl-2 protein was lower in radiated group. The hepatocyte apoptosis rates in radiated group was higher than that in normal group(all P<0.01). Conclusion: The exposure to 900 MHz mobile phone 4 h/d for 18 days could induce the liver histological changes, which may be partly due to the apoptosis and oxidative stress induced in liver tissue by electromagnetic radiation.
基金provided by the National Key Technology R&D Program of China (No. 2012BAK09B01)the National Natural Science Foundation of China (Nos. 50904067 and 51104156)+1 种基金the Program for the New Century Excellent Talents in University of China (No. NCET-10-0768)the Jiangsu Planned Projects for Postdoctoral Research Funds of China (No. 1102085C)
文摘Based on electromagnetic radiation characteristics, the present research studied the damage evolution of rock under uniaxial compression. Besides, this research built the coal-rock damage evolution model considered residual strength. The applicability and accuracy of the model were verified through experiments. The results show that coal-rock damage evolution consists of four periods. The first period is from the beginning of compression to nearly 20% of the stress peak value, during which the damage variable changes stably about 0.1, and accordingly a few of electromagnetic radiation signals emerge. The second period is from about 20% to 70% of the stress peak value. The damage has stable development, and the parameter of electromagnetic radiation characteristics turns larger continuously with the increase of stress. The third period is when the damage has accelerated development, the coal-rock was broken which result from sharp increasing of the damage variable, meanwhile a great quantity of electromagnetic radiation signals emerge. The fourth period is after the coal-rock fracture, during which the damage variable corresponding to the parameter of electromagnetic radiation characteristics has a stable development. This research has great academic and realistic significance for further studies the electromagnetic radiation characteristics of coal-rock under loading and damage and the forecasting of coal-rock dynamic disasters.
