One new ecdysteroid, (24 R ) 24 (2 hydroxyethyl) 20 hydroxyecdysone (3), as well as three known ecdysteroids, has been isolated from Chinese herb Serratula strangulata and these compounds 1-4 showed effecti...One new ecdysteroid, (24 R ) 24 (2 hydroxyethyl) 20 hydroxyecdysone (3), as well as three known ecdysteroids, has been isolated from Chinese herb Serratula strangulata and these compounds 1-4 showed effective antioxidant activity on AAPH induced hemolysis of human RBC and Fe 2+ + cysteine induced lipid peroxidation of liver microsome.展开更多
This paper describes the embryonic development of the common cutworm Spodoptera litura （Fabricius） （Lepidoptera： Noctuidae） which is divided into eight embryonic stages, through variations in the egg＇s contents....This paper describes the embryonic development of the common cutworm Spodoptera litura （Fabricius） （Lepidoptera： Noctuidae） which is divided into eight embryonic stages, through variations in the egg＇s contents. Experiments were conducted on the eggs ofS. litura under laboratory conditions. The highest concentration of the protein （min.-max.： 32-! 40 lag/egg） and the lowest of the carbohydrate （min.-max.： 3.0-44.0 lag/egg） were obtained; similarly, the DNA content was smaller （min.-max.： 1.0-38.2 lag/egg） than the RNA （min.-max.： 9.2-360.0 lag/egg） throughout the embryogenesis. Three peaks of ecdysteroid titre were observed which were responsible for appearance of particular events during embryogenesis. The last highest peak of 400_＋33.4 lag/egg was appeared at 84 h after oviposition when larva was ready to hatch. Therefore, it is concluded that eight embryonic stages in S. litura were characterized by fluctuation in the egg＇s contents, which were responsible for gradual-progressive embryonic development. The egg-larval development did not show a normal distribution of embryonic developmental-time.展开更多
Objective:To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus(Blume) de Laub.Methods:Column chromatography was used for isolation of compounds from plan...Objective:To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus(Blume) de Laub.Methods:Column chromatography was used for isolation of compounds from plant material.The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques,including HSQC,HMBC,NOE-difference experiments.The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia(AML) and OCI-AML cells.Results:Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography.Its chemical structure was identified as 20-hydroxyecdysone(20HE),a cholestane-type ecdysteroid,by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses.Our goal was to test the anti-proliferative activity of 20 HE using the OCI-AML cell line.20 HE significantly decreased OCI cell number at a concentration of 1 mg/mL,whereas lower concentrations were ineffective.Moreover,this decrease was due to partial blockage of the G_1/S phase of the cell cycle,with a reduction of cells in the G_2M phase,not due to increased apoptosis.Conclusions:This indicates that 20 HE significantly decreases the number of cells in the G_1/S phase of the cell cycle in human AML cells.This is the first time that the anti-proliferative activity of 20 HE against a human tumor cell line has been reported.展开更多
This paper describes effects of sublethal-doses of chlorfluazuron on morphology of embryogenesis when LD10 （1.00 ng/larva） or LD30 （3.75 ng/larva） was applied topically to newly-ecdysed fifth-instars of common cut...This paper describes effects of sublethal-doses of chlorfluazuron on morphology of embryogenesis when LD10 （1.00 ng/larva） or LD30 （3.75 ng/larva） was applied topically to newly-ecdysed fifth-instars of common cutworm, Spodoptera litura F.. Experiments were conducted on eggs of F1 generation laid by subsequent adults under laboratory conditions. Fecundity was significantly reduced by LD10 and more significantly reduced by LD30 compared with untreated females. Number of unfertilized-unhatched-eggs was not significantly affected by the treatments, Fertilized eggs revealed changes in external coloration of eggs. Embryonic development of S. litura was divided into eight embryonic stages. During stage 5 （12-16 h after oviposition）, in LD10-treated-egg, crescent-shaped-embryo was affectedly differentiated. During the same stage, it was seriously affected in LD30-treated-egg. During stage 7 （32-64 h after oviposition）, in LD10-treated-egg, organogenesis was established, but body structures were affectedly developed. In LD30-treated-egg, body structures were more affectedly developed during the same stage. It is concluded that embryonic developmental stages were affected by LD10 and more seriously affected by LD30. The effects of residualchlorfluazuron on embryogenesis are thought to be one of the factors responsible for the reduction in fertility and hatchability caused by sublethal doses of chlorfluazuron in S. litura.展开更多
文摘One new ecdysteroid, (24 R ) 24 (2 hydroxyethyl) 20 hydroxyecdysone (3), as well as three known ecdysteroids, has been isolated from Chinese herb Serratula strangulata and these compounds 1-4 showed effective antioxidant activity on AAPH induced hemolysis of human RBC and Fe 2+ + cysteine induced lipid peroxidation of liver microsome.
文摘This paper describes the embryonic development of the common cutworm Spodoptera litura （Fabricius） （Lepidoptera： Noctuidae） which is divided into eight embryonic stages, through variations in the egg＇s contents. Experiments were conducted on the eggs ofS. litura under laboratory conditions. The highest concentration of the protein （min.-max.： 32-! 40 lag/egg） and the lowest of the carbohydrate （min.-max.： 3.0-44.0 lag/egg） were obtained; similarly, the DNA content was smaller （min.-max.： 1.0-38.2 lag/egg） than the RNA （min.-max.： 9.2-360.0 lag/egg） throughout the embryogenesis. Three peaks of ecdysteroid titre were observed which were responsible for appearance of particular events during embryogenesis. The last highest peak of 400_＋33.4 lag/egg was appeared at 84 h after oviposition when larva was ready to hatch. Therefore, it is concluded that eight embryonic stages in S. litura were characterized by fluctuation in the egg＇s contents, which were responsible for gradual-progressive embryonic development. The egg-larval development did not show a normal distribution of embryonic developmental-time.
基金the National Foundation for Science & Technology Development(NAFOSTED) of Vietnam for financial support of this research(code:104.01-2013.62)the Italian Ministery of Foreign Affairs and International Cooperation(MAECI)
文摘Objective:To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus(Blume) de Laub.Methods:Column chromatography was used for isolation of compounds from plant material.The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques,including HSQC,HMBC,NOE-difference experiments.The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia(AML) and OCI-AML cells.Results:Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography.Its chemical structure was identified as 20-hydroxyecdysone(20HE),a cholestane-type ecdysteroid,by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses.Our goal was to test the anti-proliferative activity of 20 HE using the OCI-AML cell line.20 HE significantly decreased OCI cell number at a concentration of 1 mg/mL,whereas lower concentrations were ineffective.Moreover,this decrease was due to partial blockage of the G_1/S phase of the cell cycle,with a reduction of cells in the G_2M phase,not due to increased apoptosis.Conclusions:This indicates that 20 HE significantly decreases the number of cells in the G_1/S phase of the cell cycle in human AML cells.This is the first time that the anti-proliferative activity of 20 HE against a human tumor cell line has been reported.
文摘This paper describes effects of sublethal-doses of chlorfluazuron on morphology of embryogenesis when LD10 （1.00 ng/larva） or LD30 （3.75 ng/larva） was applied topically to newly-ecdysed fifth-instars of common cutworm, Spodoptera litura F.. Experiments were conducted on eggs of F1 generation laid by subsequent adults under laboratory conditions. Fecundity was significantly reduced by LD10 and more significantly reduced by LD30 compared with untreated females. Number of unfertilized-unhatched-eggs was not significantly affected by the treatments, Fertilized eggs revealed changes in external coloration of eggs. Embryonic development of S. litura was divided into eight embryonic stages. During stage 5 （12-16 h after oviposition）, in LD10-treated-egg, crescent-shaped-embryo was affectedly differentiated. During the same stage, it was seriously affected in LD30-treated-egg. During stage 7 （32-64 h after oviposition）, in LD10-treated-egg, organogenesis was established, but body structures were affectedly developed. In LD30-treated-egg, body structures were more affectedly developed during the same stage. It is concluded that embryonic developmental stages were affected by LD10 and more seriously affected by LD30. The effects of residualchlorfluazuron on embryogenesis are thought to be one of the factors responsible for the reduction in fertility and hatchability caused by sublethal doses of chlorfluazuron in S. litura.
