As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular ...As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under field conditions was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry（MALDI-TOF/TOF MS）. A total of 60 senescence-associated proteins were identified. The identified proteins are involved in many biological processes, especially energy, metabolism and protein synthesis. Several of the identified proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.展开更多
This study is aimed to explore the spectrum reflection characteristics of summer corn leaves in different fertilization conditions.Using hyperspectral remote sensing technology,the experiments were conducted in fields...This study is aimed to explore the spectrum reflection characteristics of summer corn leaves in different fertilization conditions.Using hyperspectral remote sensing technology,the experiments were conducted in fields to collect the hyperspectral images of Denghai 605( DH605) and Ludan 981( LD981) in different growth period under five fertilization treatments,and then the reflectance of corn ear leaves was extracted by ENVI software. The five fertilization treatments included the control( CK) with no fertilization,40 kg and 30 kg of controlled-release fertilizer per 666. 67 m2 as base( K40 and K30),50 kg and 40 kg of compound fertilizer per 666. 67 m2 as base with 15 kg urea as seed fertilizer( F50 + N and F40 + N). The reflectance spectrums of the two corn cultivars under different fertilization treatments showed the approximately same changing trend with a reflection peak at green band( 550 nm) and a higher reflection platform at near infrared band( 760 nm-1050 nm). At the heading to filling stage,the reflectance of DH605 and LD981 was the highest under the CK,followed by the K30 and F40 + N respectively. At the filling to dough stage,the reflectance of DH605 and LD981 was the highest under the treatment K30 and F40 + N respectively,which was obviously higher than that of the other treatments. In the conditions of compound fertilizer,except the late filling stage,LD981 had little higher reflectance than DH605 at the other stages. In the conditions of controlled-release fertilizer and at dough to mature stage,LD981 had obviously higher reflectance compared to the other stages,and also higher than that of DH605; there was not obvious difference in reflectance LD981 and DH605 at the other stages.展开更多
基金supports from the National Natural Science Foundation of China (31171497)the National Basic Research Program of China (973 Program, 2011CB100105)+1 种基金the Corn Industry Technology System, Ministry of Agriculture, China (CARS-02)the Special Fund for Agro-scientific Research in the Public Interest, China (201203096, 201203100)
文摘As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under field conditions was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry（MALDI-TOF/TOF MS）. A total of 60 senescence-associated proteins were identified. The identified proteins are involved in many biological processes, especially energy, metabolism and protein synthesis. Several of the identified proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.
基金Supported by Key Research and Development Program of Shandong Province(2016CYJS03A01-1)Key Technology Research of Precision Agriculture(CXGC2017B04)+1 种基金Key Application Technological Innovation Project of Agriculture in Shandong Province"Research and Application of Key Technologies for Early Rapid Identification of Wheat Diseases"Young Scholar Scientific Research Foundation of Shandong Academy of Agricultural Sciences"Study on Rapid Extraction Technology for County-wide Farmland Based on High Resolution Remote Sensing Image"(2015YQN47)
文摘This study is aimed to explore the spectrum reflection characteristics of summer corn leaves in different fertilization conditions.Using hyperspectral remote sensing technology,the experiments were conducted in fields to collect the hyperspectral images of Denghai 605( DH605) and Ludan 981( LD981) in different growth period under five fertilization treatments,and then the reflectance of corn ear leaves was extracted by ENVI software. The five fertilization treatments included the control( CK) with no fertilization,40 kg and 30 kg of controlled-release fertilizer per 666. 67 m2 as base( K40 and K30),50 kg and 40 kg of compound fertilizer per 666. 67 m2 as base with 15 kg urea as seed fertilizer( F50 + N and F40 + N). The reflectance spectrums of the two corn cultivars under different fertilization treatments showed the approximately same changing trend with a reflection peak at green band( 550 nm) and a higher reflection platform at near infrared band( 760 nm-1050 nm). At the heading to filling stage,the reflectance of DH605 and LD981 was the highest under the CK,followed by the K30 and F40 + N respectively. At the filling to dough stage,the reflectance of DH605 and LD981 was the highest under the treatment K30 and F40 + N respectively,which was obviously higher than that of the other treatments. In the conditions of compound fertilizer,except the late filling stage,LD981 had little higher reflectance than DH605 at the other stages. In the conditions of controlled-release fertilizer and at dough to mature stage,LD981 had obviously higher reflectance compared to the other stages,and also higher than that of DH605; there was not obvious difference in reflectance LD981 and DH605 at the other stages.
