Fluorinated smectic liquid crystals each with a biphenyl benzoate rigid core are investigated. Molecular structures of the studied compounds have difference only in fluorine position and the length of the carbon chain...Fluorinated smectic liquid crystals each with a biphenyl benzoate rigid core are investigated. Molecular structures of the studied compounds have difference only in fluorine position and the length of the carbon chain. Dielectric relaxation study and electro-optical measurements are carried out with the classical SSFLC geometry. The field-induced phase transitions are studied and the（E,T） phase diagram is established.展开更多
Here, we report a convenient and efficient synthesis strategy for the total synthesis of cyclic peptide reniochalistatin E and its conformational isomers with 32% overall yield. We found the linear peptide precursor w...Here, we report a convenient and efficient synthesis strategy for the total synthesis of cyclic peptide reniochalistatin E and its conformational isomers with 32% overall yield. We found the linear peptide precursor without side chain gave better cyclization yield.展开更多
Bacterial genomic DNA is highly organized into one or few compacted bodies known as nucleoid, which is composed of DNA, RNA and several DNA-binding proteins. These DNA-binding proteins require essential alterations in...Bacterial genomic DNA is highly organized into one or few compacted bodies known as nucleoid, which is composed of DNA, RNA and several DNA-binding proteins. These DNA-binding proteins require essential alterations in their expression during stationary phase of growth in order to re-spond to stressful environmental conditions. Dps (DNA-binding protein from starved cells) is one of such DNA-binding proteins, which accumulates most when E. coli cells reach to the stationary phase. Here, we have characterized Dps protein under various growth phases. Immunofluorescent microscopic observation reveals that Dps plays a key role in final round of genome compaction during the stationary phase. Similar results are also obtained by Western immunoblot analysis, after quantification of Dps protein from the exponential phase and early stationary phase nucleoid bound fractions, separated by sucrose density gradient centrifugation. Our results support the conclusion that Dps occupies more than half of the stationary phase nucleoid in E. coli.展开更多
The prevalence of functionalized nanoparticles in biological and clinical fields attracts intensive toxicology investigations.Minimizing the nanoparticles’ biohazard remains a challenge due to the insufficient unders...The prevalence of functionalized nanoparticles in biological and clinical fields attracts intensive toxicology investigations.Minimizing the nanoparticles’ biohazard remains a challenge due to the insufficient understanding on the nanoparticle-induced cell death mechanism.In the presented study,we observed the lysosome and genome injuries and so caused cell cycle changes and regulations of retinal ganglion neuron cell 5(RGC-5) induced by aminated and alkylated nanoparticles.Alkylated nanoparticles induced malignant lysosome and genome damages followed by severe post-self-repair responses.RGC-5 treated with alkylated nanoparticles presented dramatic S phase prolongation resulted from cyclin E accumulation mediated by Fbw7 downregulation,which assisted DNA replication after failed self-repair of the malignantly damaged DNA caused by alkylated nanoparticles.Differently,aminated nanoparticles in RGC-5 induced moderate lysosome and genome injuries and these damages could be repaired in the p21-involved pathway,so that cells did not induce apparent cyclin E accumulation nor Fbw7 downregulation as post-self-repair response.These results helped us to understand the toxicity of analogous nanoparticles on retinal ganglions such as glaucoma treatment.This work provides new insights into nanoparticle functionalization and toxicity in relation to the research on the toxicology and pathology of nerve cells.展开更多
Background&Objectives:The degree of liver injury in indeterminate chronic hepatitis B(CHB)infection patients with Hepatitis B e antigen(HBeAg)‐negative and persistently normal alanine aminotransferase(PNALT)level...Background&Objectives:The degree of liver injury in indeterminate chronic hepatitis B(CHB)infection patients with Hepatitis B e antigen(HBeAg)‐negative and persistently normal alanine aminotransferase(PNALT)levels is yet unclear.Therefore,we aimed to assess liver histological changes in such patients by liver biopsy and explore possible predictors.Methods:Overall,711 HBeAg‐negative CHB patients with PNALT levels who underwent liver biopsy from January 2017 to June 2022 were included in this retrospective study.The relationships between histological changes and predictors were assessed by smooth curve fitting and multivariate logistic regression analysis models.Data were also analyzed using American Association for the Study of Liver Disease(AASLD)modified alanine aminotransferase(ALT)criteria.Results:The proportion of significant histological changes in the indeterminate phase was higher than that in the inactive phase(53.97%vs.41.33%).The adjusted odds ratios(aORs)of significant necroinflammation and histological changes for the indeterminate phase were 2.05 and 1.43,respectively,when compared with the inactive phase by multivariate logistic regression analyses.Significant histological changes in the‐phase were positively associated with age,ALT,Aspartate aminotransferase(AST),Hepatitis B surface antigen(HBsAg),and Hepatitis B virus(HBV)DNA levels but negatively correlated with platelet(PLT)levels.HBV DNA≥5 log10 U/L and PLT<200�109/L were independent predictive factors for assessing histological changes in indeterminate‐phase patients.Similar analysis findings were obtained using the two sets of modified ALT criteria.Conclusions:Significant histological changes are not rare in indeterminatephase CHB patients and are higher than in the inactive phase,regardless of the ALT criteria.Histological investigation is strongly suggested for intermediate stage patients with HBV DNA>5 log10 U/L or PLT<200�109/L and antiviral therapy should be considered for such patients.展开更多
文摘Fluorinated smectic liquid crystals each with a biphenyl benzoate rigid core are investigated. Molecular structures of the studied compounds have difference only in fluorine position and the length of the carbon chain. Dielectric relaxation study and electro-optical measurements are carried out with the classical SSFLC geometry. The field-induced phase transitions are studied and the（E,T） phase diagram is established.
基金supported by the National Natural Science Foundation of China(No. 21372183)Program for Innovative Teams of Outstanding Young and Middle-aged Researchers in the Higher Education Institutions of Hubei Province(No. T201702)
文摘Here, we report a convenient and efficient synthesis strategy for the total synthesis of cyclic peptide reniochalistatin E and its conformational isomers with 32% overall yield. We found the linear peptide precursor without side chain gave better cyclization yield.
文摘Bacterial genomic DNA is highly organized into one or few compacted bodies known as nucleoid, which is composed of DNA, RNA and several DNA-binding proteins. These DNA-binding proteins require essential alterations in their expression during stationary phase of growth in order to re-spond to stressful environmental conditions. Dps (DNA-binding protein from starved cells) is one of such DNA-binding proteins, which accumulates most when E. coli cells reach to the stationary phase. Here, we have characterized Dps protein under various growth phases. Immunofluorescent microscopic observation reveals that Dps plays a key role in final round of genome compaction during the stationary phase. Similar results are also obtained by Western immunoblot analysis, after quantification of Dps protein from the exponential phase and early stationary phase nucleoid bound fractions, separated by sucrose density gradient centrifugation. Our results support the conclusion that Dps occupies more than half of the stationary phase nucleoid in E. coli.
基金supported by the National Natural Science Foundation of China (Nos.11204033,51773093)the Natural Science Foundation of Jiangsu Province (No.BK20141397)+3 种基金the Research Fund for the Doctoral Program of Higher Education of China (No.20120092120042)the CMA L’Oreal China Skin Grant 2015 (No.S2015121421)the Open Research Fund of State Key Laboratory of Natural Medicines,China Pharmaceutical University (No.SKLNMKF201803)Southeast University and Nanjing MedicalUniversity Cooperation Project (No.2242018K3DN14)
文摘The prevalence of functionalized nanoparticles in biological and clinical fields attracts intensive toxicology investigations.Minimizing the nanoparticles’ biohazard remains a challenge due to the insufficient understanding on the nanoparticle-induced cell death mechanism.In the presented study,we observed the lysosome and genome injuries and so caused cell cycle changes and regulations of retinal ganglion neuron cell 5(RGC-5) induced by aminated and alkylated nanoparticles.Alkylated nanoparticles induced malignant lysosome and genome damages followed by severe post-self-repair responses.RGC-5 treated with alkylated nanoparticles presented dramatic S phase prolongation resulted from cyclin E accumulation mediated by Fbw7 downregulation,which assisted DNA replication after failed self-repair of the malignantly damaged DNA caused by alkylated nanoparticles.Differently,aminated nanoparticles in RGC-5 induced moderate lysosome and genome injuries and these damages could be repaired in the p21-involved pathway,so that cells did not induce apparent cyclin E accumulation nor Fbw7 downregulation as post-self-repair response.These results helped us to understand the toxicity of analogous nanoparticles on retinal ganglions such as glaucoma treatment.This work provides new insights into nanoparticle functionalization and toxicity in relation to the research on the toxicology and pathology of nerve cells.
基金The Natural Science Foundation of Shandong Province,China,Grant/Award Number:ZR2019PH046the funds for the construction of key medical disciplines in Shenzhen,Grant/Award Number:SZXK076。
文摘Background&Objectives:The degree of liver injury in indeterminate chronic hepatitis B(CHB)infection patients with Hepatitis B e antigen(HBeAg)‐negative and persistently normal alanine aminotransferase(PNALT)levels is yet unclear.Therefore,we aimed to assess liver histological changes in such patients by liver biopsy and explore possible predictors.Methods:Overall,711 HBeAg‐negative CHB patients with PNALT levels who underwent liver biopsy from January 2017 to June 2022 were included in this retrospective study.The relationships between histological changes and predictors were assessed by smooth curve fitting and multivariate logistic regression analysis models.Data were also analyzed using American Association for the Study of Liver Disease(AASLD)modified alanine aminotransferase(ALT)criteria.Results:The proportion of significant histological changes in the indeterminate phase was higher than that in the inactive phase(53.97%vs.41.33%).The adjusted odds ratios(aORs)of significant necroinflammation and histological changes for the indeterminate phase were 2.05 and 1.43,respectively,when compared with the inactive phase by multivariate logistic regression analyses.Significant histological changes in the‐phase were positively associated with age,ALT,Aspartate aminotransferase(AST),Hepatitis B surface antigen(HBsAg),and Hepatitis B virus(HBV)DNA levels but negatively correlated with platelet(PLT)levels.HBV DNA≥5 log10 U/L and PLT<200�109/L were independent predictive factors for assessing histological changes in indeterminate‐phase patients.Similar analysis findings were obtained using the two sets of modified ALT criteria.Conclusions:Significant histological changes are not rare in indeterminatephase CHB patients and are higher than in the inactive phase,regardless of the ALT criteria.Histological investigation is strongly suggested for intermediate stage patients with HBV DNA>5 log10 U/L or PLT<200�109/L and antiviral therapy should be considered for such patients.
