AIM:To observe the analgesic effects of moxibustion in rats with chronic visceral hyperalgesia and its influence on the concentration of dynorphin(Dyn) and endomorphin(EM) in spinal cord.METHODS:The rat model of chron...AIM:To observe the analgesic effects of moxibustion in rats with chronic visceral hyperalgesia and its influence on the concentration of dynorphin(Dyn) and endomorphin(EM) in spinal cord.METHODS:The rat model of chronic visceral hyperalgesia was established by colorectal distention(CRD).In moxibustion(MX) group,moxibustion was applied once daily for 7 d;in sham moxibustion(SM) group,moxibustion was given to the same acupoints but with the nonsmoldered end of the moxa stick.Model control(MC) group and normal control group were also studied.The scoring system of abdominal withdrawal reflex was used to evaluate visceral pain for behavioral assessment.Enzyme linked immunosorbent assay was performed to determine the concentrations of Dyn and EM in spinal cord.RESULTS:Moxibustion significantly decreased visceral pain to CRD in this rat model,and no significant difference was detected between the SM group and the MC group.In MX group,moxibustion also increased the concentrations of Dyn and EM in spinal cord,and no significant difference was found between the SM group and the MC group.CONCLUSION:Moxibustion therapy can significantly enhance the pain threshold of rats with chronic visceral hyperalgesia,and the effect may be closely related to the increased concentration of Dyn and EM in spinal cord.展开更多
OBJECTIVE:To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin(POMC)and prodynorphin(PDYN)mRNA expressions and plasmaβ-endorphin(β-EP)content in rheumatoid arthritis(RA)ra...OBJECTIVE:To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin(POMC)and prodynorphin(PDYN)mRNA expressions and plasmaβ-endorphin(β-EP)content in rheumatoid arthritis(RA)rats,to understand the mechanism of moxibustion analgesia and its dose-effect relationship.METHODS:Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal control group.The RA model was created by raising rats in a windy(blowing with electric fan),cold(6℃±2℃),and wet(80%-90%humidity)environment for 20 days,12 h each day.This was followed byinjectionofFreund'scompleteadjuvant(0.15 mL)into the ankle.Then,rats were randomly divided into a model group,moxibustion groupⅠ,and moxibustion groupⅡ,with 12 rats in each group.In moxibustion groupsⅠandⅡ,moxibustion was given at Shenshu(BL 23)and Zusanli(ST 36)for 20and 40 min,respectively,once daily for 15 days.Hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content were determined.RESULTS:Compared with the normal group,the pressure pain threshold decreased,while the hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content increased in the moxibustion groups(P<0.01).Compared with the model group,the pressure pain threshold,hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content in the moxibustion groups increased significantly(P<0.01).Compared the moxibustion groupⅠ,the pain threshold,hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content in moxibustion groupⅡsignificantly increased(P<0.01).CONCLUSION:Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content inRArats.TheanalgesiceffectinmoxibustiongroupⅡisbetterthanthatinmoxibustiongroupⅠ.展开更多
It has been proved that endogenous opioids play an important pathophysiological role inthe lesions secondary to central nervous system trauma.Opiate antagonists and opioid antisera havebeen found to improve the outcom...It has been proved that endogenous opioids play an important pathophysiological role inthe lesions secondary to central nervous system trauma.Opiate antagonists and opioid antisera havebeen found to improve the outcome of experimental brain injury,Thyrotropin-releasing hormone(TRH),which appears to act in part as a functional antagonist of opioid system,has also beenproved to be effective in the treatment of experimental brain injury.The developments of these as-pects in our laboratory are reviewed.展开更多
Drug addiction is a chronic brain disorder characterized by withdrawal symptoms that occur during drug abstinence and a high tendency of relapse.Compared with the currently available pharmacological interventions,acup...Drug addiction is a chronic brain disorder characterized by withdrawal symptoms that occur during drug abstinence and a high tendency of relapse.Compared with the currently available pharmacological interventions,acupuncture therapy has the potential to help drug addicts stay away from drugs without major adverse side effects.It has taken decades of research to optimize the parameters of electrical acupoint stimulation for detoxification and for relapse prevention,as well as to establish a safe and easy procedure by which drug addicts can use it on themselves.The discovery that acupuncture can trigger the release of opioid substances from the brain in the 1970s provided the inspiration.Following this,basic research on animals made it possible to understand the mechanisms of action and establish the procedure for treating drug addictions.This article reviews the past,present,and foreseeable future regarding the use of acupuncture-related technique for the treatment of opiate addiction from the perspective of translational medicine.展开更多
目的研究强啡肽及P物质在尿毒症皮肤瘙痒中的相互作用。方法5／6肾大部切除模型（STNx），24周进入终末期肾功能衰竭（ESRD）。分为A：STNx＋P，B：STNx＋强，C：sTNx模型对照及D：假手术组。分别皮内注射P物质（SP）、强啡肽及生理盐...目的研究强啡肽及P物质在尿毒症皮肤瘙痒中的相互作用。方法5／6肾大部切除模型（STNx），24周进入终末期肾功能衰竭（ESRD）。分为A：STNx＋P，B：STNx＋强，C：sTNx模型对照及D：假手术组。分别皮内注射P物质（SP）、强啡肽及生理盐水。注射后30min内观察搔抓反应。ELISA法检测血中sP浓度，免疫组化法检测皮肤组织SP表达。结果5／6肾切除大鼠血中SP升高，皮内注射SP的大鼠进一步升高，而皮内注射强啡肽后可明显降低血中SP浓度（A-D：2530．0±236．3 vs 612．4±72．2 vs 1010．2±103．5 vs 240．2±36．5）。免疫组化皮肤组织SP的表达可见到相似的趋势。STNx＋P组大鼠搔抓次数最多，B组与C组差异无统计学意义（P〉0．05）。结论尿毒症大鼠皮肤瘙痒与其血中及皮肤组织中SP表达增高有关。皮内注射强啡肽不能诱发尿毒症大鼠皮肤瘙痒反应，并能降低其血中及皮肤组织中SP的水平。展开更多
基金Supported by The National Basic Research Program of China,973 Program,No 2009CB522900Scientific Research Grants of Shanghai Health Bureau,No 2009209Shanghai Leading Academic Discipline Project,No S30304
文摘AIM:To observe the analgesic effects of moxibustion in rats with chronic visceral hyperalgesia and its influence on the concentration of dynorphin(Dyn) and endomorphin(EM) in spinal cord.METHODS:The rat model of chronic visceral hyperalgesia was established by colorectal distention(CRD).In moxibustion(MX) group,moxibustion was applied once daily for 7 d;in sham moxibustion(SM) group,moxibustion was given to the same acupoints but with the nonsmoldered end of the moxa stick.Model control(MC) group and normal control group were also studied.The scoring system of abdominal withdrawal reflex was used to evaluate visceral pain for behavioral assessment.Enzyme linked immunosorbent assay was performed to determine the concentrations of Dyn and EM in spinal cord.RESULTS:Moxibustion significantly decreased visceral pain to CRD in this rat model,and no significant difference was detected between the SM group and the MC group.In MX group,moxibustion also increased the concentrations of Dyn and EM in spinal cord,and no significant difference was found between the SM group and the MC group.CONCLUSION:Moxibustion therapy can significantly enhance the pain threshold of rats with chronic visceral hyperalgesia,and the effect may be closely related to the increased concentration of Dyn and EM in spinal cord.
基金Supported by 973 Program:the Research of Law and Principle of Moxibustion(No.2009CB522905)
文摘OBJECTIVE:To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin(POMC)and prodynorphin(PDYN)mRNA expressions and plasmaβ-endorphin(β-EP)content in rheumatoid arthritis(RA)rats,to understand the mechanism of moxibustion analgesia and its dose-effect relationship.METHODS:Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal control group.The RA model was created by raising rats in a windy(blowing with electric fan),cold(6℃±2℃),and wet(80%-90%humidity)environment for 20 days,12 h each day.This was followed byinjectionofFreund'scompleteadjuvant(0.15 mL)into the ankle.Then,rats were randomly divided into a model group,moxibustion groupⅠ,and moxibustion groupⅡ,with 12 rats in each group.In moxibustion groupsⅠandⅡ,moxibustion was given at Shenshu(BL 23)and Zusanli(ST 36)for 20and 40 min,respectively,once daily for 15 days.Hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content were determined.RESULTS:Compared with the normal group,the pressure pain threshold decreased,while the hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content increased in the moxibustion groups(P<0.01).Compared with the model group,the pressure pain threshold,hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content in the moxibustion groups increased significantly(P<0.01).Compared the moxibustion groupⅠ,the pain threshold,hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content in moxibustion groupⅡsignificantly increased(P<0.01).CONCLUSION:Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasmaβ-EP content inRArats.TheanalgesiceffectinmoxibustiongroupⅡisbetterthanthatinmoxibustiongroupⅠ.
文摘It has been proved that endogenous opioids play an important pathophysiological role inthe lesions secondary to central nervous system trauma.Opiate antagonists and opioid antisera havebeen found to improve the outcome of experimental brain injury,Thyrotropin-releasing hormone(TRH),which appears to act in part as a functional antagonist of opioid system,has also beenproved to be effective in the treatment of experimental brain injury.The developments of these as-pects in our laboratory are reviewed.
基金supported by the National Basic Research Program of China(Nos.2007CB512501,2009CB522003).
文摘Drug addiction is a chronic brain disorder characterized by withdrawal symptoms that occur during drug abstinence and a high tendency of relapse.Compared with the currently available pharmacological interventions,acupuncture therapy has the potential to help drug addicts stay away from drugs without major adverse side effects.It has taken decades of research to optimize the parameters of electrical acupoint stimulation for detoxification and for relapse prevention,as well as to establish a safe and easy procedure by which drug addicts can use it on themselves.The discovery that acupuncture can trigger the release of opioid substances from the brain in the 1970s provided the inspiration.Following this,basic research on animals made it possible to understand the mechanisms of action and establish the procedure for treating drug addictions.This article reviews the past,present,and foreseeable future regarding the use of acupuncture-related technique for the treatment of opiate addiction from the perspective of translational medicine.
文摘目的研究强啡肽及P物质在尿毒症皮肤瘙痒中的相互作用。方法5／6肾大部切除模型（STNx），24周进入终末期肾功能衰竭（ESRD）。分为A：STNx＋P，B：STNx＋强，C：sTNx模型对照及D：假手术组。分别皮内注射P物质（SP）、强啡肽及生理盐水。注射后30min内观察搔抓反应。ELISA法检测血中sP浓度，免疫组化法检测皮肤组织SP表达。结果5／6肾切除大鼠血中SP升高，皮内注射SP的大鼠进一步升高，而皮内注射强啡肽后可明显降低血中SP浓度（A-D：2530．0±236．3 vs 612．4±72．2 vs 1010．2±103．5 vs 240．2±36．5）。免疫组化皮肤组织SP的表达可见到相似的趋势。STNx＋P组大鼠搔抓次数最多，B组与C组差异无统计学意义（P〉0．05）。结论尿毒症大鼠皮肤瘙痒与其血中及皮肤组织中SP表达增高有关。皮内注射强啡肽不能诱发尿毒症大鼠皮肤瘙痒反应，并能降低其血中及皮肤组织中SP的水平。
