Lorenz-RR scatter plot has an obvious shortcoming in that it does not indicate the time when the scatter point happens.On the Lorenz RR scatter plot,one cannot know the time during which the cardiac rhythms take place...Lorenz-RR scatter plot has an obvious shortcoming in that it does not indicate the time when the scatter point happens.On the Lorenz RR scatter plot,one cannot know the time during which the cardiac rhythms take place.Since occurrence of cardiac rhythms is time-related,time should be introduced to such plots.In this study,time was used as abscissa and RR interval(the time interval between the previous RR wave and the R wave)as the ordinate and time was compressed into a visually observable length,and thereby a timed RR-interval scatter plot,or t-RR scatter plot,for short,was developed.On t-RR scatter plot,the patterns were band-shaped or were of linear type.On the t-RR plot,the sinus rhythm presented bands of various widths,with the spiculate or burred upper and lower boundaries,having diurnal variation.Premature beats showed separate layers(“stratification”),the layer number corresponding the number of RR-intervals.With simple premature beats,the layers were clearly separated.With parasystole rhythm,the upper and lower bands or layers might become thicker.With arial premature beats,the space or distance between layers varied.Ventricular premature beats presented equal space or distance between layers.With tachycardia,the lower layer became a“solid”layer.With atrial fibration,the“stratification”disappeared,presenting thicker or widened layers or bands,with neat lower boundary.With atrial flutter,the layers went parallel,with the layers being evenly separated or some distances being exact multiples of others.The second degree atrioventricular block displayed two layers,the lower and upper bands being equally away from the X-axis,presenting a straight line(pacing at a fixed rate)or a thicker or wider bands,with a neat upper boundary(the lowest pacing rate).When the scatter plot presented uncharacteristic patterns or had some scattered points,which rendered diagnosis difficult,a reverse technology could be used.Briefly,upon selection of scattered points,they were subjected to computerization,by regressio展开更多
We investigated the interaction between DNA and hexammine cobalt III [Co（NH3）6]3＋ by a simple molecular combing method and dynamic light scattering. The average extension of A- DNA-YOYO-1 complex is found to be 20....We investigated the interaction between DNA and hexammine cobalt III [Co（NH3）6]3＋ by a simple molecular combing method and dynamic light scattering. The average extension of A- DNA-YOYO-1 complex is found to be 20.9μm, about 30% longer than the contour length of the DNA in TE buffer （10 mmol/L Tris, 1 mmol/L EDTA, pH=8.0）, due to bis-intercalation of YOYO-1. A multivalent cation, hexammine cobalt, is used for DNA condensation. We find that the length of DNA-[Co（NH3）6]3＋ complexes decrease from 20.9 μm to 5.9μm as the concentration of the [Co（NH3）6]3＋ vary from 0 to 3 μmol/L. This observation provides a direct visualization of single DNA condensation induced by hexammine cobalt. The results from the molecular combing studies are supported by dynamic light scattering investigation, where the average hydrodynamic radius of the DNA complex decreases from 203.8 nm to 39.26 nm under the same conditions. It shows that the molecular combing method is feasible for quantitative conformation characterization of single bio-macromolecules.展开更多
文摘Lorenz-RR scatter plot has an obvious shortcoming in that it does not indicate the time when the scatter point happens.On the Lorenz RR scatter plot,one cannot know the time during which the cardiac rhythms take place.Since occurrence of cardiac rhythms is time-related,time should be introduced to such plots.In this study,time was used as abscissa and RR interval(the time interval between the previous RR wave and the R wave)as the ordinate and time was compressed into a visually observable length,and thereby a timed RR-interval scatter plot,or t-RR scatter plot,for short,was developed.On t-RR scatter plot,the patterns were band-shaped or were of linear type.On the t-RR plot,the sinus rhythm presented bands of various widths,with the spiculate or burred upper and lower boundaries,having diurnal variation.Premature beats showed separate layers(“stratification”),the layer number corresponding the number of RR-intervals.With simple premature beats,the layers were clearly separated.With parasystole rhythm,the upper and lower bands or layers might become thicker.With arial premature beats,the space or distance between layers varied.Ventricular premature beats presented equal space or distance between layers.With tachycardia,the lower layer became a“solid”layer.With atrial fibration,the“stratification”disappeared,presenting thicker or widened layers or bands,with neat lower boundary.With atrial flutter,the layers went parallel,with the layers being evenly separated or some distances being exact multiples of others.The second degree atrioventricular block displayed two layers,the lower and upper bands being equally away from the X-axis,presenting a straight line(pacing at a fixed rate)or a thicker or wider bands,with a neat upper boundary(the lowest pacing rate).When the scatter plot presented uncharacteristic patterns or had some scattered points,which rendered diagnosis difficult,a reverse technology could be used.Briefly,upon selection of scattered points,they were subjected to computerization,by regressio
文摘We investigated the interaction between DNA and hexammine cobalt III [Co（NH3）6]3＋ by a simple molecular combing method and dynamic light scattering. The average extension of A- DNA-YOYO-1 complex is found to be 20.9μm, about 30% longer than the contour length of the DNA in TE buffer （10 mmol/L Tris, 1 mmol/L EDTA, pH=8.0）, due to bis-intercalation of YOYO-1. A multivalent cation, hexammine cobalt, is used for DNA condensation. We find that the length of DNA-[Co（NH3）6]3＋ complexes decrease from 20.9 μm to 5.9μm as the concentration of the [Co（NH3）6]3＋ vary from 0 to 3 μmol/L. This observation provides a direct visualization of single DNA condensation induced by hexammine cobalt. The results from the molecular combing studies are supported by dynamic light scattering investigation, where the average hydrodynamic radius of the DNA complex decreases from 203.8 nm to 39.26 nm under the same conditions. It shows that the molecular combing method is feasible for quantitative conformation characterization of single bio-macromolecules.
