A dual-vector system was explored for the delivery of the coagulation factor VIII gene,using intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post-translationally.A pair of eu...A dual-vector system was explored for the delivery of the coagulation factor VIII gene,using intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post-translationally.A pair of eukaryotic expression vectors,expressing Ssp DnaB intein-fused heavy and light chain genes of B-domain deleted factor VIII (BDD-FVIII),was constructed.With transient co-transfection of the two vectors into 293 and COS-7 cells,the culture supernatants contained (137±23) and (109±22) ng mL–1 spliced BDD-FVIII antigen with an activity of (1.05±0.16) and (0.79±0.23) IU mL–1 for 293 and COS-7 cells,respectively.The spliced BDD-FVIII was also detected in supernatants from a mixture of cells transfected with inteinfused heavy and light chain genes.The spliced BDD-FVIII protein bands from cell lysates were visualized by Western blotting.The data demonstrated that intein could be used to transfer the split factor VIII gene and provided valuable information on factor VIII gene delivery by dual-adeno-associated virus in hemophilia A gene therapy.展开更多
Dual throat nozzle (DTN) is fast becoming a popular technique for thrust vectoring. The DTN is designed with two throats, an upstream minimum and a downstream minimum at the nozzle exit, with a cavity in between the u...Dual throat nozzle (DTN) is fast becoming a popular technique for thrust vectoring. The DTN is designed with two throats, an upstream minimum and a downstream minimum at the nozzle exit, with a cavity in between the upstream throat and exit. In the present study, a computational work has been carried out to analyze the performance of a dual throat nozzle at various mass flow rates of secondary flow and nozzle pressure ratios (NPR). Two-dimensional, steady, compressible Navier-Stokes equations were solved using a fully implicit finite volume scheme. The present computational results were validated with available experimental data. Based on the present results, the control effectiveness of thrust-vectoring is discussed in terms of the thrust coefficient and the coefficient of discharge.展开更多
以单相级联H桥整流器(cascaded H-bridge rectifier,CHBR)为研究对象,针对传统有限集模型预测功率控制(finite-control-set model predictive power control,FCSMPPC)算法计算量大、网侧谐波分布广泛等缺点,该文首先给出一种基于两矢量...以单相级联H桥整流器(cascaded H-bridge rectifier,CHBR)为研究对象,针对传统有限集模型预测功率控制(finite-control-set model predictive power control,FCSMPPC)算法计算量大、网侧谐波分布广泛等缺点,该文首先给出一种基于两矢量的MPPC(dual-vector-based MPPC,DVB-MPPC)算法,可以实现开关频率的固定并提高稳态性能。为进一步减小计算量,提出一种DVB简化MPPC算法,取消滚动计算过程,降低控制器运算负担。在此基础上,提出一种基于负载电流计算的调制信号补偿分量注入方法,实现电容电压平衡控制,该算法无需比例积分控制器,进一步简化控制系统结构。最后,对3种MPPC算法在单相三模块CHBR实验样机上进行实验对比研究,实验结果证明了所提算法的正确性与有效性。展开更多
In this paper,we investigate some properties of dual complex numbers,dual complex vectors,and dual complex matrices.First,based on the magnitude of the dual complex number,we study the Young inequality,the Hölder...In this paper,we investigate some properties of dual complex numbers,dual complex vectors,and dual complex matrices.First,based on the magnitude of the dual complex number,we study the Young inequality,the Hölder inequality,and the Minkowski inequality in the setting of dual complex numbers.Second,we define the p-norm of a dual complex vector,which is a nonnegative dual number,and show some related properties.Third,we study the properties of eigenvalues of unitary matrices and unitary triangulation of arbitrary dual complex matrices.In particular,we introduce the operator norm of dual complex matrices induced by the p-norm of dual complex vectors,and give expressions of three important operator norms of dual complex matrices.展开更多
基金supported by the Natural Science Foundation of Shandong Province, China (Grant No. Y2005D14)the Science and Technology Program of Yantai (Grant No. 2008152)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (Grant No. 20071108)the Discipline Construction Funds of Ludong University
文摘A dual-vector system was explored for the delivery of the coagulation factor VIII gene,using intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post-translationally.A pair of eukaryotic expression vectors,expressing Ssp DnaB intein-fused heavy and light chain genes of B-domain deleted factor VIII (BDD-FVIII),was constructed.With transient co-transfection of the two vectors into 293 and COS-7 cells,the culture supernatants contained (137±23) and (109±22) ng mL–1 spliced BDD-FVIII antigen with an activity of (1.05±0.16) and (0.79±0.23) IU mL–1 for 293 and COS-7 cells,respectively.The spliced BDD-FVIII was also detected in supernatants from a mixture of cells transfected with inteinfused heavy and light chain genes.The spliced BDD-FVIII protein bands from cell lysates were visualized by Western blotting.The data demonstrated that intein could be used to transfer the split factor VIII gene and provided valuable information on factor VIII gene delivery by dual-adeno-associated virus in hemophilia A gene therapy.
文摘Dual throat nozzle (DTN) is fast becoming a popular technique for thrust vectoring. The DTN is designed with two throats, an upstream minimum and a downstream minimum at the nozzle exit, with a cavity in between the upstream throat and exit. In the present study, a computational work has been carried out to analyze the performance of a dual throat nozzle at various mass flow rates of secondary flow and nozzle pressure ratios (NPR). Two-dimensional, steady, compressible Navier-Stokes equations were solved using a fully implicit finite volume scheme. The present computational results were validated with available experimental data. Based on the present results, the control effectiveness of thrust-vectoring is discussed in terms of the thrust coefficient and the coefficient of discharge.
文摘以单相级联H桥整流器(cascaded H-bridge rectifier,CHBR)为研究对象,针对传统有限集模型预测功率控制(finite-control-set model predictive power control,FCSMPPC)算法计算量大、网侧谐波分布广泛等缺点,该文首先给出一种基于两矢量的MPPC(dual-vector-based MPPC,DVB-MPPC)算法,可以实现开关频率的固定并提高稳态性能。为进一步减小计算量,提出一种DVB简化MPPC算法,取消滚动计算过程,降低控制器运算负担。在此基础上,提出一种基于负载电流计算的调制信号补偿分量注入方法,实现电容电压平衡控制,该算法无需比例积分控制器,进一步简化控制系统结构。最后,对3种MPPC算法在单相三模块CHBR实验样机上进行实验对比研究,实验结果证明了所提算法的正确性与有效性。
基金the National Natural Science Foundation of China(Grant No.11871051).
文摘In this paper,we investigate some properties of dual complex numbers,dual complex vectors,and dual complex matrices.First,based on the magnitude of the dual complex number,we study the Young inequality,the Hölder inequality,and the Minkowski inequality in the setting of dual complex numbers.Second,we define the p-norm of a dual complex vector,which is a nonnegative dual number,and show some related properties.Third,we study the properties of eigenvalues of unitary matrices and unitary triangulation of arbitrary dual complex matrices.In particular,we introduce the operator norm of dual complex matrices induced by the p-norm of dual complex vectors,and give expressions of three important operator norms of dual complex matrices.
