Nomograms for predicting the risk of prostate cancer developed using other populations may introduce sizable bias when applied to a Chinese cohort. In the present study, we sought to develop a nomogram for predicting ...Nomograms for predicting the risk of prostate cancer developed using other populations may introduce sizable bias when applied to a Chinese cohort. In the present study, we sought to develop a nomogram for predicting the probability of a positive initial prostate biopsy in a Chinese population. A total of 535 Chinese men who underwent a prostatic biopsy for the detection of prostate cancer in the past decade with complete biopsy data were included. Stepwise logistic regression was used to determine the independent predictors of a positive initial biopsy. Age, prostate-specific antigen （PSA）, prostate volume （PV）, digital rectal examination （DRE） status, % free PSA and transrectal ultrasound （TRUS） findings were included in the analysis. A nomogram model was developed that was based on these independent predictors to calculate the probability of a positive initial prostate biopsy. A receiver-operating characteristic curve was used to assess the accuracy of using the nomogram and PSA levels alone for predicting positive prostate biopsy. The rate for positive initial prostate biopsy was 41.7% （223/535）. The independent variables used to predict a positive initial prostate biopsy were age, PSA, PV and DRE status. The areas under the receiver-operating characteristic curve for a positive initial prostate biopsy for PSA alone and the nomogram were 79.7% and 84.8%, respectively. Our results indicate that the risk of a positive initial prostate biopsy can be predicted to a satisfactory level in a Chinese population using our nomogram. The nomogram can be used to identify and counsel patients who should consider a prostate biopsy, ultimately enhancing accuracy in diagnosing prostate cancer.展开更多
Dehydration-responsive element-binding (DREB) proteins specifically binding with dehydration-responsive element (DRE) have been identified as a kind of important transcription activator of plants under drought, high s...Dehydration-responsive element-binding (DREB) proteins specifically binding with dehydration-responsive element (DRE) have been identified as a kind of important transcription activator of plants under drought, high salt and cold stress. The conserved amino, acid residues of Val (14th residue) and Glu (19th residue) in AP2/EREBP domain of DREB1A have been identified to be two key points in determining the binding ability of DREB gene with DRE element. Using the yeast one-hybrid system, we isolated one maize DREB gene named maDREB1 by screening cDNA library. Trans-activation experiment in yeast reporter strain demonstrated that maDREB1 protein could function as a DREB transcription factor activating target gene expression by specifically binding to the DRE cis-element. To assess the functional significance of these two residues in maDREB1, the V14 and E19 were substituted individually or doubly by Ala and Asp. Point mutation analysis showed that V14 substitution made significant loss of binding ability with DRE element, while point mutation of E19 had less effect. If the substitution happened simultaneously to these two residues, it would lead to great loss of the ability of binding with DRE element. It suggested that V14 and E19 were both important in protein-DNA interacting in maDREB1, though 14V was more essential. The copy number and expression pattern of maDREB1 was discussed.展开更多
The study aims to detect the subcellular localization of ERF （ethylene-responsive element binding factor） transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and...The study aims to detect the subcellular localization of ERF （ethylene-responsive element binding factor） transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS 1000/He. Transformed cells were incubated for 24 h at 22℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST （glutathione-S-transferase） in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 （DE3）. IPTG （0.5 mmol L-1） was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32p-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses （e.g., salt-） induced signaling pathway.展开更多
An improved precise integration method （IPIM） for solving the differential Riccati equation （DRE） is presented. The solution to the DRE is connected with the exponential of a Hamiltonian matrix, and the precise in...An improved precise integration method （IPIM） for solving the differential Riccati equation （DRE） is presented. The solution to the DRE is connected with the exponential of a Hamiltonian matrix, and the precise integration method （PIM） for solving the DRE is connected with the scaling and squaring method for computing the exponential of a matrix. The error analysis of the scaling and squaring method for the exponential of a matrix is applied to the PIM of the DRE. Based ,on the error analysis, the criterion for choosing two parameters of the PIM is given. Three kinds of IPIMs for solving the DRE are proposed. The numerical examples machine accuracy solutions. show that the IPIM is stable and gives the展开更多
TINY isolated through a transposon mutagenesis experiment designed to recover dominant gain of function alleles in Arabidopsis encodes a protein containing a putative DNA binding domain which is conserved in AP2...TINY isolated through a transposon mutagenesis experiment designed to recover dominant gain of function alleles in Arabidopsis encodes a protein containing a putative DNA binding domain which is conserved in AP2/EREBP transcription factors of plants. AP2/EREBP transcription factors play a variety of regulatory roles in several developmental processes and in response to biotic and environmental stresses. Using the yeast one hybrid analysis system, we demonstrated that TINY could function as a DREB like transcription factor specifically binding to the dehydration responsive element (DRE) cis acting element. 展开更多
A novel routing architecture named DREAMSCAPE is presented to solve the problem of path computation in multi-layer, multi-domain and multi-constraints scenarios, which includes Group Engine (GE) and Unit Engine (UE). ...A novel routing architecture named DREAMSCAPE is presented to solve the problem of path computation in multi-layer, multi-domain and multi-constraints scenarios, which includes Group Engine (GE) and Unit Engine (UE). GE, UE and their cooperation relationship form the main feature of DREAMSCAPE, i.e. Dual Routing Engine (DRE). Based on DRE, two routing schemes are proposed, which are DRE Forward Path Computation (DRE-FPC) and Hierarchical DRE Backward Recursive PCE-based Computation (HDRE-BRPC). In order to validate various intelligent networking technologies of large-scale heterogeneous optical networks, a DRE-based transport optical networks testbed is built with 1000 GMPLS-based control nodes and 5 optical transport nodes. The two proposed routing schemes, i.e. DRE-FPC and HDRE-BRPC, are validated on the testbed, compared with traditional Hierarchical Routing (HR) scheme. Experimental results show a good performance of DREAMSCAPE.展开更多
文摘Nomograms for predicting the risk of prostate cancer developed using other populations may introduce sizable bias when applied to a Chinese cohort. In the present study, we sought to develop a nomogram for predicting the probability of a positive initial prostate biopsy in a Chinese population. A total of 535 Chinese men who underwent a prostatic biopsy for the detection of prostate cancer in the past decade with complete biopsy data were included. Stepwise logistic regression was used to determine the independent predictors of a positive initial biopsy. Age, prostate-specific antigen （PSA）, prostate volume （PV）, digital rectal examination （DRE） status, % free PSA and transrectal ultrasound （TRUS） findings were included in the analysis. A nomogram model was developed that was based on these independent predictors to calculate the probability of a positive initial prostate biopsy. A receiver-operating characteristic curve was used to assess the accuracy of using the nomogram and PSA levels alone for predicting positive prostate biopsy. The rate for positive initial prostate biopsy was 41.7% （223/535）. The independent variables used to predict a positive initial prostate biopsy were age, PSA, PV and DRE status. The areas under the receiver-operating characteristic curve for a positive initial prostate biopsy for PSA alone and the nomogram were 79.7% and 84.8%, respectively. Our results indicate that the risk of a positive initial prostate biopsy can be predicted to a satisfactory level in a Chinese population using our nomogram. The nomogram can be used to identify and counsel patients who should consider a prostate biopsy, ultimately enhancing accuracy in diagnosing prostate cancer.
文摘Dehydration-responsive element-binding (DREB) proteins specifically binding with dehydration-responsive element (DRE) have been identified as a kind of important transcription activator of plants under drought, high salt and cold stress. The conserved amino, acid residues of Val (14th residue) and Glu (19th residue) in AP2/EREBP domain of DREB1A have been identified to be two key points in determining the binding ability of DREB gene with DRE element. Using the yeast one-hybrid system, we isolated one maize DREB gene named maDREB1 by screening cDNA library. Trans-activation experiment in yeast reporter strain demonstrated that maDREB1 protein could function as a DREB transcription factor activating target gene expression by specifically binding to the DRE cis-element. To assess the functional significance of these two residues in maDREB1, the V14 and E19 were substituted individually or doubly by Ala and Asp. Point mutation analysis showed that V14 substitution made significant loss of binding ability with DRE element, while point mutation of E19 had less effect. If the substitution happened simultaneously to these two residues, it would lead to great loss of the ability of binding with DRE element. It suggested that V14 and E19 were both important in protein-DNA interacting in maDREB1, though 14V was more essential. The copy number and expression pattern of maDREB1 was discussed.
基金financially supported by the National 863 Program of China(2007AA10Z130)the National Natural Science Foundation of China(30700504).
文摘The study aims to detect the subcellular localization of ERF （ethylene-responsive element binding factor） transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS 1000/He. Transformed cells were incubated for 24 h at 22℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST （glutathione-S-transferase） in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 （DE3）. IPTG （0.5 mmol L-1） was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32p-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses （e.g., salt-） induced signaling pathway.
基金Project supported by the National Natural Science Foundation of China(Nos.10902020 and 10721062)
文摘An improved precise integration method （IPIM） for solving the differential Riccati equation （DRE） is presented. The solution to the DRE is connected with the exponential of a Hamiltonian matrix, and the precise integration method （PIM） for solving the DRE is connected with the scaling and squaring method for computing the exponential of a matrix. The error analysis of the scaling and squaring method for the exponential of a matrix is applied to the PIM of the DRE. Based ,on the error analysis, the criterion for choosing two parameters of the PIM is given. Three kinds of IPIMs for solving the DRE are proposed. The numerical examples machine accuracy solutions. show that the IPIM is stable and gives the
基金Supported by the National Key Basic Research Science Foundation of China ( G19990 1170 3 ) the National Natural Science Foundation of China( No.3 9770 167) and the Natural Science Foundation of Tsinghua U niversity
文摘TINY isolated through a transposon mutagenesis experiment designed to recover dominant gain of function alleles in Arabidopsis encodes a protein containing a putative DNA binding domain which is conserved in AP2/EREBP transcription factors of plants. AP2/EREBP transcription factors play a variety of regulatory roles in several developmental processes and in response to biotic and environmental stresses. Using the yeast one hybrid analysis system, we demonstrated that TINY could function as a DREB like transcription factor specifically binding to the dehydration responsive element (DRE) cis acting element.
基金supported in part by National Key Basic Research Program of China (973 program) under Grant No.2010CB328204National High Technology Research and Development Program of China (863 program) under Grant No.2009AA01Z255+3 种基金National Natural Science Foundation of China under Grant No. 60932004RFDP Project under Grant No.20090005110013111 Project of China under Grant No.B07005China Fundamental Research Funds for the Central Universities
文摘A novel routing architecture named DREAMSCAPE is presented to solve the problem of path computation in multi-layer, multi-domain and multi-constraints scenarios, which includes Group Engine (GE) and Unit Engine (UE). GE, UE and their cooperation relationship form the main feature of DREAMSCAPE, i.e. Dual Routing Engine (DRE). Based on DRE, two routing schemes are proposed, which are DRE Forward Path Computation (DRE-FPC) and Hierarchical DRE Backward Recursive PCE-based Computation (HDRE-BRPC). In order to validate various intelligent networking technologies of large-scale heterogeneous optical networks, a DRE-based transport optical networks testbed is built with 1000 GMPLS-based control nodes and 5 optical transport nodes. The two proposed routing schemes, i.e. DRE-FPC and HDRE-BRPC, are validated on the testbed, compared with traditional Hierarchical Routing (HR) scheme. Experimental results show a good performance of DREAMSCAPE.
