Objective: The aim was to detect the expression of PR and CD146 in paraf-fin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of Q...Objective: The aim was to detect the expression of PR and CD146 in paraf-fin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of QDs double-labeling immunofluorescence in endometrioid adenocarcinoma. Methods: To detect the expression of PR and CD146 on 140 cases of paraffin-embedded tissue sections of endometrioid adenocarcinoma by using QDS double-labeling immunofluorescence. Results: The co-expression of PR and CD146 in the endometrioid adenocarcinoma can be detected by QDs double-labeling immunofluorescence, and there was no correlation between them (P > 0.05). Conclusion: QDs double-labeling immunofluorescence can detect the localization and co-expression of PR and CD146 in the endometrioid adenocarcinoma.展开更多
This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free mediu...This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts. Twelve weeks after transplantation, 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer DiI into the striatum results in fluorescent labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.展开更多
Aromatic carboxylic acids(ACAs) may be as transformed key metabolites via gut microbiome for playing better pharmacological effects. However, it's rare to achieve high-specificity, high-sensitivity, and highthroug...Aromatic carboxylic acids(ACAs) may be as transformed key metabolites via gut microbiome for playing better pharmacological effects. However, it's rare to achieve high-specificity, high-sensitivity, and highthroughput detection simultaneously, especially, for tracing trace ACAs in gut microbiome. In this work,firstly, a novel dual-template and double-shelled molecularly imprinted 96-well microplates(DDMIPs)was designed and amplified signal for p-hydroxybenzoic acid(PBA) and 3,4,5-trimethoxycinnamic acid(TMA). Additionally, the DDMIPs and a stable isotope labeling derivatization(SILD) method combined with the ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry(UHPLC-TQ MS) was firstly stepwise integrated, achieving high-effective, high-sensitive, and highthroughput study of gut microbiome metabolism. The whole strategy showed lower limits of detections(LODs) up to 1000 folds than the traditional method, and revealed a more real metabolism-time profile of PBA and TMA by 3-step signal amplification. The platform also laid the foundation for fast, simple,high-selective, high-effective, and high-throughput metabolism and pharmacological research.展开更多
文摘Objective: The aim was to detect the expression of PR and CD146 in paraf-fin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of QDs double-labeling immunofluorescence in endometrioid adenocarcinoma. Methods: To detect the expression of PR and CD146 on 140 cases of paraffin-embedded tissue sections of endometrioid adenocarcinoma by using QDS double-labeling immunofluorescence. Results: The co-expression of PR and CD146 in the endometrioid adenocarcinoma can be detected by QDs double-labeling immunofluorescence, and there was no correlation between them (P > 0.05). Conclusion: QDs double-labeling immunofluorescence can detect the localization and co-expression of PR and CD146 in the endometrioid adenocarcinoma.
基金Supported by the National Natural Science Foundationof China (No. 30 0 70 2 4 5 )
文摘This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts. Twelve weeks after transplantation, 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer DiI into the striatum results in fluorescent labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.
基金the National Natural Science Foundation of China (Nos. 82073973, 81872969)the Jilin Provincial Industrial Innovation Special Fund Project (No. 20200703015ZP)the Science and the Youth Innovation Promotion Association of CAS (No. 2019227)。
文摘Aromatic carboxylic acids(ACAs) may be as transformed key metabolites via gut microbiome for playing better pharmacological effects. However, it's rare to achieve high-specificity, high-sensitivity, and highthroughput detection simultaneously, especially, for tracing trace ACAs in gut microbiome. In this work,firstly, a novel dual-template and double-shelled molecularly imprinted 96-well microplates(DDMIPs)was designed and amplified signal for p-hydroxybenzoic acid(PBA) and 3,4,5-trimethoxycinnamic acid(TMA). Additionally, the DDMIPs and a stable isotope labeling derivatization(SILD) method combined with the ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry(UHPLC-TQ MS) was firstly stepwise integrated, achieving high-effective, high-sensitive, and highthroughput study of gut microbiome metabolism. The whole strategy showed lower limits of detections(LODs) up to 1000 folds than the traditional method, and revealed a more real metabolism-time profile of PBA and TMA by 3-step signal amplification. The platform also laid the foundation for fast, simple,high-selective, high-effective, and high-throughput metabolism and pharmacological research.
