Let R_Ⅲ(n) be the classical domain of type Ⅲ with n≥2. This article is devoted to a deep study of the Schwarz lemma on R_Ⅲ(n) via not only exploring the smooth boundary points of R_Ⅲ(n) but also proving the Schwa...Let R_Ⅲ(n) be the classical domain of type Ⅲ with n≥2. This article is devoted to a deep study of the Schwarz lemma on R_Ⅲ(n) via not only exploring the smooth boundary points of R_Ⅲ(n) but also proving the Schwarz lemma at the smooth boundary point for holomorphic self-mappings of R_Ⅲ(n).展开更多
AIM： To investigate the effects of mutations in domain Ⅲ of the hepatitis C virus （HCV） internal ribosome entry sequences （IRES） on the response of chronic HCV genotype 4a patients to interferon therapy.METHODS...AIM： To investigate the effects of mutations in domain Ⅲ of the hepatitis C virus （HCV） internal ribosome entry sequences （IRES） on the response of chronic HCV genotype 4a patients to interferon therapy.METHODS： HCV RNA was extracted from 19 chronic HCV 4a patients receiving interferon/ribavirin therapy who showed dramatic differences in their response to combination therapy after initial viral clearance. IRES domain Ⅲ was cloned and 15 clones for each patient were sequenced. The obtained sequences were aligned with genotype 4a prototype using the ClustaIW program and mutations scored. Prediction of stem-loop secondary structure and thermodynamic stability of the major quasispecies in each patient was performed using the MFOLD 3.2 program with Turner energies and selected constraints on base pairing.RESULTS： Analysis of RNA secondary structure revealed that insertions in domain Ⅲ altered WatsonCrick base pairing of stems and reduced molecular stability of RNA, which may ultimately reduce binding affinity to ribosomal proteins. Insertion mutations in domain - were statistically more prevalent in sustained viral response patients （SVR, n = 14） as compared to breakthrough （BT, n = 5） patients.CONCLUSION： The influence of mutations within domain Ⅲ on the response of HCV patients to combination therapy depends primarily on the position, but not the frequency, of these mutations within IRES domain Ⅲ.展开更多
Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an ...Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an environment that provides animals with multi-sensory stimulation and movement opportunities. An enriched environment has been shown to promote the regeneration of nerve cells, synapses, and blood vessels in the animal brain after cerebral ischemia;however, the exact mechanisms have not been clarified. This study aimed to determine whether an enriched environment could improve neurobehavioral functions after the experimental inducement of cerebral ischemia and whether neurobehavioral outcomes were associated with the expression of FDNC5 and BDNF. This study established ischemic mouse models using permanent middle cerebral artery occlusion(pMCAO) on the left side. On postoperative day 1, the mice were randomly assigned to either enriched environment or standard housing condition groups. Mice in the standard housing condition group were housed and fed under standard conditions. Mice in the enriched environment group were housed in a large cage, containing various toys, and fed with a standard diet. Sham-operated mice received the same procedure, but without artery occlusion, and were housed and fed under standard conditions. On postoperative days 7 and 14, a beam-walking test was used to assess coordination, balance, and spatial learning. On postoperative days 16–20, a Morris water maze test was used to assess spatial learning and memory. On postoperative day 15, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex were analyzed by western blot assay. The results showed that compared with the standard housing condition group, the motor balance and coordination functions(based on beam-walking test scores 7 and 14 days after operation), spatial learning abilities(based on the spatial learning scores from the Morris water maze test 16–19 days after operation), and memory 展开更多
农业旅游的飞速发展,对其相关研究日益引起重视,而准确把握其研究学术动态、研究热点与发展趋势等,对于推动该领域的深入研究具有重要意义。以Web of Science为数据源,采用科学图谱工具CiteSpace对农业旅游研究进行图谱分析。结果表明:1...农业旅游的飞速发展,对其相关研究日益引起重视,而准确把握其研究学术动态、研究热点与发展趋势等,对于推动该领域的深入研究具有重要意义。以Web of Science为数据源,采用科学图谱工具CiteSpace对农业旅游研究进行图谱分析。结果表明:1995年以来农业旅游研究进入蓬勃发展期,研究的规范性不断增强;研究热点丰富,7篇文献构成了研究的知识基础,研究视角从"物化"到"人化",基础到应用研究的趋势明显;显著的学科交叉性,主要有环境科学、生态学、农学;文献发表涉及刊物众多,农业类和旅游类刊物占主体;研究力量主要分布在北美、欧洲、东亚,已形成多个作者研究团队和研究机构,中国的研究力量居世界前列,但宜加强国际合作与交流。展开更多
文摘根据GenBank中发表的西尼罗病毒(WNV)E domainⅢ基因序列设计1对引物,用PCR方法扩增domainⅢ基因片段,回收目的基因片段定向克隆入果蝇表达载体pMT/B/V5,构建重组表达载体pMT-DⅢ。测序验证正确后,用脂质体转染法与辅助质粒pCoBlast共转染果蝇S2细胞,通过Blasticidin进行加压筛选,获得了抗性细胞S2-DⅢ。提取S2-DⅢ细胞的基因组DNA,用PCR方法检测目的基因在果蝇S2细胞中的整合与表达。用硫酸铜溶液诱导表达,收集无血清的细胞表达上清,样品浓缩后进行SDS-PAGE及Western blotting鉴定。结果表明,研究成功构建了重组表达载体pMT-DⅢ,转染细胞经12mg/L的Blasticidin筛选及鉴定后获得了稳定表达WNV E do-mainⅢ蛋白的果蝇S2细胞株。
基金supported by the National Natural Science Foundation of China(Nos.11571105,11771139)。
文摘Let R_Ⅲ(n) be the classical domain of type Ⅲ with n≥2. This article is devoted to a deep study of the Schwarz lemma on R_Ⅲ(n) via not only exploring the smooth boundary points of R_Ⅲ(n) but also proving the Schwarz lemma at the smooth boundary point for holomorphic self-mappings of R_Ⅲ(n).
基金Supported by A grant from National Research Center to M.Awady and grant from Yousef Jameel Science&Technology Research Center(YJ-STRC)at the American University in Cairo to H.Azzazy
文摘AIM： To investigate the effects of mutations in domain Ⅲ of the hepatitis C virus （HCV） internal ribosome entry sequences （IRES） on the response of chronic HCV genotype 4a patients to interferon therapy.METHODS： HCV RNA was extracted from 19 chronic HCV 4a patients receiving interferon/ribavirin therapy who showed dramatic differences in their response to combination therapy after initial viral clearance. IRES domain Ⅲ was cloned and 15 clones for each patient were sequenced. The obtained sequences were aligned with genotype 4a prototype using the ClustaIW program and mutations scored. Prediction of stem-loop secondary structure and thermodynamic stability of the major quasispecies in each patient was performed using the MFOLD 3.2 program with Turner energies and selected constraints on base pairing.RESULTS： Analysis of RNA secondary structure revealed that insertions in domain Ⅲ altered WatsonCrick base pairing of stems and reduced molecular stability of RNA, which may ultimately reduce binding affinity to ribosomal proteins. Insertion mutations in domain - were statistically more prevalent in sustained viral response patients （SVR, n = 14） as compared to breakthrough （BT, n = 5） patients.CONCLUSION： The influence of mutations within domain Ⅲ on the response of HCV patients to combination therapy depends primarily on the position, but not the frequency, of these mutations within IRES domain Ⅲ.
基金国家高技术研究发展计划(863计划)，国家科技重大专项，福建省医学创新课题，National High-tech R&D Program of China (863 Program)，National Science and Technology Major Project of China，Fujian Provincial Medical Innovation Project
基金supported by the National Natural Science Foundation of China,Nos.81601961(to KWY),81672242(to YW)the Key Construction Projects of Shanghai Health and Family Planning on Weak Discipline,China,No.2015ZB0401(to YW)
文摘Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an environment that provides animals with multi-sensory stimulation and movement opportunities. An enriched environment has been shown to promote the regeneration of nerve cells, synapses, and blood vessels in the animal brain after cerebral ischemia;however, the exact mechanisms have not been clarified. This study aimed to determine whether an enriched environment could improve neurobehavioral functions after the experimental inducement of cerebral ischemia and whether neurobehavioral outcomes were associated with the expression of FDNC5 and BDNF. This study established ischemic mouse models using permanent middle cerebral artery occlusion(pMCAO) on the left side. On postoperative day 1, the mice were randomly assigned to either enriched environment or standard housing condition groups. Mice in the standard housing condition group were housed and fed under standard conditions. Mice in the enriched environment group were housed in a large cage, containing various toys, and fed with a standard diet. Sham-operated mice received the same procedure, but without artery occlusion, and were housed and fed under standard conditions. On postoperative days 7 and 14, a beam-walking test was used to assess coordination, balance, and spatial learning. On postoperative days 16–20, a Morris water maze test was used to assess spatial learning and memory. On postoperative day 15, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex were analyzed by western blot assay. The results showed that compared with the standard housing condition group, the motor balance and coordination functions(based on beam-walking test scores 7 and 14 days after operation), spatial learning abilities(based on the spatial learning scores from the Morris water maze test 16–19 days after operation), and memory
文摘农业旅游的飞速发展,对其相关研究日益引起重视,而准确把握其研究学术动态、研究热点与发展趋势等,对于推动该领域的深入研究具有重要意义。以Web of Science为数据源,采用科学图谱工具CiteSpace对农业旅游研究进行图谱分析。结果表明:1995年以来农业旅游研究进入蓬勃发展期,研究的规范性不断增强;研究热点丰富,7篇文献构成了研究的知识基础,研究视角从"物化"到"人化",基础到应用研究的趋势明显;显著的学科交叉性,主要有环境科学、生态学、农学;文献发表涉及刊物众多,农业类和旅游类刊物占主体;研究力量主要分布在北美、欧洲、东亚,已形成多个作者研究团队和研究机构,中国的研究力量居世界前列,但宜加强国际合作与交流。
