建立多西紫杉醇(docetaxel,DOC)人血清白蛋白纳米粒(human serum album in nanoparticles,HSA-NP)中药物含量测定的方法。利用半胱氨酸(cysteine,Cys)还原人血清白蛋白中交联的二硫键,释放HSA-NP中包裹的药物,再利用HPLC测定药物含量。...建立多西紫杉醇(docetaxel,DOC)人血清白蛋白纳米粒(human serum album in nanoparticles,HSA-NP)中药物含量测定的方法。利用半胱氨酸(cysteine,Cys)还原人血清白蛋白中交联的二硫键,释放HSA-NP中包裹的药物,再利用HPLC测定药物含量。结果表明,在本实验条件下Cys及制剂中辅料对DOC的含量测定无干扰。1μg/LCys、37℃孵化30min,最后用乙腈沉淀蛋白的方法可以完全测定出制剂中DOC含量。展开更多
A novel thiol-derivative porphyrin[2,7,12,18-tetramethyl-13,17-di（3-disulfidepropyl）porphyrin]bearing the symmetrical disulphide bond and its metal complexes have been successfully prepared by means of modification ...A novel thiol-derivative porphyrin[2,7,12,18-tetramethyl-13,17-di（3-disulfidepropyl）porphyrin]bearing the symmetrical disulphide bond and its metal complexes have been successfully prepared by means of modification on naturally easily derived heme.The results are described by MS-MS and UV-vis spectroscopy.展开更多
文摘建立多西紫杉醇(docetaxel,DOC)人血清白蛋白纳米粒(human serum album in nanoparticles,HSA-NP)中药物含量测定的方法。利用半胱氨酸(cysteine,Cys)还原人血清白蛋白中交联的二硫键,释放HSA-NP中包裹的药物,再利用HPLC测定药物含量。结果表明,在本实验条件下Cys及制剂中辅料对DOC的含量测定无干扰。1μg/LCys、37℃孵化30min,最后用乙腈沉淀蛋白的方法可以完全测定出制剂中DOC含量。
基金support from Jiangsu Natural Science Foundation(No.BK2009386)Science and Technology Development Foundation of Nanjing University of Science and Technology(No.XKF 09008 and No.2010GJPY043)
文摘A novel thiol-derivative porphyrin[2,7,12,18-tetramethyl-13,17-di（3-disulfidepropyl）porphyrin]bearing the symmetrical disulphide bond and its metal complexes have been successfully prepared by means of modification on naturally easily derived heme.The results are described by MS-MS and UV-vis spectroscopy.
