Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided i...Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided into 4 groups of 8： normal, model, YHD, and maprotiline. The model group, YHD group and maprotiline group used separate feeding and rats were exposed to chronic and unpredictable stress to build the depression model. From day 2, the YHD group and maprotiline group were respectively given YHD （7 g/kg） and maprotiline （10 mg/kg） by gastrogavage once daily. The normal and model groups were given the same volume of drinking water. The medication duration were 21 days. At the end of the experiment, the serum levels of copper and zinc were determined by atomic absorption spectroscopy, plasma concentrations of adrenocorticotropic hormone （ACTH） and cortisol （COR） were detected by radioimmunoassay, and levels of norepinephrine （NE）, dopamine （DA）, and 5-hydroxytryptamine （5-HT） in the hypothalamus were analysed by high performance liquid chromatography-eletricochemistry. Results： Compared with the content of copper and zinc in the serum of rats in the normal group, serum copper levels in model rats were significantly increased and zinc content was significantly reduced （both P〈0.05）. Plasma concentrations of ACTH and COR in the model group were significantly increased compared with those in the normal group （P〈0.05, P〈0.01）. The contents of NE, DA, and 5-HT in the hypothalamus of rats in the model group were significantly reduced compared with those of the normal group （P〈0.05 or P〈0.01）. Compared with those in the model group, the serum copper content and plasma concentrations of ACTH and COR were significantly decreased （all P〈0.05）; meanwhile, serum zinc content and hypothalamic contents of NE, DA, and 5-HT were significantly increased in rats of the YHD group （all P〈0.05）. The same effects were also shown in the maprotiline group e展开更多
文摘目的明确脑内神经递质变化对高原睡眠障碍的影响。方法对解放军驻迪庆高原海拔3800 m和驻昆明海拔1800 m的部队分别进行匹兹堡睡眠质量指数(PSQI)、汉密尔顿焦虑(HAMA)和抑郁量表(HAMD)评定,采用脑涨落图分析仪无创性检测所有受试者脑内神经递质。采用析因分析明确睡眠质量和海拔高度的交互作用,采用多因素Logistic回归分析高原睡眠障碍的独立预测因素。结果共调查261人,最终纳入134人,均为男性。两地区被调查对象的年龄、文化程度、婚姻状况、HAMA、HAMD总分均无统计学差异(P>0.05)。高原组PSQI评分高于非高原组(7.02±2.40 vs 6.71±3.04,P=0.034.),睡眠障碍发生率为75.86%,(84/134),睡眠障碍者各项神经递质功率和兴奋抑制指数均低于睡眠正常者(P<0.05)。非高原地区,睡眠障碍者仅γ-氨基丁酸功率、谷氨酸功率和总功率低于睡眠正常者(P<0.05)。睡眠质量和海拔高度对各项神经递质实测功率均存在交互协同作用(P<0.05)。HAMD评分、谷氨酸和5-羟色胺功率增高是导致高原睡眠障碍的独立预测因素(P<0.05),γ-氨基丁酸和多巴胺功率增高则可能对高原睡眠质量有促进作用(P<0.05)。结论高原环境下,睡眠障碍者脑内各项神经递质功率均显著降低,这种变化与睡眠质量下降和海拔高度变化存在交互协同效应。脑内谷氨酸和5-羟色胺实测功率增高是导致高原睡眠障碍的独立预测因素,γ-氨基丁酸和多巴胺实测功率增高可能对高原睡眠质量有促进作用。
基金Supported by the Science Foundation of Fujian Province(No.2010J01239)the Xiamen Science and Technology Key Program Grant(No.3502Z20100006)Hong Kong Healthy Walker Co.,Ltd(No.XDTH2009023A)
文摘Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided into 4 groups of 8： normal, model, YHD, and maprotiline. The model group, YHD group and maprotiline group used separate feeding and rats were exposed to chronic and unpredictable stress to build the depression model. From day 2, the YHD group and maprotiline group were respectively given YHD （7 g/kg） and maprotiline （10 mg/kg） by gastrogavage once daily. The normal and model groups were given the same volume of drinking water. The medication duration were 21 days. At the end of the experiment, the serum levels of copper and zinc were determined by atomic absorption spectroscopy, plasma concentrations of adrenocorticotropic hormone （ACTH） and cortisol （COR） were detected by radioimmunoassay, and levels of norepinephrine （NE）, dopamine （DA）, and 5-hydroxytryptamine （5-HT） in the hypothalamus were analysed by high performance liquid chromatography-eletricochemistry. Results： Compared with the content of copper and zinc in the serum of rats in the normal group, serum copper levels in model rats were significantly increased and zinc content was significantly reduced （both P〈0.05）. Plasma concentrations of ACTH and COR in the model group were significantly increased compared with those in the normal group （P〈0.05, P〈0.01）. The contents of NE, DA, and 5-HT in the hypothalamus of rats in the model group were significantly reduced compared with those of the normal group （P〈0.05 or P〈0.01）. Compared with those in the model group, the serum copper content and plasma concentrations of ACTH and COR were significantly decreased （all P〈0.05）; meanwhile, serum zinc content and hypothalamic contents of NE, DA, and 5-HT were significantly increased in rats of the YHD group （all P〈0.05）. The same effects were also shown in the maprotiline group e
