Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Meth...Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Methods A prospectively controlled study was performed in two different medical areas in Peking Union Medical College Hospital （PUMCH） for 16 months （from May 2006 to September 2007）. In test group, a strict blood collection procedure was carried out by trained nurses with the veinpuncture sites were scrupulously disinfected with 2.5% tincture of iodine plus 70% alcohol. In control group, commonly used procedure in PUMCH was performed with 0.45% chlorhexidine acetate plus 0.2% iodine. Blood culture positive results for 4 target organisms （Coagulase-negafive staphylococci, Propionibacterium acnes, physicians from infectious department to （contamination）. Corynebacterium species and Bacillus determine whether a sample was true species） were further assessed by positive （pathogen） or false positive Results Total 9321 blood culture collections were analyzed. The blood culture contamination rate in test group was significantly lower than that in control group （5/3177 （0.16%） vs. 77/6144 （1.25%）; x2=13.382, P 〈0.001）. The most common contaminant was Coagulase-negative staphylococcus （76.83%）. The average cultural time during which contaminated samples became positive was longer than that for true pathogen samples （42.0 hours vs. 13.9 hours, P=-0.041）. Conclusion Using a strict blood collection procedure can significantly reduce blood culture contamination rate.展开更多
山桐子是有重要应用前景的能源树种,然而自然条件下休眠期长、萌发困难、发芽率极低,严重影响其推广应用。为了提高山桐子的萌发效率,笔者研究了不同消毒剂（氯化汞、过氧化氢）、消毒时间、处理方法（硫酸、超声波处理、低温层积）对...山桐子是有重要应用前景的能源树种,然而自然条件下休眠期长、萌发困难、发芽率极低,严重影响其推广应用。为了提高山桐子的萌发效率,笔者研究了不同消毒剂（氯化汞、过氧化氢）、消毒时间、处理方法（硫酸、超声波处理、低温层积）对山桐子种子消毒和促进萌发的作用。结果表明：70%乙醇结合10 min 30%过氧化氢处理,无菌苗种子污染率为0,第15天发芽率达到90%;而利用低温层积结合5-10 min 20%过氧化氢处理,实生苗种子的发芽率大幅提高,第22天发芽率达到78%。展开更多
文摘Background Blood culture contamination is a significant adverse event. The aim of this project was to evaluate the efficacy of a strict blood collection procedure in reducing the blood culture contamination rate. Methods A prospectively controlled study was performed in two different medical areas in Peking Union Medical College Hospital （PUMCH） for 16 months （from May 2006 to September 2007）. In test group, a strict blood collection procedure was carried out by trained nurses with the veinpuncture sites were scrupulously disinfected with 2.5% tincture of iodine plus 70% alcohol. In control group, commonly used procedure in PUMCH was performed with 0.45% chlorhexidine acetate plus 0.2% iodine. Blood culture positive results for 4 target organisms （Coagulase-negafive staphylococci, Propionibacterium acnes, physicians from infectious department to （contamination）. Corynebacterium species and Bacillus determine whether a sample was true species） were further assessed by positive （pathogen） or false positive Results Total 9321 blood culture collections were analyzed. The blood culture contamination rate in test group was significantly lower than that in control group （5/3177 （0.16%） vs. 77/6144 （1.25%）; x2=13.382, P 〈0.001）. The most common contaminant was Coagulase-negative staphylococcus （76.83%）. The average cultural time during which contaminated samples became positive was longer than that for true pathogen samples （42.0 hours vs. 13.9 hours, P=-0.041）. Conclusion Using a strict blood collection procedure can significantly reduce blood culture contamination rate.
文摘山桐子是有重要应用前景的能源树种,然而自然条件下休眠期长、萌发困难、发芽率极低,严重影响其推广应用。为了提高山桐子的萌发效率,笔者研究了不同消毒剂（氯化汞、过氧化氢）、消毒时间、处理方法（硫酸、超声波处理、低温层积）对山桐子种子消毒和促进萌发的作用。结果表明：70%乙醇结合10 min 30%过氧化氢处理,无菌苗种子污染率为0,第15天发芽率达到90%;而利用低温层积结合5-10 min 20%过氧化氢处理,实生苗种子的发芽率大幅提高,第22天发芽率达到78%。
