目的:观察温胃调肠颗粒对便秘型肠易激综合征(IBS-C)模型大鼠的改善作用,并探讨其可能的作用机制。方法:将60只SD大鼠随机分为正常组、模型组、莫沙必利组(阳性药物,50 mg/kg)和温胃调肠颗粒低、中、高剂量组(3、6、12 g/kg),每组10只...目的:观察温胃调肠颗粒对便秘型肠易激综合征(IBS-C)模型大鼠的改善作用,并探讨其可能的作用机制。方法:将60只SD大鼠随机分为正常组、模型组、莫沙必利组(阳性药物,50 mg/kg)和温胃调肠颗粒低、中、高剂量组(3、6、12 g/kg),每组10只。除正常组外,其余各组大鼠ig冰水混合物复制IBS-C模型。造模后,各给药组大鼠ig相应药物,正常组和模型组大鼠ig等体积生理盐水,每天1次,连续14 d。给药结束后,测定大鼠24 h排便粒数、粪便含水率;采用酶联免疫吸附法测定大鼠血清中5-羟色胺(5-HT)和降钙素基因相关肽(CGRP)的含量;分别采用实时荧光定量聚合酶链式反应法和Western blot法测定大鼠结肠组织中5-羟色胺转运蛋白(SERT)、CGRP m RNA及其蛋白表达水平。结果:与正常组比较,模型组大鼠24 h排便粒数、粪便含水率减少,血清中5-HT和CGRP含量增加,结肠组织中SERT和CGRP m RNA及其蛋白水平升高(P<0.05或P<0.01);与模型组比较,各给药组大鼠上述指标均得到不同程度改善,除温胃调肠颗粒低剂量组大鼠结肠组织SERT m RNA降低不显著外,其余各组差异均有统计学意义(P<0.05或P<0.01)。结论:温胃调肠颗粒可促进IBS-C模型大鼠的排便,改善便秘症状;其机制可能与降低血清和肠组织中5-HT、CGRP等神经递质的水平有关。展开更多
With the startup and execution of new socialistic countryside construction in Heilongjiang Province,the transition from castoff to resource is strengthened in the countryside,aiming at neat appearance of the countrysi...With the startup and execution of new socialistic countryside construction in Heilongjiang Province,the transition from castoff to resource is strengthened in the countryside,aiming at neat appearance of the countryside,clean production and saving energy.People produce biogas and provide the countryside with new energy by means of turning livestock＇s dejection into resources,composting of the plant and animal＇s leavings in the courtyard and even in the factory.It is helpful for the countryside to conserve the energy and reduce emission of the waste.And it also plays an important role in protecting the eco-environment,beautifying homestead and developing the ecological agriculture and so on.The liquid and solid residue in the biogas production can be reused as fertilizer for crops or food for animals after pretreatment,which is propitious to accelerate the development of the circular economy in Heilongjiang Province.展开更多
目的探讨沙库巴曲缬沙坦对比血管紧张素转换酶抑制剂/血管紧张素受体拮抗剂(ACEI/ARB)治疗射血分数降低的心力衰竭(HFrEF)的疗效与安全性。方法系统检索Cochrane、PubMed、Web of Science、Embase、CNKI、万方、维普等数据库中关于沙库...目的探讨沙库巴曲缬沙坦对比血管紧张素转换酶抑制剂/血管紧张素受体拮抗剂(ACEI/ARB)治疗射血分数降低的心力衰竭(HFrEF)的疗效与安全性。方法系统检索Cochrane、PubMed、Web of Science、Embase、CNKI、万方、维普等数据库中关于沙库巴曲缬沙坦与ACEI/ARB治疗HFrEF的随机对照实验,用Meta分析方法分析比较两组心血管疾病病死率、HF住院风险、全因病死率,以及不良事件(低血压、高钾血症、肾损伤、血管性水肿)的发生情况。结果共纳入7项研究,Meta分析结果显示:与ACEI/ARB相比,使用沙库巴曲缬沙坦能显著降低HFrEF患者的心血管疾病病死率(RR=0.81,95%CI:0.73~0.90,P<0.01),HF住院风险(RR=0.78,95%CI:0.71~0.86,P<0.01),全因病死率(RR=0.85,95%CI:0.78~0.93,P<0.01)。在不良事件发生方面,与ACEI/ARB相比,沙库巴曲缬沙坦组的肾损伤(RR=0.80,P=0.01)、高钾血症(RR=0.91,P=0.03)发生率明显降低,但增加了低血压(RR=1.54,P<0.01)发生风险。结论沙库巴曲缬沙坦代替ACEI/ARB治疗HFrEF能显著降低心血管终点事件,并且安全性良好。展开更多
文摘目的:观察温胃调肠颗粒对便秘型肠易激综合征(IBS-C)模型大鼠的改善作用,并探讨其可能的作用机制。方法:将60只SD大鼠随机分为正常组、模型组、莫沙必利组(阳性药物,50 mg/kg)和温胃调肠颗粒低、中、高剂量组(3、6、12 g/kg),每组10只。除正常组外,其余各组大鼠ig冰水混合物复制IBS-C模型。造模后,各给药组大鼠ig相应药物,正常组和模型组大鼠ig等体积生理盐水,每天1次,连续14 d。给药结束后,测定大鼠24 h排便粒数、粪便含水率;采用酶联免疫吸附法测定大鼠血清中5-羟色胺(5-HT)和降钙素基因相关肽(CGRP)的含量;分别采用实时荧光定量聚合酶链式反应法和Western blot法测定大鼠结肠组织中5-羟色胺转运蛋白(SERT)、CGRP m RNA及其蛋白表达水平。结果:与正常组比较,模型组大鼠24 h排便粒数、粪便含水率减少,血清中5-HT和CGRP含量增加,结肠组织中SERT和CGRP m RNA及其蛋白水平升高(P<0.05或P<0.01);与模型组比较,各给药组大鼠上述指标均得到不同程度改善,除温胃调肠颗粒低剂量组大鼠结肠组织SERT m RNA降低不显著外,其余各组差异均有统计学意义(P<0.05或P<0.01)。结论:温胃调肠颗粒可促进IBS-C模型大鼠的排便,改善便秘症状;其机制可能与降低血清和肠组织中5-HT、CGRP等神经递质的水平有关。
基金Supported by Foundation of Heilongjiang Province Educational Committee (11551056)Scientific Fund of Heilongjiang Province for Youth (QC2009C40)+1 种基金Fund of Harbin City Innovative Talent (2009RFQXN096)Heilongjiang Province Postdoctoral Science Foundation
文摘With the startup and execution of new socialistic countryside construction in Heilongjiang Province,the transition from castoff to resource is strengthened in the countryside,aiming at neat appearance of the countryside,clean production and saving energy.People produce biogas and provide the countryside with new energy by means of turning livestock＇s dejection into resources,composting of the plant and animal＇s leavings in the courtyard and even in the factory.It is helpful for the countryside to conserve the energy and reduce emission of the waste.And it also plays an important role in protecting the eco-environment,beautifying homestead and developing the ecological agriculture and so on.The liquid and solid residue in the biogas production can be reused as fertilizer for crops or food for animals after pretreatment,which is propitious to accelerate the development of the circular economy in Heilongjiang Province.
文摘目的探讨沙库巴曲缬沙坦对比血管紧张素转换酶抑制剂/血管紧张素受体拮抗剂(ACEI/ARB)治疗射血分数降低的心力衰竭(HFrEF)的疗效与安全性。方法系统检索Cochrane、PubMed、Web of Science、Embase、CNKI、万方、维普等数据库中关于沙库巴曲缬沙坦与ACEI/ARB治疗HFrEF的随机对照实验,用Meta分析方法分析比较两组心血管疾病病死率、HF住院风险、全因病死率,以及不良事件(低血压、高钾血症、肾损伤、血管性水肿)的发生情况。结果共纳入7项研究,Meta分析结果显示:与ACEI/ARB相比,使用沙库巴曲缬沙坦能显著降低HFrEF患者的心血管疾病病死率(RR=0.81,95%CI:0.73~0.90,P<0.01),HF住院风险(RR=0.78,95%CI:0.71~0.86,P<0.01),全因病死率(RR=0.85,95%CI:0.78~0.93,P<0.01)。在不良事件发生方面,与ACEI/ARB相比,沙库巴曲缬沙坦组的肾损伤(RR=0.80,P=0.01)、高钾血症(RR=0.91,P=0.03)发生率明显降低,但增加了低血压(RR=1.54,P<0.01)发生风险。结论沙库巴曲缬沙坦代替ACEI/ARB治疗HFrEF能显著降低心血管终点事件,并且安全性良好。
