Background:Angiostrongyliasis is a food-borne parasitic zoonosis.Human infection is caused by infection with the third-stage larvae of Angiostrongylus cantonensis.The life cycle of A.cantonensis involves rodents as de...Background:Angiostrongyliasis is a food-borne parasitic zoonosis.Human infection is caused by infection with the third-stage larvae of Angiostrongylus cantonensis.The life cycle of A.cantonensis involves rodents as definitive hosts and molluscs as intermediate hosts.This study aims to investigate on the infection status and characteristics of spatial distribution of these hosts,which are key components in the strategy for the prevention and control of angiostrongyliasis.Methods:Three villages from Nanao Island,Guangdong Province,China,were chosen as study area by stratified random sampling.The density and natural infection of Pomacea canaliculata and various rat species were surveyed every three months from December 2015 to September 2016,with spatial correlations of the positive P.canaliculata and the infection rates analysed by ArcGIS,scan statistics,ordinary least squares(OLS)and geographically weighted regression(GWR)models.Results:A total of 2192 P.canaliculata specimens were collected from the field,of which 1190 were randomly chosen to be examined for third-stage larvae of A.cantonensis.Seventy-two Angiostrongylus-infected snails were found,which represents a larval infection rate of 6.1%(72/1190).In total,110 rats including 85 Rattus norvegicus,10 R.flavipectus,one R.losea and 14 Suncus murinus were captured,and 32 individuals were positive(for adult worms),representing an infection rate of 29.1%of the definitive hosts(32/110).Worms were only found in R.norvegicus and R.flavipectus,representing a prevalence of 36.5%(31/85)and 10%(1/10),respectively in these species,but none in R.losea and S.murinus,despite testing as many as 32 of the latter species.Statistically,spatial correlation and spatial clusters in the spatial distribution of positive P.canaliculata and positive rats existed.Most of the spatial variability of the host infection rates came from spatial autocorrelation.Nine spatial clusters with respect to positive P.canaliculata were identified,but only two correlated to infection rates.The results展开更多
目的验证PCR方法检测终宿主家犬感染细粒棘球绦虫的特异性及其在临床诊断中的应用价值。方法取感染细粒棘球绦虫家犬粪便,显微镜下计数细粒棘球绦虫虫卵,稀释后PCR检测其敏感性。同时取家犬小肠内水泡带绦虫、多头绦虫、羊绦虫,将其DNA...目的验证PCR方法检测终宿主家犬感染细粒棘球绦虫的特异性及其在临床诊断中的应用价值。方法取感染细粒棘球绦虫家犬粪便,显微镜下计数细粒棘球绦虫虫卵,稀释后PCR检测其敏感性。同时取家犬小肠内水泡带绦虫、多头绦虫、羊绦虫,将其DNA与细粒棘球绦虫DNA一起进行PCR扩增,观察其特异性。对133 bp的目标条带进行序列测定,对比分析。结果PCR检测终宿主家犬感染细粒棘球绦虫具有高度的灵敏性,即使粪便中有1个虫卵(8pg的DNA),也能测出,但扩增后特异性较差,无法利用扩增带型进行诊断。4种寄生虫均具有相同的133 bp DNA重复序列,在400 bp均可检测出相同条带。结论PCR方法检测终宿主感染细粒棘球绦虫DNA重复序列尽管有优良的灵敏性,因其特异性差,不适合用于临床诊断和推广。展开更多
Background Felids are the only definitive hosts ofToxoplasma gondii. However, the biological features of the feline small intestine followingT. gondii infection are poorly understood. We investigated the changes in th...Background Felids are the only definitive hosts ofToxoplasma gondii. However, the biological features of the feline small intestine followingT. gondii infection are poorly understood. We investigated the changes in the expression of RNAs (including mRNAs, long non-coding RNAs and circular RNAs) in the small intestinal epithelia of cats followingT. gondii infection to improve our understanding of the life cycle ofT. gondii and cat responses toT. gondii infection.Methods Fifteen cats were randomly assigned to five groups, and the infection groups were inoculated with 600 tissue cysts of theT. gondii Pru strain by gavage. The small intestinal epithelia of cats were collected at 6, 10, 14, and 30 days post infection (DPI). Using high-throughput RNA sequencing (RNA-seq), we investigated the changes in RNA expression. The expression levels of differentially expressed (DE) genes and non-coding RNAs (ncRNAs) identified by RNA-seq were validated by quantitative reverse transcription PCR (qRT-PCR). Differential expression was determined using the DESeq R package.Results In total, 207 annotated lncRNAs, 20,552 novel lncRNAs, 3342 novel circRNAs and 19,409 mRNAs were identified. Among these, 70 to 344 DE mRNAs, lncRNAs and circRNAs were detected, and the post-cleavage binding sites between 725 ncRNAs and 2082 miRNAs were predicted. Using the co-location method, we predicted that a total of 235 lncRNAs target 1044 protein-coding genes, while the results of co-expression analysis revealed that 174 lncRNAs target 2097 mRNAs. Pathway enrichment analyses of the genes targeted by ncRNAs suggested that most ncRNAs were significantly enriched in immune or diseases-related pathways. NcRNA regulatory networks revealed that a single ncRNA could be directly or indirectly regulated by multiple genes or ncRNAs that could influence the immune response of cats. Co-expression analysis showed that 242 circRNAs, mainly involved in immune responses, were significantly associated withT. gondii infection. In contrast, 1352 protein coding RNAs, ma展开更多
基金This study was funded by The Special Foundation of Basic Science and Technology Resources Survey of Ministry of Science and Technology of China(No.2017FY101203)the National Key Research and Development Program of China(No.2016YFC1202000,2016YFC1202001)the National Science and Technology Major Project of China(No.2012ZX1004–220,2008ZX1004–011).
文摘Background:Angiostrongyliasis is a food-borne parasitic zoonosis.Human infection is caused by infection with the third-stage larvae of Angiostrongylus cantonensis.The life cycle of A.cantonensis involves rodents as definitive hosts and molluscs as intermediate hosts.This study aims to investigate on the infection status and characteristics of spatial distribution of these hosts,which are key components in the strategy for the prevention and control of angiostrongyliasis.Methods:Three villages from Nanao Island,Guangdong Province,China,were chosen as study area by stratified random sampling.The density and natural infection of Pomacea canaliculata and various rat species were surveyed every three months from December 2015 to September 2016,with spatial correlations of the positive P.canaliculata and the infection rates analysed by ArcGIS,scan statistics,ordinary least squares(OLS)and geographically weighted regression(GWR)models.Results:A total of 2192 P.canaliculata specimens were collected from the field,of which 1190 were randomly chosen to be examined for third-stage larvae of A.cantonensis.Seventy-two Angiostrongylus-infected snails were found,which represents a larval infection rate of 6.1%(72/1190).In total,110 rats including 85 Rattus norvegicus,10 R.flavipectus,one R.losea and 14 Suncus murinus were captured,and 32 individuals were positive(for adult worms),representing an infection rate of 29.1%of the definitive hosts(32/110).Worms were only found in R.norvegicus and R.flavipectus,representing a prevalence of 36.5%(31/85)and 10%(1/10),respectively in these species,but none in R.losea and S.murinus,despite testing as many as 32 of the latter species.Statistically,spatial correlation and spatial clusters in the spatial distribution of positive P.canaliculata and positive rats existed.Most of the spatial variability of the host infection rates came from spatial autocorrelation.Nine spatial clusters with respect to positive P.canaliculata were identified,but only two correlated to infection rates.The results
文摘目的验证PCR方法检测终宿主家犬感染细粒棘球绦虫的特异性及其在临床诊断中的应用价值。方法取感染细粒棘球绦虫家犬粪便,显微镜下计数细粒棘球绦虫虫卵,稀释后PCR检测其敏感性。同时取家犬小肠内水泡带绦虫、多头绦虫、羊绦虫,将其DNA与细粒棘球绦虫DNA一起进行PCR扩增,观察其特异性。对133 bp的目标条带进行序列测定,对比分析。结果PCR检测终宿主家犬感染细粒棘球绦虫具有高度的灵敏性,即使粪便中有1个虫卵(8pg的DNA),也能测出,但扩增后特异性较差,无法利用扩增带型进行诊断。4种寄生虫均具有相同的133 bp DNA重复序列,在400 bp均可检测出相同条带。结论PCR方法检测终宿主感染细粒棘球绦虫DNA重复序列尽管有优良的灵敏性,因其特异性差,不适合用于临床诊断和推广。
基金Project support was kindly provided by the National Key Research and Development Program of China(Grant Nos.2021YFC2300800 and 2021YFC2300802)the National Natural Science Foundation of China(Grant Nos.32172887 and 32102701)+4 种基金the Youth Science and Technology Fund Program of Gansu province(Grant No.23JRRA562)the Innovation Project of Chinese Academy of Agricultural Sciences(Grant No.25-LZIHPS-05)the Agricultural Science and Technology Innovation Program(ASTIP)(Grant No.CAAS-ASTIP-2016-LVRI-03)the Yunnan Expert Workstation(Grant No.202005AF150041)The funding bodies played no role in the design of the study and collection,analysis,and interpretation of data and in writing the manuscript.
文摘Background Felids are the only definitive hosts ofToxoplasma gondii. However, the biological features of the feline small intestine followingT. gondii infection are poorly understood. We investigated the changes in the expression of RNAs (including mRNAs, long non-coding RNAs and circular RNAs) in the small intestinal epithelia of cats followingT. gondii infection to improve our understanding of the life cycle ofT. gondii and cat responses toT. gondii infection.Methods Fifteen cats were randomly assigned to five groups, and the infection groups were inoculated with 600 tissue cysts of theT. gondii Pru strain by gavage. The small intestinal epithelia of cats were collected at 6, 10, 14, and 30 days post infection (DPI). Using high-throughput RNA sequencing (RNA-seq), we investigated the changes in RNA expression. The expression levels of differentially expressed (DE) genes and non-coding RNAs (ncRNAs) identified by RNA-seq were validated by quantitative reverse transcription PCR (qRT-PCR). Differential expression was determined using the DESeq R package.Results In total, 207 annotated lncRNAs, 20,552 novel lncRNAs, 3342 novel circRNAs and 19,409 mRNAs were identified. Among these, 70 to 344 DE mRNAs, lncRNAs and circRNAs were detected, and the post-cleavage binding sites between 725 ncRNAs and 2082 miRNAs were predicted. Using the co-location method, we predicted that a total of 235 lncRNAs target 1044 protein-coding genes, while the results of co-expression analysis revealed that 174 lncRNAs target 2097 mRNAs. Pathway enrichment analyses of the genes targeted by ncRNAs suggested that most ncRNAs were significantly enriched in immune or diseases-related pathways. NcRNA regulatory networks revealed that a single ncRNA could be directly or indirectly regulated by multiple genes or ncRNAs that could influence the immune response of cats. Co-expression analysis showed that 242 circRNAs, mainly involved in immune responses, were significantly associated withT. gondii infection. In contrast, 1352 protein coding RNAs, ma
