The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_...The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_(2) and Et_(2)SnCl_(2) can bind to dGMP mainly via phosphate;Cp_(2)TiCl_(2) binds to dGMP mainly via phosphate and N_(7).The binding constant for(CH_(3))_(2)SnCl_(2) binding to phos-phate of dGMP exceeds 2.71×10^(4).The binding constant for Cp_(2)TiCl_(2) to phosphate is even greater than that of Sn(IV).Cis-platin has high affinity for both N_(7) and phosphate,but mainly for N_(7).Binding of the probe to N_(7) of dGMP reduces the binding affinity for phosphate of the same dGMP molecule by a factor of 5 to 6.Much the same factor is expected to apply to other metals containing agents interacting with dGMP.展开更多
基金the National Natural Science Foundation of China(Grant No.20171031)Provincial Natural Foundation of Shanxi.
文摘The interaction of several anti-tumor metal complexes with dGMP have been investigated using trans-[en_(2)Os(h^(2)-H_(2))]^(2+)as a ^(1)H NMR probe in a competitive mode.Me_(2)SnCl_(2),Bu_(2)SnCl_(2),Et_(2)Sn(phen)Cl_(2) and Et_(2)SnCl_(2) can bind to dGMP mainly via phosphate;Cp_(2)TiCl_(2) binds to dGMP mainly via phosphate and N_(7).The binding constant for(CH_(3))_(2)SnCl_(2) binding to phos-phate of dGMP exceeds 2.71×10^(4).The binding constant for Cp_(2)TiCl_(2) to phosphate is even greater than that of Sn(IV).Cis-platin has high affinity for both N_(7) and phosphate,but mainly for N_(7).Binding of the probe to N_(7) of dGMP reduces the binding affinity for phosphate of the same dGMP molecule by a factor of 5 to 6.Much the same factor is expected to apply to other metals containing agents interacting with dGMP.
