From the roots of Panax notoginseng fourteen cyclodipeptides 1-14 were isolated including one new compound (1),seven new natural compounds (4-10) and six known compounds (2-3,11-14) together with one known other compo...From the roots of Panax notoginseng fourteen cyclodipeptides 1-14 were isolated including one new compound (1),seven new natural compounds (4-10) and six known compounds (2-3,11-14) together with one known other compound 15.The chemical structure of 1 was elucidated as cyclo-(Leu-Thr) based on spectral methods.From the roots of Panax ginseng five known lactams (16-20) including pyroglutamic acid were isolated together with butyric diacid,daucosterol and sucrose.The primary bioactivity test showed that pyroglutamic acid and its n-butyl derivative have weak Ca 2+ antagonistic activity.展开更多
The secondary metabolites of the fungus Cephalosporium sp.(2090#) are first reported which isolated from the mangrove sample from the South China Sea.Seven compounds are isolated from the mycelium of this strain.Their...The secondary metabolites of the fungus Cephalosporium sp.(2090#) are first reported which isolated from the mangrove sample from the South China Sea.Seven compounds are isolated from the mycelium of this strain.Their structures are identified by comprehensive spectroscopic methods.展开更多
Cyclodipeptides are diverse chemical scaffolds that show a broad range of bioactivities relevant for medicine,agriculture,chemical catalysis,and material sciences.Cyclodipeptides can be synthesized enzymatically throu...Cyclodipeptides are diverse chemical scaffolds that show a broad range of bioactivities relevant for medicine,agriculture,chemical catalysis,and material sciences.Cyclodipeptides can be synthesized enzymatically through two unrelated enzyme families,non-ribosomal peptide synthetases(NRPS)and cyclodipeptide synthases(CDPSs).The chemical diversity of cyclodipeptides is derived from the two amino acid side chains and the modification of those side-chains by cyclodipeptide tailoring enzymes.While a large spectrum of chemical diversity is already known today,additional chemical space-and as such potential new bioactivities-could be accessed by exploring yet undiscovered NRPS and CDPS gene clusters as well as via engineering.Further,to exploit cyclodipeptides for applications,the low yield of natural biosynthesis needs to be overcome.In this review we summarize current knowledge on NRPS and CDPS-based cyclodipeptide biosynthesis,engineering approaches to further diversity the natural chemical diversity as well as strategies for high-yield production of cyclodipeptides,including a discussion of how advancements in synthetic biology and metabolic engineering can accelerate the translational potential of cyclodipeptides.展开更多
Three novel interesting polychlorinated cyclodipeptides,designated dyssmide A(1),B(2),C(3)and a known compound,2,3-dihydrodyeamide C(4)were isolated from the sponge Dysidea sp.Their structures were elucidated by spect...Three novel interesting polychlorinated cyclodipeptides,designated dyssmide A(1),B(2),C(3)and a known compound,2,3-dihydrodyeamide C(4)were isolated from the sponge Dysidea sp.Their structures were elucidated by spectroscopic methods.展开更多
A mangrove endophytic fungus(No.1947)was isolated from the leaves of Kandelia candel from all estuarine mangrove from the South China Sea.The secondary metabolites of this strain were studied firstly in this paper and...A mangrove endophytic fungus(No.1947)was isolated from the leaves of Kandelia candel from all estuarine mangrove from the South China Sea.The secondary metabolites of this strain were studied firstly in this paper and five compounds were isolated from the fermentation liquid.Their structures were elucidated by spectroscopy methods as three cyclodipeptides,cyclo-(Tyr-Tyr),cyclo-(Leu-Leu)and cyclo-(AIa-Leu),and Uracil and 5-isobutyl-2,4-imidazolidinedione.The results show that this fungal strain can produce abundant azacyclic compounds.展开更多
目的对仿刺参共附生放线菌Kytococcus sp.化学成分进行研究。方法用硅胶柱色谱,Sephadex LH-20凝胶柱色谱,反向高效液相柱层析(RP-HPLC)等分离手段对放线菌(Kytococcus sp.)乙酸乙酯提取物进行分离纯化,并利用1 H NMR、13 C NMR、质谱(...目的对仿刺参共附生放线菌Kytococcus sp.化学成分进行研究。方法用硅胶柱色谱,Sephadex LH-20凝胶柱色谱,反向高效液相柱层析(RP-HPLC)等分离手段对放线菌(Kytococcus sp.)乙酸乙酯提取物进行分离纯化,并利用1 H NMR、13 C NMR、质谱(MS)等手段并与文献对照相结合,鉴定化合物的结构。结果分离得到9个已知的环二肽类化合物,其结构分别为:环(L-脯-L-缬)二肽(1),环(L-脯-L-丙)二肽(2),环(L-脯-L-酪)二肽(3),环(L-脯-L-异亮)二肽(4),环(L-脯-L-亮)二肽(5),环(L-脯-L-苯丙)二肽(6),环(L-缬-L-亮)二肽(7),(L-亮-L-异亮)二肽(8),环(L-亮-L-亮)二肽(9)。结论这是首次从仿刺参中分离得到放线菌Kytococ-cus sp.,9个化合物均为首次从该属放线菌中分离得到。展开更多
文摘From the roots of Panax notoginseng fourteen cyclodipeptides 1-14 were isolated including one new compound (1),seven new natural compounds (4-10) and six known compounds (2-3,11-14) together with one known other compound 15.The chemical structure of 1 was elucidated as cyclo-(Leu-Thr) based on spectral methods.From the roots of Panax ginseng five known lactams (16-20) including pyroglutamic acid were isolated together with butyric diacid,daucosterol and sucrose.The primary bioactivity test showed that pyroglutamic acid and its n-butyl derivative have weak Ca 2+ antagonistic activity.
文摘The secondary metabolites of the fungus Cephalosporium sp.(2090#) are first reported which isolated from the mangrove sample from the South China Sea.Seven compounds are isolated from the mycelium of this strain.Their structures are identified by comprehensive spectroscopic methods.
文摘Cyclodipeptides are diverse chemical scaffolds that show a broad range of bioactivities relevant for medicine,agriculture,chemical catalysis,and material sciences.Cyclodipeptides can be synthesized enzymatically through two unrelated enzyme families,non-ribosomal peptide synthetases(NRPS)and cyclodipeptide synthases(CDPSs).The chemical diversity of cyclodipeptides is derived from the two amino acid side chains and the modification of those side-chains by cyclodipeptide tailoring enzymes.While a large spectrum of chemical diversity is already known today,additional chemical space-and as such potential new bioactivities-could be accessed by exploring yet undiscovered NRPS and CDPS gene clusters as well as via engineering.Further,to exploit cyclodipeptides for applications,the low yield of natural biosynthesis needs to be overcome.In this review we summarize current knowledge on NRPS and CDPS-based cyclodipeptide biosynthesis,engineering approaches to further diversity the natural chemical diversity as well as strategies for high-yield production of cyclodipeptides,including a discussion of how advancements in synthetic biology and metabolic engineering can accelerate the translational potential of cyclodipeptides.
基金Supported by the National Natural Science Foundation the State Education Committee Of China
文摘Three novel interesting polychlorinated cyclodipeptides,designated dyssmide A(1),B(2),C(3)and a known compound,2,3-dihydrodyeamide C(4)were isolated from the sponge Dysidea sp.Their structures were elucidated by spectroscopic methods.
文摘A mangrove endophytic fungus(No.1947)was isolated from the leaves of Kandelia candel from all estuarine mangrove from the South China Sea.The secondary metabolites of this strain were studied firstly in this paper and five compounds were isolated from the fermentation liquid.Their structures were elucidated by spectroscopy methods as three cyclodipeptides,cyclo-(Tyr-Tyr),cyclo-(Leu-Leu)and cyclo-(AIa-Leu),and Uracil and 5-isobutyl-2,4-imidazolidinedione.The results show that this fungal strain can produce abundant azacyclic compounds.
文摘目的对仿刺参共附生放线菌Kytococcus sp.化学成分进行研究。方法用硅胶柱色谱,Sephadex LH-20凝胶柱色谱,反向高效液相柱层析(RP-HPLC)等分离手段对放线菌(Kytococcus sp.)乙酸乙酯提取物进行分离纯化,并利用1 H NMR、13 C NMR、质谱(MS)等手段并与文献对照相结合,鉴定化合物的结构。结果分离得到9个已知的环二肽类化合物,其结构分别为:环(L-脯-L-缬)二肽(1),环(L-脯-L-丙)二肽(2),环(L-脯-L-酪)二肽(3),环(L-脯-L-异亮)二肽(4),环(L-脯-L-亮)二肽(5),环(L-脯-L-苯丙)二肽(6),环(L-缬-L-亮)二肽(7),(L-亮-L-异亮)二肽(8),环(L-亮-L-亮)二肽(9)。结论这是首次从仿刺参中分离得到放线菌Kytococ-cus sp.,9个化合物均为首次从该属放线菌中分离得到。
