以道地产地高良姜为材料,对该药用植物内生细菌种群组成、组织分布及其促生潜力进行了研究。采用组织块分离法,从高良姜根、根茎、茎和叶4个组织共计分离得到细菌136株,分属于16个16S r DNA基因型、12个细菌属、15个细菌种,其中,芽孢杆...以道地产地高良姜为材料,对该药用植物内生细菌种群组成、组织分布及其促生潜力进行了研究。采用组织块分离法,从高良姜根、根茎、茎和叶4个组织共计分离得到细菌136株,分属于16个16S r DNA基因型、12个细菌属、15个细菌种,其中,芽孢杆菌、甲基杆菌分别为其最、次优势种群。各种群分离比随组织不同而异;采用Hha I消化的末端限制性片段多态性(TRFLP)免培养方法,从高良姜4个组织共计检测到36个不同的末端限制性酶切片段(T-RFs)。种群对应分析表明其免培养内生细菌群体主要包括芽孢杆菌、甲基杆菌等高抗性细菌,海洋螺菌、红杆菌、交替假单胞菌等海洋细菌及热带根瘤菌、沼泽考克氏菌等热带相关细菌,说明高良姜内生细菌群体与其宿主植物生长环境密切相关。不同组织其总T-RFs数目、优势T-RFs及其对应细菌种群明显不同;在所分离的细菌菌株中,36.36%、51.52%、54.55%和27.27%的菌株分别显示了胞外几丁质酶、β-葡聚糖酶,生长素及其1-氨基环丙烷-1-羧酸(ACC)脱氨酶产生能力。其中,泛菌L-2、芽孢杆菌S-16所测4个指标均为阳性,热带根瘤菌菌株R-1和R-3显示了较高的ACC脱氨酶及生长素产生能力,这些菌株是促生菌剂的良好候选。展开更多
Zha-chili is a naturally fermented traditional food from central southern China.Corn-based zha-chili is one of the most popular varieties with a special flavor.To investigate its distinct microbiome,corn-based zha-chi...Zha-chili is a naturally fermented traditional food from central southern China.Corn-based zha-chili is one of the most popular varieties with a special flavor.To investigate its distinct microbiome,corn-based zha-chili samples were collected and investigated using both high-throughput sequencing and culture-dependent method.Subsequently,E-nose and E-tongue sensors were used to evaluate the overall sensory profile.We found that the bacterial communities present in zha-chili samples from the two regions were significantly different(P<0.05).Companilactobacillus,Lactiplantibacillus,and Corynebacterium were found to be the dominant genera common to samples from both regions.Among these,lactic acid bacteria were the most dominant.Biomarker genera were Ligilactobacillus,Lactobacillus,and Levilactobacillus(Huaihua)and Pantoea,Lactiplantibacillus,and Weissella(Songtao).Using culture-dependent methods,the most dominant genus Companilactobacillus was found to comprise Companilactobacillus alimentarius and Companilactobacillus futsaii.The sensory profile was also characterized.This indicated that zha-chili from the two regions could be discriminated by sourness and aftertaste-B(E-taste),and W1C,W3C,W5C,W1S,W2S,W5S,and W2W(E-nose).Correlation analysis suggested that the dominant genera might not affect the aroma quality of corn-based zha-chili,whereas they were related to taste quality.Levilactobacillus and Ligilactobacillus were positively correlated with the characteristic taste indices and negatively correlated with the off-flavor indices.Another dominant genus,Kocuria was positively correlated with umami and richness(P<0.05).Our results could provide a basis for supporting zha-chili production and help the isolation of appropriate lactic acid bacteria from corn-based zha-chili.展开更多
为了解麦洼牦牛肠道细菌群落结构组成和功能及其动态变化过程,同时获得具有潜在益生菌活性和应用可能性的肠道菌株,采用不同的培养基在不同的培养条件下分离麦洼牦牛肠道细菌并进行16S r DNA分子鉴定,同时采用变性梯度凝胶电泳(denaturi...为了解麦洼牦牛肠道细菌群落结构组成和功能及其动态变化过程,同时获得具有潜在益生菌活性和应用可能性的肠道菌株,采用不同的培养基在不同的培养条件下分离麦洼牦牛肠道细菌并进行16S r DNA分子鉴定,同时采用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术分析不同年龄麦洼牦牛肠道细菌的群落结构和功能及其差异。结果表明,从不同年龄麦洼牦牛大肠内容物中共分离到90株细菌,其中3株归为放线菌门(Actinobacteria),64株为厚壁菌门(Firmicutes),23株为变形菌门(Proteobacteria);分离到的34株芽孢杆菌属(Bacillus)和14株乳酸杆菌属(Lactobacillus)细菌具有潜在益生菌活性,可作为微生物饲料添加剂补饲麦洼牦牛;对DGGE图谱的UPGMA聚类、PCA和多样性指数分析显示,不同年龄的麦洼牦牛肠道细菌的群落结构有差异,相同年龄的个体相似度较高,而不同年龄的个体相似度较小,较大年龄(2.5和3.5岁)的牦牛肠道菌群多样性大于较小年龄个体(0.5和1.5岁),且分布更均匀,肠道菌群随宿主年龄变化是一个动态变化并趋于稳定的过程;DGGE条带的物种鉴定结果表明,大部分条带为未(能)培养细菌,PCR-DGGE与分离培养法结合可以很好地表征麦洼牦牛肠道菌群的结构和功能。展开更多
应用PCR—DGGE技术对某微生物肥料的质量进行了跟踪监测，并结合分离培养和克隆文库分析对其菌种组成进行了检测。结果表明，同一生产批次3个不同包装样品的细菌和真菌DGGE（denaturing gradient gel eleetrophoresis）图谱相似性为80％...应用PCR—DGGE技术对某微生物肥料的质量进行了跟踪监测，并结合分离培养和克隆文库分析对其菌种组成进行了检测。结果表明，同一生产批次3个不同包装样品的细菌和真菌DGGE（denaturing gradient gel eleetrophoresis）图谱相似性为80％-100％，3个不同生产批次之间DGGE图谱相似性为80％-88％，表明该微生物肥料的菌种组成的稳定性较好。但分离培养和克隆文库分析结果显示样品的菌种组成与产品标签说明之间存在较大差异。对于产品标注的6种微生物组成，只有Lactobacillus属的微生物可与之对应，其他检测到的Bacillus、Monascus、Brevibacillus、Psudomonas和Penicillium属的微生物并未包括在产品说明中。研究表明用分子生态学方法可以比较客观准确的对微生物肥料质量进行评估和峪测。展开更多
文摘以道地产地高良姜为材料,对该药用植物内生细菌种群组成、组织分布及其促生潜力进行了研究。采用组织块分离法,从高良姜根、根茎、茎和叶4个组织共计分离得到细菌136株,分属于16个16S r DNA基因型、12个细菌属、15个细菌种,其中,芽孢杆菌、甲基杆菌分别为其最、次优势种群。各种群分离比随组织不同而异;采用Hha I消化的末端限制性片段多态性(TRFLP)免培养方法,从高良姜4个组织共计检测到36个不同的末端限制性酶切片段(T-RFs)。种群对应分析表明其免培养内生细菌群体主要包括芽孢杆菌、甲基杆菌等高抗性细菌,海洋螺菌、红杆菌、交替假单胞菌等海洋细菌及热带根瘤菌、沼泽考克氏菌等热带相关细菌,说明高良姜内生细菌群体与其宿主植物生长环境密切相关。不同组织其总T-RFs数目、优势T-RFs及其对应细菌种群明显不同;在所分离的细菌菌株中,36.36%、51.52%、54.55%和27.27%的菌株分别显示了胞外几丁质酶、β-葡聚糖酶,生长素及其1-氨基环丙烷-1-羧酸(ACC)脱氨酶产生能力。其中,泛菌L-2、芽孢杆菌S-16所测4个指标均为阳性,热带根瘤菌菌株R-1和R-3显示了较高的ACC脱氨酶及生长素产生能力,这些菌株是促生菌剂的良好候选。
基金This research is supported by Key projects of science and technology research program of Hubei Provincial Department of Education(D20212601).
文摘Zha-chili is a naturally fermented traditional food from central southern China.Corn-based zha-chili is one of the most popular varieties with a special flavor.To investigate its distinct microbiome,corn-based zha-chili samples were collected and investigated using both high-throughput sequencing and culture-dependent method.Subsequently,E-nose and E-tongue sensors were used to evaluate the overall sensory profile.We found that the bacterial communities present in zha-chili samples from the two regions were significantly different(P<0.05).Companilactobacillus,Lactiplantibacillus,and Corynebacterium were found to be the dominant genera common to samples from both regions.Among these,lactic acid bacteria were the most dominant.Biomarker genera were Ligilactobacillus,Lactobacillus,and Levilactobacillus(Huaihua)and Pantoea,Lactiplantibacillus,and Weissella(Songtao).Using culture-dependent methods,the most dominant genus Companilactobacillus was found to comprise Companilactobacillus alimentarius and Companilactobacillus futsaii.The sensory profile was also characterized.This indicated that zha-chili from the two regions could be discriminated by sourness and aftertaste-B(E-taste),and W1C,W3C,W5C,W1S,W2S,W5S,and W2W(E-nose).Correlation analysis suggested that the dominant genera might not affect the aroma quality of corn-based zha-chili,whereas they were related to taste quality.Levilactobacillus and Ligilactobacillus were positively correlated with the characteristic taste indices and negatively correlated with the off-flavor indices.Another dominant genus,Kocuria was positively correlated with umami and richness(P<0.05).Our results could provide a basis for supporting zha-chili production and help the isolation of appropriate lactic acid bacteria from corn-based zha-chili.
文摘为了解麦洼牦牛肠道细菌群落结构组成和功能及其动态变化过程,同时获得具有潜在益生菌活性和应用可能性的肠道菌株,采用不同的培养基在不同的培养条件下分离麦洼牦牛肠道细菌并进行16S r DNA分子鉴定,同时采用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术分析不同年龄麦洼牦牛肠道细菌的群落结构和功能及其差异。结果表明,从不同年龄麦洼牦牛大肠内容物中共分离到90株细菌,其中3株归为放线菌门(Actinobacteria),64株为厚壁菌门(Firmicutes),23株为变形菌门(Proteobacteria);分离到的34株芽孢杆菌属(Bacillus)和14株乳酸杆菌属(Lactobacillus)细菌具有潜在益生菌活性,可作为微生物饲料添加剂补饲麦洼牦牛;对DGGE图谱的UPGMA聚类、PCA和多样性指数分析显示,不同年龄的麦洼牦牛肠道细菌的群落结构有差异,相同年龄的个体相似度较高,而不同年龄的个体相似度较小,较大年龄(2.5和3.5岁)的牦牛肠道菌群多样性大于较小年龄个体(0.5和1.5岁),且分布更均匀,肠道菌群随宿主年龄变化是一个动态变化并趋于稳定的过程;DGGE条带的物种鉴定结果表明,大部分条带为未(能)培养细菌,PCR-DGGE与分离培养法结合可以很好地表征麦洼牦牛肠道菌群的结构和功能。
文摘应用PCR—DGGE技术对某微生物肥料的质量进行了跟踪监测，并结合分离培养和克隆文库分析对其菌种组成进行了检测。结果表明，同一生产批次3个不同包装样品的细菌和真菌DGGE（denaturing gradient gel eleetrophoresis）图谱相似性为80％-100％，3个不同生产批次之间DGGE图谱相似性为80％-88％，表明该微生物肥料的菌种组成的稳定性较好。但分离培养和克隆文库分析结果显示样品的菌种组成与产品标签说明之间存在较大差异。对于产品标注的6种微生物组成，只有Lactobacillus属的微生物可与之对应，其他检测到的Bacillus、Monascus、Brevibacillus、Psudomonas和Penicillium属的微生物并未包括在产品说明中。研究表明用分子生态学方法可以比较客观准确的对微生物肥料质量进行评估和峪测。
