Diabetes mellitus control in Mexico is practiced by using antidiabetic agents and, by empirical way using medicinal plants. Cucurbita ficifolia (C. ficifolia) has been attributed with hypoglycemic, hypotriglyceridemic...Diabetes mellitus control in Mexico is practiced by using antidiabetic agents and, by empirical way using medicinal plants. Cucurbita ficifolia (C. ficifolia) has been attributed with hypoglycemic, hypotriglyceridemic, and anti-inflam- matory effects, and D-chiro-inositol (DCI), 4-hydroxybenzoic acid, and β-sitosterol were proposed as active principles. The last two compounds were suggested as activators of two transcription factors, PPARα and PPARγ, in C2C12 myocytes, which participate in β-oxidation of fatty acids and insulin sensitivity. However, the involvement of the hepatocytic and adipocytic PPARs in the effects of C. ficifolia has not yet been explored. This research aimed to determine the effects of C. ficifolia on PPARα, PPARγ, and inflammatory cytokines in streptozotocin (STZ)-induced diabetes mice, HepG2 hepatocytes and 3T3-L1 adipocytes, implicating two additional cell types associated with metabolism of carbohydrates and lipids. STZ-induced diabetes mice received C. ficifolia (200 mg/kg/day) for 30 days, measuring serum cytokines (TNF-α and IL-6). RNA was extracted from liver. Besides, HepG2 and 3T3-L1 cells were incubated (24 h) with C. ficifolia (0.078 mM DCI), using pioglitazone or fenofibrate as controls. RNA was also extracted from cells and PCR in real-time was performed to determinate PPARα and PPARγ expression. In diabetic animals, C. ficifolia decreased glycemia and body weight, decreasing the expression level of TNF-α and IL-6. In addition, C. ficifolia increased PPARα expression in liver of diabetic animals, in HepG2 and 3T3-L1 cells;PPARγ expression only significantly increased in HepG2 cells. The data suggest that the effects on the glycemia and lipids of C. ficifolia and its anti-inflammatory effects imply, besides skeletal muscle cells, hepatic and adipocytic PPARα activation, without affectation on PPARγ. PPARs regulation by C. ficifolia may improve the metabolic dysfunctions associated with metabolic disease, controlling the intake, activation, and oxidation of fatty a展开更多
用不同浓度的N aC l胁迫处理黑籽南瓜和西葫芦种子,观察其发芽情况,计算发芽率、发芽势、发芽指数和活力指数,进行对比分析。结果表明,黑籽南瓜种子萌发对盐水生境的适应性比西葫芦强,黑籽南瓜种子萌发的N aC l胁迫浓度适宜值为50.66 mm...用不同浓度的N aC l胁迫处理黑籽南瓜和西葫芦种子,观察其发芽情况,计算发芽率、发芽势、发芽指数和活力指数,进行对比分析。结果表明,黑籽南瓜种子萌发对盐水生境的适应性比西葫芦强,黑籽南瓜种子萌发的N aC l胁迫浓度适宜值为50.66 mm o l/L,而西葫芦为43.98 mm o l/L。展开更多
Polysaccharides obtained from plant have been investigated for the development of edible/biodegradable non petrochemical-based packaging materials. Alcayota (Curcubita ficifolia) is the fruit of a creeping plant such ...Polysaccharides obtained from plant have been investigated for the development of edible/biodegradable non petrochemical-based packaging materials. Alcayota (Curcubita ficifolia) is the fruit of a creeping plant such as watermelon and melon. It is the fruit of a creeping plant such as watermelon and melon. After separating the pulp from the husk and seeds it is dried and ground to obtain a flour. Different alcayota flour is made to hydrolyze then. Alkaline hydrolysis is released to extraction of alcayota gum and is purified by hydroalcoholic solutions. The films were prepared from water solution of hydrolyzed alcayota gum (AlcOH). AlcOH film present properties are mainly due to the strong hydrophilicity. In order to improve water resistance, the films were modified using glutaraldehyde (Glu), in order to make the water insoluble film. The crosslinked films providing a low water vapor permeability (WVP) and high mechanical properties expressed in elastic modulus. The X-ray diffraction showed amorphous and shift to lower dspacing, i.e. at lower distances between the polysaccharide chains. These crosslinked membranes exhibit excellent water resistance, low O2 permeation, which make them very useful in selecting biodegradable polymer and films.展开更多
文摘Diabetes mellitus control in Mexico is practiced by using antidiabetic agents and, by empirical way using medicinal plants. Cucurbita ficifolia (C. ficifolia) has been attributed with hypoglycemic, hypotriglyceridemic, and anti-inflam- matory effects, and D-chiro-inositol (DCI), 4-hydroxybenzoic acid, and β-sitosterol were proposed as active principles. The last two compounds were suggested as activators of two transcription factors, PPARα and PPARγ, in C2C12 myocytes, which participate in β-oxidation of fatty acids and insulin sensitivity. However, the involvement of the hepatocytic and adipocytic PPARs in the effects of C. ficifolia has not yet been explored. This research aimed to determine the effects of C. ficifolia on PPARα, PPARγ, and inflammatory cytokines in streptozotocin (STZ)-induced diabetes mice, HepG2 hepatocytes and 3T3-L1 adipocytes, implicating two additional cell types associated with metabolism of carbohydrates and lipids. STZ-induced diabetes mice received C. ficifolia (200 mg/kg/day) for 30 days, measuring serum cytokines (TNF-α and IL-6). RNA was extracted from liver. Besides, HepG2 and 3T3-L1 cells were incubated (24 h) with C. ficifolia (0.078 mM DCI), using pioglitazone or fenofibrate as controls. RNA was also extracted from cells and PCR in real-time was performed to determinate PPARα and PPARγ expression. In diabetic animals, C. ficifolia decreased glycemia and body weight, decreasing the expression level of TNF-α and IL-6. In addition, C. ficifolia increased PPARα expression in liver of diabetic animals, in HepG2 and 3T3-L1 cells;PPARγ expression only significantly increased in HepG2 cells. The data suggest that the effects on the glycemia and lipids of C. ficifolia and its anti-inflammatory effects imply, besides skeletal muscle cells, hepatic and adipocytic PPARα activation, without affectation on PPARγ. PPARs regulation by C. ficifolia may improve the metabolic dysfunctions associated with metabolic disease, controlling the intake, activation, and oxidation of fatty a
文摘Polysaccharides obtained from plant have been investigated for the development of edible/biodegradable non petrochemical-based packaging materials. Alcayota (Curcubita ficifolia) is the fruit of a creeping plant such as watermelon and melon. It is the fruit of a creeping plant such as watermelon and melon. After separating the pulp from the husk and seeds it is dried and ground to obtain a flour. Different alcayota flour is made to hydrolyze then. Alkaline hydrolysis is released to extraction of alcayota gum and is purified by hydroalcoholic solutions. The films were prepared from water solution of hydrolyzed alcayota gum (AlcOH). AlcOH film present properties are mainly due to the strong hydrophilicity. In order to improve water resistance, the films were modified using glutaraldehyde (Glu), in order to make the water insoluble film. The crosslinked films providing a low water vapor permeability (WVP) and high mechanical properties expressed in elastic modulus. The X-ray diffraction showed amorphous and shift to lower dspacing, i.e. at lower distances between the polysaccharide chains. These crosslinked membranes exhibit excellent water resistance, low O2 permeation, which make them very useful in selecting biodegradable polymer and films.
