Although auxin and brassinosteroid （BR） synergistically control various plant responses, the molecular mechanism underlying the auxin-BR crosstalk is not wen understood. We previously identified SMOS1, an auxin-regu...Although auxin and brassinosteroid （BR） synergistically control various plant responses, the molecular mechanism underlying the auxin-BR crosstalk is not wen understood. We previously identified SMOS1, an auxin-regulated APETALA2-type transcription factor, as the causal gene of the small organ size 1 （smosl） mutant that is characterized by a decreased final size of various organs in rice. In this study, we identified another smos mutant, smos2, which shows the phenotype indistinguishable from smosl. SMOS2 was identical to the previously reported DWARF AND LOWoTILLERING （DLT）, which encodes a GRAS protein involved in BR signaling. SMOS1 and SMOS2/DLT physically interact to cooperatively enhance transcriptional transactivation activity in yeast and in rice nuclei. Consistently, the expression of OsPHI-1, a direct target of SMOS1, is upregulated only when SMOS1 and SMOS2/DLT proteins are both present in rice ceils. Taken together, our results suggest that SMOS1 and SMOS2/DLT form a keystone complex on auxin-BR signaling crosstalk in rice.展开更多
The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective respon...The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive element.However,it has become increasingly clear from our works and others that nuclear receptors are important regulators of cytokine production and function through complex and varied interactions between these distinct transcriptional factors.This review provides a general overview of the mechanism of action of nuclear receptors and their transcriptional crosstalk with transcriptional factors associated with cytokine transduction pathways.One of the most important mechanistic aspects is protein to protein interaction through a direct or co-regulator-mediated indirect manner.Such crosstalk is crucially involved in physiological and therapeutic roles of nuclear receptors and their iigands in immunity, inflammation and cytokine-related tumors.Cellular & Molecular Immunology.2004;1(6):416-424.展开更多
Preterm labor(before 37 weeks’gestation)is the leading cause of neonatal mortality and morbidity,which can be divided into iatrogenic preterm labor,infectious preterm labor,and spontaneous preterm labor(sPTL).Up to n...Preterm labor(before 37 weeks’gestation)is the leading cause of neonatal mortality and morbidity,which can be divided into iatrogenic preterm labor,infectious preterm labor,and spontaneous preterm labor(sPTL).Up to now,there continue to be great difficulties in prediction and prevention of sPTL,owing to multiple risk factors,pathogenesis,and pathologic processes contributing to the event,which have not been fully clarified.Pregnancy maintenance and parturition is a complicated process with continuous maternal-fetal dialogue,in which both maternal and fetal factors participate and affect the outcome of pregnancy,including sPTL.Besides,external factors can also participate in sPTL,individually or through the interaction with internal factors.In this article,we summarize recent studies regarding sPTL from our and other groups,and discuss the risk factors and pathogenesis of preterm birth from both external and internal(maternal and fetal)aspects,so as to provide theoretical evidences for the diagnosis,prevention,and treatment of sPTL in the future.展开更多
Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzhe...Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzheimer's disease(AD)mice.Blood brain barrier injury may also have contributed to this increase.Immune regulation by microglia plays a major role in the progression of aging and AD,according to the functions of 41 intersecting differentially expressed genes in microglia.Signaling crosstalk between C−C motif chemokine ligand(CCL)and major histocompatibility complex-1 bridges intercellular communication in the hippocampus during aging and AD.The amyloid precursor protein(APP)and colony stimulating factor(CSF)signals drive 5×FAD to deviate from aging track to AD occurrence among intercellular communication in hippocampus.Microglia are involved in the progression of aging and AD can be divided into 10 functional types.The strength of the interaction among microglial subtypes weakened with aging,and the CCL and CSF signaling pathways were the fundamental bridge of communication among microglial subtypes.展开更多
为应对3He短缺情况,采用更加低价、易获取的液闪来探测中子,并推广应用中子多重性技术,研制了一种基于EJ309的液闪快中子探测器,自主开发了中子伽玛甄别电路,采用电荷积分法进行中子伽玛信号甄别,并通过实验验证了该中子探测器在能量阈...为应对3He短缺情况,采用更加低价、易获取的液闪来探测中子,并推广应用中子多重性技术,研制了一种基于EJ309的液闪快中子探测器,自主开发了中子伽玛甄别电路,采用电荷积分法进行中子伽玛信号甄别,并通过实验验证了该中子探测器在能量阈值为100 keVee(ee—等效能)时,中子伽玛甄别性能参数FOM(Figure of Merit)大于1;在多重性数据分析过程中,为了解决散射中子对多重性符合计数带来的影响,引入散射串扰因子对传统多重性方程进行了修正;为降低测量腔内探测效率的非均匀性对测量结果的影响,通过蒙特卡洛模拟设计了一套球形快中子多重性测量装置,并通过实验验证了其探测效率的均匀性以及测量结果的相对偏差,结果表明:其探测效率的均匀性以及测量结果的相对偏差均好于5%,该工作为快中子多重性测量技术的实际应用奠定了基础。展开更多
Bone has long been considered as a silent organ that provides a reservoir of calcium and phosphorus,traditionally.Recently,further study of bone has revealed additional functions as an endocrine organ connecting syste...Bone has long been considered as a silent organ that provides a reservoir of calcium and phosphorus,traditionally.Recently,further study of bone has revealed additional functions as an endocrine organ connecting systemic organs of the whole body.Communication between bone and other organs participates in most physiological and pathological events and is responsible for the maintenance of homeostasis.Here,we present an overview of the crosstalk between bone and other organs.Furthermore,we describe the factors mediating the cross-talk and review the mechanisms in the development of potential associated diseases.These connections shed new light on the pathogenesis of systemic diseases and provide novel potential targets for the treatment of systemic diseases.展开更多
文摘Although auxin and brassinosteroid （BR） synergistically control various plant responses, the molecular mechanism underlying the auxin-BR crosstalk is not wen understood. We previously identified SMOS1, an auxin-regulated APETALA2-type transcription factor, as the causal gene of the small organ size 1 （smosl） mutant that is characterized by a decreased final size of various organs in rice. In this study, we identified another smos mutant, smos2, which shows the phenotype indistinguishable from smosl. SMOS2 was identical to the previously reported DWARF AND LOWoTILLERING （DLT）, which encodes a GRAS protein involved in BR signaling. SMOS1 and SMOS2/DLT physically interact to cooperatively enhance transcriptional transactivation activity in yeast and in rice nuclei. Consistently, the expression of OsPHI-1, a direct target of SMOS1, is upregulated only when SMOS1 and SMOS2/DLT proteins are both present in rice ceils. Taken together, our results suggest that SMOS1 and SMOS2/DLT form a keystone complex on auxin-BR signaling crosstalk in rice.
文摘The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive element.However,it has become increasingly clear from our works and others that nuclear receptors are important regulators of cytokine production and function through complex and varied interactions between these distinct transcriptional factors.This review provides a general overview of the mechanism of action of nuclear receptors and their transcriptional crosstalk with transcriptional factors associated with cytokine transduction pathways.One of the most important mechanistic aspects is protein to protein interaction through a direct or co-regulator-mediated indirect manner.Such crosstalk is crucially involved in physiological and therapeutic roles of nuclear receptors and their iigands in immunity, inflammation and cytokine-related tumors.Cellular & Molecular Immunology.2004;1(6):416-424.
基金supported by National Key Research and Development Project No.2019YFC1005203National Natural Science Foundation of China No.81771608 and 82120108011+2 种基金Major Project of Shanghai Municipal Education Commission’s Scientific Research and Innovation Plan No.2021-01-07-00-07-E00144“Dawn”Program of Shanghai Education Commission No.17SG36The Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning.
文摘Preterm labor(before 37 weeks’gestation)is the leading cause of neonatal mortality and morbidity,which can be divided into iatrogenic preterm labor,infectious preterm labor,and spontaneous preterm labor(sPTL).Up to now,there continue to be great difficulties in prediction and prevention of sPTL,owing to multiple risk factors,pathogenesis,and pathologic processes contributing to the event,which have not been fully clarified.Pregnancy maintenance and parturition is a complicated process with continuous maternal-fetal dialogue,in which both maternal and fetal factors participate and affect the outcome of pregnancy,including sPTL.Besides,external factors can also participate in sPTL,individually or through the interaction with internal factors.In this article,we summarize recent studies regarding sPTL from our and other groups,and discuss the risk factors and pathogenesis of preterm birth from both external and internal(maternal and fetal)aspects,so as to provide theoretical evidences for the diagnosis,prevention,and treatment of sPTL in the future.
基金supported by grants from the Key R&D Plan of the Science and Technology Plan of Tibet Autonomous Region,China(Grant No.:XZ202201ZY0026G)the Science and Technology Cooperation Project of Shandong Provincial Department of Science and Technology for Counterpart Assistance to Tibet,China(Grant No.:YDZX2021083)+1 种基金the National Natural Science Foundation of China(Grant No.:82205078)the Natural Science Foundation of Shandong Province,China(Grant No.:ZR2021QH157).
文摘Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzheimer's disease(AD)mice.Blood brain barrier injury may also have contributed to this increase.Immune regulation by microglia plays a major role in the progression of aging and AD,according to the functions of 41 intersecting differentially expressed genes in microglia.Signaling crosstalk between C−C motif chemokine ligand(CCL)and major histocompatibility complex-1 bridges intercellular communication in the hippocampus during aging and AD.The amyloid precursor protein(APP)and colony stimulating factor(CSF)signals drive 5×FAD to deviate from aging track to AD occurrence among intercellular communication in hippocampus.Microglia are involved in the progression of aging and AD can be divided into 10 functional types.The strength of the interaction among microglial subtypes weakened with aging,and the CCL and CSF signaling pathways were the fundamental bridge of communication among microglial subtypes.
文摘为应对3He短缺情况,采用更加低价、易获取的液闪来探测中子,并推广应用中子多重性技术,研制了一种基于EJ309的液闪快中子探测器,自主开发了中子伽玛甄别电路,采用电荷积分法进行中子伽玛信号甄别,并通过实验验证了该中子探测器在能量阈值为100 keVee(ee—等效能)时,中子伽玛甄别性能参数FOM(Figure of Merit)大于1;在多重性数据分析过程中,为了解决散射中子对多重性符合计数带来的影响,引入散射串扰因子对传统多重性方程进行了修正;为降低测量腔内探测效率的非均匀性对测量结果的影响,通过蒙特卡洛模拟设计了一套球形快中子多重性测量装置,并通过实验验证了其探测效率的均匀性以及测量结果的相对偏差,结果表明:其探测效率的均匀性以及测量结果的相对偏差均好于5%,该工作为快中子多重性测量技术的实际应用奠定了基础。
基金supported by the National key research and development program(2020YFC2009004,2021YFC2501700)National Natural Science Foundation of China(81874010)and PKU-Baidu Fund(2020BD014).
文摘Bone has long been considered as a silent organ that provides a reservoir of calcium and phosphorus,traditionally.Recently,further study of bone has revealed additional functions as an endocrine organ connecting systemic organs of the whole body.Communication between bone and other organs participates in most physiological and pathological events and is responsible for the maintenance of homeostasis.Here,we present an overview of the crosstalk between bone and other organs.Furthermore,we describe the factors mediating the cross-talk and review the mechanisms in the development of potential associated diseases.These connections shed new light on the pathogenesis of systemic diseases and provide novel potential targets for the treatment of systemic diseases.
