Although anti-cancer nanotherapeutics have made breakthroughs,many remain clinically unsatisfactory due to limited delivery efficiency and complicated biological barriers.Here,we prepared charge-reversible crosslinked...Although anti-cancer nanotherapeutics have made breakthroughs,many remain clinically unsatisfactory due to limited delivery efficiency and complicated biological barriers.Here,we prepared charge-reversible crosslinked nanoparticles(PDC NPs)by supramolecular self-assembly of pro-apoptotic peptides and photosensitizers,followed by crosslinking the self-assemblies with polyethylene glycol to impart tumor microenvironment responsiveness and charge-reversibility.The resultant PDC NPs have a high drug loading of 68.3%,substantially exceeding that of 10%–15%in conventional drug delivery systems.PDC NPs can overcome the delivery hurdles to significantly improve the tumor accumulation and endocytosis of payloads by surface charge reversal and responsive crosslinking strategy.Pro-apoptotic peptides target the mitochondrial membranes and block the respiratory effect to reduce local oxygen consumption,which extensively augments oxygen-dependent photodynamic therapy(PDT).The photosensitizers around mitochondria increased along with the peptides,allowing PDT to work with pro-apoptotic peptides synergistically to induce tumor cell death by mitochondria-dependent apoptotic pathways.Our strategy would provide a valuable reference for improving the delivery efficiency of hydrophilic peptides and developing mitochondrial-targeting cancer therapies.展开更多
This short review is dedicated to celebrate Prof.Shoukuan Fu’s 80 th birthday by discussing several of my accomplished projects over the past twenty years,which all applied radical polymerization in aqueous dispersed...This short review is dedicated to celebrate Prof.Shoukuan Fu’s 80 th birthday by discussing several of my accomplished projects over the past twenty years,which all applied radical polymerization in aqueous dispersed media for producing polymers with branched structures.These projects include the use of microemulsion polymerization for syntheses of fluorescent nanoparticles,hairy nanoparticles and hyperbranched polymers;the use of miniemulsion polymerization for synthesis of star polymers and light-emitting nanoparticles;the use of seeded emulsion polymerization for synthesis of hairy nanoparticles and hyperstar polymers;and the use of precipitation polymerization for synthesis of hollow polymer nanocapsules.Discussion of these projects demonstrates intriguing features of polymerization in biphasic dispersed media via either conventional radical polymerization or controlled radical polymerization to effectively regulate the branched structure of functional polymers.展开更多
基金support from the National Natural Science Foundation of China(Nos.82172084 and 81803002)STI2030-Major Projects(No.2022ZD0212500)。
文摘Although anti-cancer nanotherapeutics have made breakthroughs,many remain clinically unsatisfactory due to limited delivery efficiency and complicated biological barriers.Here,we prepared charge-reversible crosslinked nanoparticles(PDC NPs)by supramolecular self-assembly of pro-apoptotic peptides and photosensitizers,followed by crosslinking the self-assemblies with polyethylene glycol to impart tumor microenvironment responsiveness and charge-reversibility.The resultant PDC NPs have a high drug loading of 68.3%,substantially exceeding that of 10%–15%in conventional drug delivery systems.PDC NPs can overcome the delivery hurdles to significantly improve the tumor accumulation and endocytosis of payloads by surface charge reversal and responsive crosslinking strategy.Pro-apoptotic peptides target the mitochondrial membranes and block the respiratory effect to reduce local oxygen consumption,which extensively augments oxygen-dependent photodynamic therapy(PDT).The photosensitizers around mitochondria increased along with the peptides,allowing PDT to work with pro-apoptotic peptides synergistically to induce tumor cell death by mitochondria-dependent apoptotic pathways.Our strategy would provide a valuable reference for improving the delivery efficiency of hydrophilic peptides and developing mitochondrial-targeting cancer therapies.
基金the University of Notre Dame for financial support
文摘This short review is dedicated to celebrate Prof.Shoukuan Fu’s 80 th birthday by discussing several of my accomplished projects over the past twenty years,which all applied radical polymerization in aqueous dispersed media for producing polymers with branched structures.These projects include the use of microemulsion polymerization for syntheses of fluorescent nanoparticles,hairy nanoparticles and hyperbranched polymers;the use of miniemulsion polymerization for synthesis of star polymers and light-emitting nanoparticles;the use of seeded emulsion polymerization for synthesis of hairy nanoparticles and hyperstar polymers;and the use of precipitation polymerization for synthesis of hollow polymer nanocapsules.Discussion of these projects demonstrates intriguing features of polymerization in biphasic dispersed media via either conventional radical polymerization or controlled radical polymerization to effectively regulate the branched structure of functional polymers.
