Unique coupling reagent, bis-(2-hydroxyethyl methacrylate) phosphate was used to prepare coated and functionalized superparamagnetic nanobeads, leading to a simple, effective method for coating the nanobeads. With th...Unique coupling reagent, bis-(2-hydroxyethyl methacrylate) phosphate was used to prepare coated and functionalized superparamagnetic nanobeads, leading to a simple, effective method for coating the nanobeads. With this method, the thickness of the coating layer and the functional group contents on the nano-beads could be controlled by changing the quantity of the coated monomers. The nanobeads were characterized by means of transmission electron microscopy (TEM) and Fourier transformation infrared spectroscopy (FTIR). The carboxyl-modified magnetic nano-beads were employed to streamline the protocol of isolation of genomic DNA from the human whole blood.展开更多
We describe a convenient method for the synthesis of 1,2-disubstituted acetylenes via a cross-coupling reaction of （bromoethynyl）benzene with Grignard reagents. The reaction of （bromoethynyl）benzene （1 mmol） wit...We describe a convenient method for the synthesis of 1,2-disubstituted acetylenes via a cross-coupling reaction of （bromoethynyl）benzene with Grignard reagents. The reaction of （bromoethynyl）benzene （1 mmol） with Grignard reagent （1.3 mmol） mediated by NiCl2 （4 mol%） and （p-CH3Ph）3P （8 mol%） in THF could produce 1,2-disubstituted acetylenes in good yields at room temperature.展开更多
1,3-Bis(2,6-diisopropylphenyl)imidazolium chloride, [DIPrim]Cl, was used to produce a novel iron(III)-containing imidazolium salt [DIPrim][FeCl 4 ], which included a N,N-diarylimidazolium cation (R = 2,6-diisopropylph...1,3-Bis(2,6-diisopropylphenyl)imidazolium chloride, [DIPrim]Cl, was used to produce a novel iron(III)-containing imidazolium salt [DIPrim][FeCl 4 ], which included a N,N-diarylimidazolium cation (R = 2,6-diisopropylphenyl), [DIPrim] + , and tetrachloroferrate(III) anion, [FeCl 4 ]. This compound was an effective and easy-to-use catalyst for the cross-coupling of aryl Grignard reagents with primary and secondary alkyl halides bearing -hydrogens. After simply decanting the cross-coupling product in the ether layer, [DIPrim][FeCl 4 ] could be reused in at least four successive runs without significant loss of catalytic activity.展开更多
3-(Diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one (DEPBT) was an organophosphorus coupling reagent developed by our group. It was an effective coupling reagent for the synthesis of protected peptides containing T...3-(Diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one (DEPBT) was an organophosphorus coupling reagent developed by our group. It was an effective coupling reagent for the synthesis of protected peptides containing Tyr, Ser and Thr with unprotected hydroxy group on their side chain. The further study of the synthesis of a series of protected dipeptides containing histi-dine with unprotected imidazole group using DEPBT is reported. During the synthetic procedure, the imidazole group of his-tidine did not need to be protected. When the carboxyl components were N-protected aromatic amino acids or basic amino acids, the yields were relatively high (63%-81%). However, when the carboxyl components were N -protected acidic amino acids, the yields were relatively low (47%-48%). The results expanded the application of DEPBT on the synthesis of bioactive peptides containing histidine.展开更多
The efficiency of different peptide coupling reagents, including carbodiimides, HOBt or HOAt-derived uronium, phosphonium and immonium salts, halouronium, halophosphonium, 2-halopyridinium and 2-halothiazolium salts, ...The efficiency of different peptide coupling reagents, including carbodiimides, HOBt or HOAt-derived uronium, phosphonium and immonium salts, halouronium, halophosphonium, 2-halopyridinium and 2-halothiazolium salts, was evaluated. The synthetic strategy for coded peptides and nonribosomal peptides was discussed with an emphasis on the rational selection of peptide coupling reagents.展开更多
文摘Unique coupling reagent, bis-(2-hydroxyethyl methacrylate) phosphate was used to prepare coated and functionalized superparamagnetic nanobeads, leading to a simple, effective method for coating the nanobeads. With this method, the thickness of the coating layer and the functional group contents on the nano-beads could be controlled by changing the quantity of the coated monomers. The nanobeads were characterized by means of transmission electron microscopy (TEM) and Fourier transformation infrared spectroscopy (FTIR). The carboxyl-modified magnetic nano-beads were employed to streamline the protocol of isolation of genomic DNA from the human whole blood.
基金financially supported by the Fundamental Research Funds for the Central Universities,Southwest University for Nationalities (No.12NZYTH03)the Natural Science Foundation of Southwest University for Nationalities (No.381010)+1 种基金the Project of Postgraduate Degree Construction,Southwest University for Nationalities (No.2013XWD-S0703)the State Administration of Foreign Experts Affairs project (No.2012-10)
文摘We describe a convenient method for the synthesis of 1,2-disubstituted acetylenes via a cross-coupling reaction of （bromoethynyl）benzene with Grignard reagents. The reaction of （bromoethynyl）benzene （1 mmol） with Grignard reagent （1.3 mmol） mediated by NiCl2 （4 mol%） and （p-CH3Ph）3P （8 mol%） in THF could produce 1,2-disubstituted acetylenes in good yields at room temperature.
基金supported by the National Natural Science Foundation of China (20772089)the Key Laboratory of Organic Chemistry of Jiangsu Provincethe Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘1,3-Bis(2,6-diisopropylphenyl)imidazolium chloride, [DIPrim]Cl, was used to produce a novel iron(III)-containing imidazolium salt [DIPrim][FeCl 4 ], which included a N,N-diarylimidazolium cation (R = 2,6-diisopropylphenyl), [DIPrim] + , and tetrachloroferrate(III) anion, [FeCl 4 ]. This compound was an effective and easy-to-use catalyst for the cross-coupling of aryl Grignard reagents with primary and secondary alkyl halides bearing -hydrogens. After simply decanting the cross-coupling product in the ether layer, [DIPrim][FeCl 4 ] could be reused in at least four successive runs without significant loss of catalytic activity.
基金Project supported by the National Natural Science Foundation of China(No.20242006).
文摘3-(Diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one (DEPBT) was an organophosphorus coupling reagent developed by our group. It was an effective coupling reagent for the synthesis of protected peptides containing Tyr, Ser and Thr with unprotected hydroxy group on their side chain. The further study of the synthesis of a series of protected dipeptides containing histi-dine with unprotected imidazole group using DEPBT is reported. During the synthetic procedure, the imidazole group of his-tidine did not need to be protected. When the carboxyl components were N-protected aromatic amino acids or basic amino acids, the yields were relatively high (63%-81%). However, when the carboxyl components were N -protected acidic amino acids, the yields were relatively low (47%-48%). The results expanded the application of DEPBT on the synthesis of bioactive peptides containing histidine.
基金Project supported by the National Natural Science Foundation of China.
文摘The efficiency of different peptide coupling reagents, including carbodiimides, HOBt or HOAt-derived uronium, phosphonium and immonium salts, halouronium, halophosphonium, 2-halopyridinium and 2-halothiazolium salts, was evaluated. The synthetic strategy for coded peptides and nonribosomal peptides was discussed with an emphasis on the rational selection of peptide coupling reagents.
