Background： In vivo quantification of choroidal neovascularization （CNV） based on noninvasive optical coherence tomography （OCT） examination and in vitro choroidal flatmount immunohistochemistry stained of CNV cu...Background： In vivo quantification of choroidal neovascularization （CNV） based on noninvasive optical coherence tomography （OCT） examination and in vitro choroidal flatmount immunohistochemistry stained of CNV currently were used to evaluate the process and severity of age-related macular degeneration （AMD） both in human and animal studies. This study aimed to investigate the correlation between these two methods in murine CNV models induced by subretinal injection. Methods： CNV was developed in 20 C57BL6/j mice by subretinal injection of adeno-associated viral delivery of a short hairpin RNA targeting sFLT-1 （AAV.shRNA.sFLT- 1）, as reported previously. After 4 weeks, CNV was imaged by OCT and fluorescence angiography. The scaling factors for each dimension, x, y, and z （ktm/pixel） were recorded, and the corneal curvature standard was adjusted from human （7.7） to mice （1 .4）. The volume of each OCT image stack was calculated and then normalized by multiplying the number of voxels by the scaling factors for each dimension in Seg3D software （University of Utah Scientific Computing and Imaging Institute, available at http：//www.sci.utah.edu/cibc-software/seg3d.html）. Eighteen mice were prepared for choroidal flatmounts and stained by CD31. The CNV volumes were calculated using scanning laser confocal microscopy after immunohistochemistry staining. Two mice were stained by Hematoxylin and Eosin for observing the CNV morphology. Results： The CNV volume calculated using OCT was, on average, 2.6 times larger than the volume calculated using the laser confocal microscopy. The correlation statistical analysis showed OCT measuring of CNV correlated significantly with the in vitro method （R： = 0.448, P = 0.001, n = 18）. The correlation coefficient for CNV quantification using OCT and confocal microscopy was 0.693 （n = 18, P = 0.001 ）. Conclusions： There is a fair linear correlation on CNV volumes between in vivo and in vitro methods in CNV models induced by subretinal inje展开更多
The correlation analysis of sea clutter data in a complex domain is conducted in this study. Specific to X-band sea clutter, the statistical characteristics of the complex correlation, particularly the phase character...The correlation analysis of sea clutter data in a complex domain is conducted in this study. Specific to X-band sea clutter, the statistical characteristics of the complex correlation, particularly the phase characteristics which are closely related to the phase difference of the sea clutter and the Doppler properties, are analyzed in detail based on the experimental data, recorded by the Mc Master University IPIX radar in 1993. That the phase term of the complex correlation presents linear change means that there exists the linearity of phase differences between different time intervals in the X-band sea clutter. This investigation explores the regularities about the effect of wind on the complex correlation with similar patterns for different polarization modes. The regularities indicate that the wind direction can be inferred from the distribution pattern of the complex correlation. Moreover, a model describing the relationships between the statistics of the complex correlation and wind parameters is proposed. The application for target detection based on the differences of characteristics of complex correlations between the sea clutter and the target are also investigated and the proposed features have been confirmed. The principle of the method is fundamental for broader future applications.展开更多
This paper reviews distributed discrimination of strain and temperature by use of an optical fiber based on fiber optic nerve systems. The preliminary method based on multiple resonance peaks of the Brillouin gain spe...This paper reviews distributed discrimination of strain and temperature by use of an optical fiber based on fiber optic nerve systems. The preliminary method based on multiple resonance peaks of the Brillouin gain spectrum in a specially-designed fiber is firstly introduced. The complete discrimination of strain and temperature based on the Brillouin dynamic grating in a polarization maintaining fiber is extensively presented. The basic principle and two experimental schemes of distributed discrimination based on fiber optic nerve systems are demonstrated. The performance of the high discriminative accuracy （0.1 ~C-0.3 ~C and 5 kte-12~te） and high spatial resolution （-10 cm） with the effective measurement points of about 50 for a standard system configuration or about 1000 for a modified one will be highly expected in real industry applications.展开更多
The growth and evolution of domain knowledge have always been the focus of Library and Information Science.Exploring the emergence of correlations in the process of knowledge growth with network science thinking can r...The growth and evolution of domain knowledge have always been the focus of Library and Information Science.Exploring the emergence of correlations in the process of knowledge growth with network science thinking can reveal the growth patterns and mechanisms of knowledge correlations.展开更多
文摘Background： In vivo quantification of choroidal neovascularization （CNV） based on noninvasive optical coherence tomography （OCT） examination and in vitro choroidal flatmount immunohistochemistry stained of CNV currently were used to evaluate the process and severity of age-related macular degeneration （AMD） both in human and animal studies. This study aimed to investigate the correlation between these two methods in murine CNV models induced by subretinal injection. Methods： CNV was developed in 20 C57BL6/j mice by subretinal injection of adeno-associated viral delivery of a short hairpin RNA targeting sFLT-1 （AAV.shRNA.sFLT- 1）, as reported previously. After 4 weeks, CNV was imaged by OCT and fluorescence angiography. The scaling factors for each dimension, x, y, and z （ktm/pixel） were recorded, and the corneal curvature standard was adjusted from human （7.7） to mice （1 .4）. The volume of each OCT image stack was calculated and then normalized by multiplying the number of voxels by the scaling factors for each dimension in Seg3D software （University of Utah Scientific Computing and Imaging Institute, available at http：//www.sci.utah.edu/cibc-software/seg3d.html）. Eighteen mice were prepared for choroidal flatmounts and stained by CD31. The CNV volumes were calculated using scanning laser confocal microscopy after immunohistochemistry staining. Two mice were stained by Hematoxylin and Eosin for observing the CNV morphology. Results： The CNV volume calculated using OCT was, on average, 2.6 times larger than the volume calculated using the laser confocal microscopy. The correlation statistical analysis showed OCT measuring of CNV correlated significantly with the in vitro method （R： = 0.448, P = 0.001, n = 18）. The correlation coefficient for CNV quantification using OCT and confocal microscopy was 0.693 （n = 18, P = 0.001 ）. Conclusions： There is a fair linear correlation on CNV volumes between in vivo and in vitro methods in CNV models induced by subretinal inje
基金supported by the National Natural Science Foundation of China (61271406)
文摘The correlation analysis of sea clutter data in a complex domain is conducted in this study. Specific to X-band sea clutter, the statistical characteristics of the complex correlation, particularly the phase characteristics which are closely related to the phase difference of the sea clutter and the Doppler properties, are analyzed in detail based on the experimental data, recorded by the Mc Master University IPIX radar in 1993. That the phase term of the complex correlation presents linear change means that there exists the linearity of phase differences between different time intervals in the X-band sea clutter. This investigation explores the regularities about the effect of wind on the complex correlation with similar patterns for different polarization modes. The regularities indicate that the wind direction can be inferred from the distribution pattern of the complex correlation. Moreover, a model describing the relationships between the statistics of the complex correlation and wind parameters is proposed. The application for target detection based on the differences of characteristics of complex correlations between the sea clutter and the target are also investigated and the proposed features have been confirmed. The principle of the method is fundamental for broader future applications.
文摘This paper reviews distributed discrimination of strain and temperature by use of an optical fiber based on fiber optic nerve systems. The preliminary method based on multiple resonance peaks of the Brillouin gain spectrum in a specially-designed fiber is firstly introduced. The complete discrimination of strain and temperature based on the Brillouin dynamic grating in a polarization maintaining fiber is extensively presented. The basic principle and two experimental schemes of distributed discrimination based on fiber optic nerve systems are demonstrated. The performance of the high discriminative accuracy （0.1 ~C-0.3 ~C and 5 kte-12~te） and high spatial resolution （-10 cm） with the effective measurement points of about 50 for a standard system configuration or about 1000 for a modified one will be highly expected in real industry applications.
文摘The growth and evolution of domain knowledge have always been the focus of Library and Information Science.Exploring the emergence of correlations in the process of knowledge growth with network science thinking can reveal the growth patterns and mechanisms of knowledge correlations.
