Thermosensitive drug delivery systems （DDSs） face major challenges, such as remote and repeatable control of in vivo temperature, although these can increase the therapeutic efficacy of drugs. To address this issue,...Thermosensitive drug delivery systems （DDSs） face major challenges, such as remote and repeatable control of in vivo temperature, although these can increase the therapeutic efficacy of drugs. To address this issue, we coated near- infrared （NIR） photothermal Cu175S nanocrystals with pH/thermos-sensitive polymer by in situ polymerization. The doxorubicine （DOX） loading content was up to 40 wt.%, with less than 8.2 wt.% of DOX being leaked under normal physiological conditions （pH = 7.4, 37 ~C） for almost 48 h in the absence of NIR light. These nanocapsules demonstrate excellent photothermal stability by continuous long- term NIR irradiation. Based on the stable and high photothermal efficiency （55.8%）, pre-loaded drugs were released as desired using 808-nm light as a trigger. Both in vitro and in vivo antitumor therapy results demonstrated that this smart nanoplatform is an effective agent for synergistic hyperthermia-based chemotherapy of cancer, demonstratin~ remote and noninvasive control.展开更多
The antifouling of marine facilities placed below the waterline is a key constraint to the development of the marine economy.Currently,commercial Cu_(2)O-based antifouling coatings still have defects such as agglomera...The antifouling of marine facilities placed below the waterline is a key constraint to the development of the marine economy.Currently,commercial Cu_(2)O-based antifouling coatings still have defects such as agglomeration,oxidation,short duration and high dosage.For this reason,we developed for the first time a triple antifouling system consisting of Cu_(2)O@poly(NIPAM-co-DAm)(Cu_(2)O@PND)dynamic redox micro-gels triggered by copper ions and polydimethylsiloxane(PDMS).The catechol groups of PND nanogels can chelate Cu^(2+)and reduce it in situ to obtain Cu_(2)O@PND microgels,which guarantees the stable release of Cu^(+).Furthermore,Cu_(2)O remains stable and free from oxidation because of the electron transfer between PND and Cu^(2+).Finally,superior antifouling performance was achieved by combining the stable Cu^(+)re-leased from Cu_(2)O,in situ conversion of PND to hydrogel,and its oxidation to produce active substances and H_(2)O_(2),and PDMS coating with low surface energy.展开更多
Enhancing the antibacterial properties of the currently used implant materials is a major concern of healthcare providers to minimize the impact of peri-implant infection and improve implant survival rates.In this stu...Enhancing the antibacterial properties of the currently used implant materials is a major concern of healthcare providers to minimize the impact of peri-implant infection and improve implant survival rates.In this study,titanium-copper sintered alloys(3 wt% and 5 wt%Cu) were developed for medical implant applications.The phase identification,microstructure,copper ions release,and wettability of these alloys were evaluated using X-ray diffraction(XRD),optical microscope(OM),scanning electron microscope(SEM),transmission electron microscope(TEM),energy-dispersive X-ray spectroscope(EDS),inductively coupled plasma spectroscope(ICP) and drop shape analyzer.The antibacterial activities of Ti-Cu alloys against Porphyromonas gingivalis and Streptococcus mutans were evaluated using inhibition zone assay,plate counting assay,live/dead fluorescent staining,SEM and reactive oxygen species(ROS) staining assay.The results demonstrated that Ti-Cu alloys are composed of α-Ti and Ti_(2)Cu phases that increased with increased Cu content of the alloy.Ti-Cu alloys exhibited antibacterial activity against both bacteria species in a time-dependent manner.The antibacterial rates of Ti-3Cu and Ti-5Cu after72 h were 57% and 70% against Porphyromonas gingivalis and 63% and 78% against Streptococcus mutans,respectively.The highest amount of Cu^(2+) released from these alloys was 0.3 mg·L^(-1) during the first 24 h,within the safe range.The copper ions release and ROS production were suggested as the main antibacterial mechanisms.Overall,Ti-Cu sintered alloys are promising materials for dental and orthopedic implants.展开更多
Severe lesions in vessels need to be treated with implantable interventional devices such as vascular stents,which should be anti‐coagulantion,anti‐proliferation and promoting endothelialisation.Nitric oxide(NO),as ...Severe lesions in vessels need to be treated with implantable interventional devices such as vascular stents,which should be anti‐coagulantion,anti‐proliferation and promoting endothelialisation.Nitric oxide(NO),as a physiological gas signalling molecule,play an important role in revascularisation.Catalysing the release of NO from endogenous do-nors has already been widely favoured to treatment strategy for lesioned vessels.In this work,a series of copper‐loaded coatings(titanium nanotube(TNT)/PDA‐Cu)was fabricated by TNTs combined with polydopamine and ions,which achieve controlled in situ catalytic release of NO.This strategy could effectively immobilised copper ions on TNTs,and promoted the proliferation of endothelial cells and inhibited growth of smooth muscle cells(SMCs)via the performance of NO,as well as restrain the platelet adhesion.With the multiple function,TNT/PDA‐Cu provides a promise approach for promoting endothelialisation,anti‐coagulation and inhibition of SMC proliferation via copper‐loaded coatings on TNTs.展开更多
The design and manufacture of anti-scaling surface is a prospective way to prevent scaling in oil field.In this work,a novel superhydrophobic Cu^(2+)-loaded and DTPMPA-modified anodized copper oxide(S-Cu^(2+)/D-ACO)co...The design and manufacture of anti-scaling surface is a prospective way to prevent scaling in oil field.In this work,a novel superhydrophobic Cu^(2+)-loaded and DTPMPA-modified anodized copper oxide(S-Cu^(2+)/D-ACO)coating was fabricated by modification of 1H,1H,2H,2H-perfluorodecyltriethoxysilane.The valid storing of scale inhibitors at the coating surface and the interfacial release of Cu^(2+)ions contribute to enhancing the anti-scaling of the S-Cu^(2+)/D-ACO coating.The water contact angle of the S-Cu^(2+)/D-ACO coating is 163.03°and exhibits superhydrophobicity,which makes it difficult for CaCO_(3)to deposit at the surface of the coating.DTPMPA will steadily lurk into the inner space,and Cu^(2+)will be loaded at the interface in the form of the DTPMPA:Cu^(2+)chelate.During the deposition of CaCO_(3),the dynamic release of DTPMPA can be realized by transferring DTPMPA:Cu^(2+)to DTPMPA:Ca^(2+).Interestingly,the released Cu^(2+)hinders the active growth of CaCO_(3).After 48 h of scaling,the mass of CaCO_(3)scale at the S-Cu^(2+)/D-ACO coating surface is only 44.1%that of the anodized copper oxide coating.The excellent anti-scaling performance of the S-Cu^(2+)/D-ACO coating is determined by the synergistic effect of the DTPMPA lurking and dynamic release,as well as the Cu^(2+)inhibition at the interface of superhydrophobic coating and against CaCO_(3)deposition.This research provides a new exploration for designing and fabricating anti-scaling superhydrophobic surface for oil field development.展开更多
The concentrations and chemical forms of copper(Cu) and zinc(Zn) in surface soils directly influence the movement of Cu and Zn. In this study, thirteen sandy soil samples with a wide range of total Cu and Zn concentra...The concentrations and chemical forms of copper(Cu) and zinc(Zn) in surface soils directly influence the movement of Cu and Zn. In this study, thirteen sandy soil samples with a wide range of total Cu and Zn concentrations were collected for evaluating the relationships between Cu and Zn release and extraction time, ratio of soil to water, pH and electrolyte types. The results indicated that Cu released in batch extraction that represents long-term leaching was mainly from exchangeable, and carbonate bound Cu fractions, and Zn released in the batch extraction was mainly from its carbonate bound fraction. However, the Cu and Zn leached from the soils using the column leaching that represents short-term leaching were mainly from their exchangeable fractions. Soil column leaching at different pH values indicated that the amounts of leached Zn and Cu were greatly affected by pH. The Cu and Zn release experiments with varying extraction times and ratio of soil to water suggest that long-term water-logging in the soils after rain may increase contact time of the soils with water and the release of Cu and Zn to water from the soils, and total amounts of Cu or Zn released from the soils increase, but the Cu or Zn concentration in the surface runoff decrease with increasing rainfall intensity. The increased Ca concentration in soil solution increased stability of organic matter-mineral complexes and might decrease the dissolution of organic matter, and thus decreased the release of Cu-binding component of organic matter. However, high concentration of Na in the soil solution increased the dispersion of the organic matter-mineral complexes and increased dissolution of organic matter and the release of Cu from the soils.展开更多
The incorporation of therapeutic-capable ions into bioactive glasses(BGs),either based on silica(SBGs)or phosphate(PBGs),is currently envisaged as a proficient path for facilitating bone regeneration.Inconjunction wit...The incorporation of therapeutic-capable ions into bioactive glasses(BGs),either based on silica(SBGs)or phosphate(PBGs),is currently envisaged as a proficient path for facilitating bone regeneration.Inconjunction with this view,the single and complementary structural and bio-functional roles of CuO andGa_(2)O_(3)(in the 2e5 mol%range)were assessed,by deriving a series of SBG and PBG formulations startingfrom the parent glass systems,FastOs®BG e 38.5SiO2d36.1CaOd5.6P2O5d19.2MgOd0.6CaF2,and50.0P2O5d35.0CaOd10.0Na_(2)Od5.0 Fe2O_(3)(mol%),respectively,using the process of melt-quenching.The inter-linked physico-chemistry e biological response of BGs was assessed in search of bio-functional triggers.Further light was shed on the structural role e as network former or modifier e ofCu and Ga,immersed in SBG and PBG matrices.The preliminary biological performance was surveyedin vitro by quantification of Cu and Ga ion release under homeostatic conditions,cytocompatibility assays(in fibroblast cell cultures)and antibacterial tests(against Staphylococcus aureus).The similar(Cu)anddissimilar(Ga)structural roles in the SBG and PBG vitreous networks governed their release.Namely,Cuions were leached in similar concentrations(ranging from 10e35 ppm and 50e110 ppm at BG doses of 5and 50 mg/mL,respectively)for both type of BGs,while the release of Ga ions was 1e2 orders ofmagnitude lower in the case of SBGs(i.e.,0.2e6 ppm)compared to PBGs(i.e.,9e135 ppm).This wasattributed to the network modifier role of Cu in both types of BGs,and conversely,to the network former(SBGs)and network modifier(PBGs)roles of Ga.All glasses were cytocompatible at a dose of 5 mg/mL,while at the same concentration the antimicrobial efficiency was found to be accentuated by the coupledrelease of Cu and Ga ions from SBG.By collective assessment,the most prominent candidate material forthe further development of implant coatings and bone graft substitutes was delineated as the38.5SiO2d34.1CaOd5.6P2O5d16.2MgOd0.6CaF2d2.0CuOd3.0Ga_(2)O_(3)(mol%)SBG system,w展开更多
文摘Thermosensitive drug delivery systems （DDSs） face major challenges, such as remote and repeatable control of in vivo temperature, although these can increase the therapeutic efficacy of drugs. To address this issue, we coated near- infrared （NIR） photothermal Cu175S nanocrystals with pH/thermos-sensitive polymer by in situ polymerization. The doxorubicine （DOX） loading content was up to 40 wt.%, with less than 8.2 wt.% of DOX being leaked under normal physiological conditions （pH = 7.4, 37 ~C） for almost 48 h in the absence of NIR light. These nanocapsules demonstrate excellent photothermal stability by continuous long- term NIR irradiation. Based on the stable and high photothermal efficiency （55.8%）, pre-loaded drugs were released as desired using 808-nm light as a trigger. Both in vitro and in vivo antitumor therapy results demonstrated that this smart nanoplatform is an effective agent for synergistic hyperthermia-based chemotherapy of cancer, demonstratin~ remote and noninvasive control.
基金financially supported by the National Natural Science Foundation of China (No.52073311)the Guangdong Basic and Applied Basic Research Foundation (Nos.2020A1515011274 and 2021A1515012281).
文摘The antifouling of marine facilities placed below the waterline is a key constraint to the development of the marine economy.Currently,commercial Cu_(2)O-based antifouling coatings still have defects such as agglomeration,oxidation,short duration and high dosage.For this reason,we developed for the first time a triple antifouling system consisting of Cu_(2)O@poly(NIPAM-co-DAm)(Cu_(2)O@PND)dynamic redox micro-gels triggered by copper ions and polydimethylsiloxane(PDMS).The catechol groups of PND nanogels can chelate Cu^(2+)and reduce it in situ to obtain Cu_(2)O@PND microgels,which guarantees the stable release of Cu^(+).Furthermore,Cu_(2)O remains stable and free from oxidation because of the electron transfer between PND and Cu^(2+).Finally,superior antifouling performance was achieved by combining the stable Cu^(+)re-leased from Cu_(2)O,in situ conversion of PND to hydrogel,and its oxidation to produce active substances and H_(2)O_(2),and PDMS coating with low surface energy.
基金financially supported by the National Natural Science Foundation of China (Nos.51404302 and 51801003)the Natural Science Foundation of Hunan Province (Nos.2020JJ4732 and 2020JJ4933)。
文摘Enhancing the antibacterial properties of the currently used implant materials is a major concern of healthcare providers to minimize the impact of peri-implant infection and improve implant survival rates.In this study,titanium-copper sintered alloys(3 wt% and 5 wt%Cu) were developed for medical implant applications.The phase identification,microstructure,copper ions release,and wettability of these alloys were evaluated using X-ray diffraction(XRD),optical microscope(OM),scanning electron microscope(SEM),transmission electron microscope(TEM),energy-dispersive X-ray spectroscope(EDS),inductively coupled plasma spectroscope(ICP) and drop shape analyzer.The antibacterial activities of Ti-Cu alloys against Porphyromonas gingivalis and Streptococcus mutans were evaluated using inhibition zone assay,plate counting assay,live/dead fluorescent staining,SEM and reactive oxygen species(ROS) staining assay.The results demonstrated that Ti-Cu alloys are composed of α-Ti and Ti_(2)Cu phases that increased with increased Cu content of the alloy.Ti-Cu alloys exhibited antibacterial activity against both bacteria species in a time-dependent manner.The antibacterial rates of Ti-3Cu and Ti-5Cu after72 h were 57% and 70% against Porphyromonas gingivalis and 63% and 78% against Streptococcus mutans,respectively.The highest amount of Cu^(2+) released from these alloys was 0.3 mg·L^(-1) during the first 24 h,within the safe range.The copper ions release and ROS production were suggested as the main antibacterial mechanisms.Overall,Ti-Cu sintered alloys are promising materials for dental and orthopedic implants.
基金Department of Science and Technology of Sichuan Province,Grant/Award Numbers:2020YFH0103,2022NSFSC0809National Natural Science Foundation of China,Grant/Award Number:NSFC Project 32071328。
文摘Severe lesions in vessels need to be treated with implantable interventional devices such as vascular stents,which should be anti‐coagulantion,anti‐proliferation and promoting endothelialisation.Nitric oxide(NO),as a physiological gas signalling molecule,play an important role in revascularisation.Catalysing the release of NO from endogenous do-nors has already been widely favoured to treatment strategy for lesioned vessels.In this work,a series of copper‐loaded coatings(titanium nanotube(TNT)/PDA‐Cu)was fabricated by TNTs combined with polydopamine and ions,which achieve controlled in situ catalytic release of NO.This strategy could effectively immobilised copper ions on TNTs,and promoted the proliferation of endothelial cells and inhibited growth of smooth muscle cells(SMCs)via the performance of NO,as well as restrain the platelet adhesion.With the multiple function,TNT/PDA‐Cu provides a promise approach for promoting endothelialisation,anti‐coagulation and inhibition of SMC proliferation via copper‐loaded coatings on TNTs.
基金financially supported by the National Science Foundation for Distinguished Young Scholars of China(Grant No.51925403)the Major Research Plan of National Natural Science Foundation of China(Grant No.91934302)the National Science Foundation of China(21676052,21606042)
文摘The design and manufacture of anti-scaling surface is a prospective way to prevent scaling in oil field.In this work,a novel superhydrophobic Cu^(2+)-loaded and DTPMPA-modified anodized copper oxide(S-Cu^(2+)/D-ACO)coating was fabricated by modification of 1H,1H,2H,2H-perfluorodecyltriethoxysilane.The valid storing of scale inhibitors at the coating surface and the interfacial release of Cu^(2+)ions contribute to enhancing the anti-scaling of the S-Cu^(2+)/D-ACO coating.The water contact angle of the S-Cu^(2+)/D-ACO coating is 163.03°and exhibits superhydrophobicity,which makes it difficult for CaCO_(3)to deposit at the surface of the coating.DTPMPA will steadily lurk into the inner space,and Cu^(2+)will be loaded at the interface in the form of the DTPMPA:Cu^(2+)chelate.During the deposition of CaCO_(3),the dynamic release of DTPMPA can be realized by transferring DTPMPA:Cu^(2+)to DTPMPA:Ca^(2+).Interestingly,the released Cu^(2+)hinders the active growth of CaCO_(3).After 48 h of scaling,the mass of CaCO_(3)scale at the S-Cu^(2+)/D-ACO coating surface is only 44.1%that of the anodized copper oxide coating.The excellent anti-scaling performance of the S-Cu^(2+)/D-ACO coating is determined by the synergistic effect of the DTPMPA lurking and dynamic release,as well as the Cu^(2+)inhibition at the interface of superhydrophobic coating and against CaCO_(3)deposition.This research provides a new exploration for designing and fabricating anti-scaling superhydrophobic surface for oil field development.
文摘The concentrations and chemical forms of copper(Cu) and zinc(Zn) in surface soils directly influence the movement of Cu and Zn. In this study, thirteen sandy soil samples with a wide range of total Cu and Zn concentrations were collected for evaluating the relationships between Cu and Zn release and extraction time, ratio of soil to water, pH and electrolyte types. The results indicated that Cu released in batch extraction that represents long-term leaching was mainly from exchangeable, and carbonate bound Cu fractions, and Zn released in the batch extraction was mainly from its carbonate bound fraction. However, the Cu and Zn leached from the soils using the column leaching that represents short-term leaching were mainly from their exchangeable fractions. Soil column leaching at different pH values indicated that the amounts of leached Zn and Cu were greatly affected by pH. The Cu and Zn release experiments with varying extraction times and ratio of soil to water suggest that long-term water-logging in the soils after rain may increase contact time of the soils with water and the release of Cu and Zn to water from the soils, and total amounts of Cu or Zn released from the soils increase, but the Cu or Zn concentration in the surface runoff decrease with increasing rainfall intensity. The increased Ca concentration in soil solution increased stability of organic matter-mineral complexes and might decrease the dissolution of organic matter, and thus decreased the release of Cu-binding component of organic matter. However, high concentration of Na in the soil solution increased the dispersion of the organic matter-mineral complexes and increased dissolution of organic matter and the release of Cu from the soils.
文摘The incorporation of therapeutic-capable ions into bioactive glasses(BGs),either based on silica(SBGs)or phosphate(PBGs),is currently envisaged as a proficient path for facilitating bone regeneration.Inconjunction with this view,the single and complementary structural and bio-functional roles of CuO andGa_(2)O_(3)(in the 2e5 mol%range)were assessed,by deriving a series of SBG and PBG formulations startingfrom the parent glass systems,FastOs®BG e 38.5SiO2d36.1CaOd5.6P2O5d19.2MgOd0.6CaF2,and50.0P2O5d35.0CaOd10.0Na_(2)Od5.0 Fe2O_(3)(mol%),respectively,using the process of melt-quenching.The inter-linked physico-chemistry e biological response of BGs was assessed in search of bio-functional triggers.Further light was shed on the structural role e as network former or modifier e ofCu and Ga,immersed in SBG and PBG matrices.The preliminary biological performance was surveyedin vitro by quantification of Cu and Ga ion release under homeostatic conditions,cytocompatibility assays(in fibroblast cell cultures)and antibacterial tests(against Staphylococcus aureus).The similar(Cu)anddissimilar(Ga)structural roles in the SBG and PBG vitreous networks governed their release.Namely,Cuions were leached in similar concentrations(ranging from 10e35 ppm and 50e110 ppm at BG doses of 5and 50 mg/mL,respectively)for both type of BGs,while the release of Ga ions was 1e2 orders ofmagnitude lower in the case of SBGs(i.e.,0.2e6 ppm)compared to PBGs(i.e.,9e135 ppm).This wasattributed to the network modifier role of Cu in both types of BGs,and conversely,to the network former(SBGs)and network modifier(PBGs)roles of Ga.All glasses were cytocompatible at a dose of 5 mg/mL,while at the same concentration the antimicrobial efficiency was found to be accentuated by the coupledrelease of Cu and Ga ions from SBG.By collective assessment,the most prominent candidate material forthe further development of implant coatings and bone graft substitutes was delineated as the38.5SiO2d34.1CaOd5.6P2O5d16.2MgOd0.6CaF2d2.0CuOd3.0Ga_(2)O_(3)(mol%)SBG system,w
