AIM: Cyclooxygenase-2 (COX-2) has been suggested to be associated with carcinogenesis. We sought to investigate the effect of the selective COX-2 inhibitor, Nimesulide on proliferation and apoptosis of SMMC-7721 human...AIM: Cyclooxygenase-2 (COX-2) has been suggested to be associated with carcinogenesis. We sought to investigate the effect of the selective COX-2 inhibitor, Nimesulide on proliferation and apoptosis of SMMC-7721 human hepatoma cells.METHODS: This study was carried out on the culture of hepatic carcinoma SMMC-7721 cell line. Various concentrations of Nimesulide (0, 200 micromol/L, 300 micromol/L, 400 micromol/L) were added and incubated. Cell proliferation was detected with MTT colorimetric assay, cell apoptosis by electron microscopy, flow cytometry and TUNEL.RESULTS: Nimesulide could significantly inhibit SMMC-7721 cells proliferation dose-dependent and in a dependent manner compared with that of the control group. The duration lowest inhibition rate produced by Nimesulide in SMMC-7721 cells was 19.06%, the highest inhibition rate was 58.49%. After incubation with Nimesulide for 72 h, the most highest apoptosis rate and apoptosis index of SMMC-7721 cells comparing with those of the control were 21.20%+/-1.62% vs 2.24%+/-0.26% and 21.23+/-1.78 vs 2.01+/-0.23 (P【0.05). CONCLUSION:The selective COX-2 inhibitor, Nimesulide can inhibit the proliferation of SMMC-7721 cells and increase apoptosis rate and apoptosis index of SMMC-7721 cells. The apoptosis rate and the apoptosis index are dose-dependent. Under electron microscope SMMC-7721 cells incubated with 300 micromol and 400 micromol Nimesulide show apoptotic characteristics. With the clarification of the mechanism of selective COX-2 inhibitors, These COX-2 selective inhibitors can become the choice of prevention and treatment of cancers.展开更多
为了解决交直流混合微电网中储能单元荷电状态(state of charge,SOC)变化导致的子网互济能力变化和系统供电可靠性问题,提出一种综合考虑子网运行状态和储能SOC变化的交直流混合微电网多模式功率协调控制策略,将系统的运行模式划分为盈...为了解决交直流混合微电网中储能单元荷电状态(state of charge,SOC)变化导致的子网互济能力变化和系统供电可靠性问题,提出一种综合考虑子网运行状态和储能SOC变化的交直流混合微电网多模式功率协调控制策略,将系统的运行模式划分为盈余互济、缺损互济、独立运行和功率共享4种情况,详细研究了在不同运行模式下网间互济功率的传输原则和计算方法,从而实现系统源荷功率平衡和网间SOC均衡,达到了提高交直流混合微电网的可靠性和储能利用效率的目的。最后,在Matlab/Simulink仿真平台上搭建仿真模型并验证了所提多模式控制策略有效性。展开更多
文摘AIM: Cyclooxygenase-2 (COX-2) has been suggested to be associated with carcinogenesis. We sought to investigate the effect of the selective COX-2 inhibitor, Nimesulide on proliferation and apoptosis of SMMC-7721 human hepatoma cells.METHODS: This study was carried out on the culture of hepatic carcinoma SMMC-7721 cell line. Various concentrations of Nimesulide (0, 200 micromol/L, 300 micromol/L, 400 micromol/L) were added and incubated. Cell proliferation was detected with MTT colorimetric assay, cell apoptosis by electron microscopy, flow cytometry and TUNEL.RESULTS: Nimesulide could significantly inhibit SMMC-7721 cells proliferation dose-dependent and in a dependent manner compared with that of the control group. The duration lowest inhibition rate produced by Nimesulide in SMMC-7721 cells was 19.06%, the highest inhibition rate was 58.49%. After incubation with Nimesulide for 72 h, the most highest apoptosis rate and apoptosis index of SMMC-7721 cells comparing with those of the control were 21.20%+/-1.62% vs 2.24%+/-0.26% and 21.23+/-1.78 vs 2.01+/-0.23 (P【0.05). CONCLUSION:The selective COX-2 inhibitor, Nimesulide can inhibit the proliferation of SMMC-7721 cells and increase apoptosis rate and apoptosis index of SMMC-7721 cells. The apoptosis rate and the apoptosis index are dose-dependent. Under electron microscope SMMC-7721 cells incubated with 300 micromol and 400 micromol Nimesulide show apoptotic characteristics. With the clarification of the mechanism of selective COX-2 inhibitors, These COX-2 selective inhibitors can become the choice of prevention and treatment of cancers.
文摘为了解决交直流混合微电网中储能单元荷电状态(state of charge,SOC)变化导致的子网互济能力变化和系统供电可靠性问题,提出一种综合考虑子网运行状态和储能SOC变化的交直流混合微电网多模式功率协调控制策略,将系统的运行模式划分为盈余互济、缺损互济、独立运行和功率共享4种情况,详细研究了在不同运行模式下网间互济功率的传输原则和计算方法,从而实现系统源荷功率平衡和网间SOC均衡,达到了提高交直流混合微电网的可靠性和储能利用效率的目的。最后,在Matlab/Simulink仿真平台上搭建仿真模型并验证了所提多模式控制策略有效性。
