AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell...AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS: The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P【0.05 and P【0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h), B(1) (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D(1) (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B(1) and D(1) were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P【0.01), whereas the expressions of P16(ink4a) and P21(cip/waf1), cyclin-dependent kinases inhibitors(CDKI), were increased. CONCLUSION: The cell growth and proliferation of SGC-7901 cell is inhibited by c9, t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B(1) and D(1) and enhanced expressions of CDKI(P16(ink4a) and p21(cip/waf1)).展开更多
Two extended hybrid conjugated systems based on a triphenylamine(TPA) core with two and three peripheral 1,4-dithiafulvenes(DTF) units coded WH-2 and WH-3 as hole-transporting materials(HTMs) applied in perovskite sol...Two extended hybrid conjugated systems based on a triphenylamine(TPA) core with two and three peripheral 1,4-dithiafulvenes(DTF) units coded WH-2 and WH-3 as hole-transporting materials(HTMs) applied in perovskite solar cells(PSCs) are synthesized by facile one-step reaction in good yield over 75%. DTF unit as electron donor can enhance the electron donating ability and the fusion of benzenic ring of TPA with DTF unit may lead to reinforced intermolecular interactions in the solid state. In addition,WH-2 and WH-3 exhibit a pyramid shape containing partial planarity and quasi three-dimensionality features, which is also conducive to enhancing the π-π stacking of molecules in the solid state. The above-mentioned structural characteristics make the two HTMs have good hole mobilities. As a result,WH-2 and WH-3 obtained the high intrinsic hole mobilities of 4.69 × 10^(-4)and 2.18 × 10^(-3)cm^(2)V^(-1)s^(-1)respectively. Finally, the power conversion efficiencies(PCEs) of PSCs with WH-2 and WH-3 as cost-effective dopant-free HTMs are 15.39% and 19.22% respectively and the PCE of PSC with WH-3 is on a par with that of PSC with Li-TFSI/t-BP doped Spiro-OMe TAD(19.67%).展开更多
Five 4,7-dithien-2-yl-2,1,3-benzothiadiazole （DTBT）-based conjugated copolymers with controlled molecular weight were synthesized to explore their optical, energy level and photovoltaic properties. By tuning the pos...Five 4,7-dithien-2-yl-2,1,3-benzothiadiazole （DTBT）-based conjugated copolymers with controlled molecular weight were synthesized to explore their optical, energy level and photovoltaic properties. By tuning the positions of hexyl side chains on DTBT unit, the DTBT- fluorene copolymers exhibited very different aggregation properties, leading to 60 nm bathochromic shift in their absorptions and the corresponding power conversion efficiencies （PCEs） value of photovoltaic cells varied from 0.38%, 0.69% to 2.47%. Different copolymerization units, fluorene, earbazole and phenothiazine were also investigated. The polymer based on phenothiazine exhib- ited lower PCE value due to much lower molecular weight owing to its poor solubility, although phenothiazine units were expected to be a better electron donor. Compared with the fluorene-based polymer, the carbazole-DTBT copolymer showed higher short circuit current density （Jsc） and PCE value due to its better intermolecular stacking,展开更多
We report an improved strategy for conjugated polymer-based optical DNA detection with stem-loop structured DNA probes.The high sensitivity of conjugated polymer-based biosensors arises from light harvesting by the co...We report an improved strategy for conjugated polymer-based optical DNA detection with stem-loop structured DNA probes.The high sensitivity of conjugated polymer-based biosensors arises from light harvesting by the conjugated polymer and the related amplified fluorescent signal transduction either through quenching or through Fǒrster energy transfer) In our previous publication,we demonstrated that the coupling of magnetic particles to conjugated polymers significantly improves the selectivity of this class of DNA sensors.Here we further improved the selectivity by introducing both stem-loop structured capture probe and competition probe.We demonstrated further that this improved magnetically assisted DNA sensor can conveniently identify even a single-nucleotide mismatch in the target sequence.展开更多
A novel organocatalytic cascade process initiated by 1,6-conjugated addition has been successfully developed. A range of pharmaceutically active 2-amino-4-aryl-4H-chromenes were readily obtained in high yields（88%-99...A novel organocatalytic cascade process initiated by 1,6-conjugated addition has been successfully developed. A range of pharmaceutically active 2-amino-4-aryl-4H-chromenes were readily obtained in high yields（88%-99%） and excellent enantiopurities（86%-99% ee）. The functionalized para-Quinone methides（p-OMs） could be facilely obtained.展开更多
Theoretical calculations via the transfer matrix method （TMM） are performed to investigate optical properties of one-dimensional semiconductor-organic photonic crystals （SOPC） with periodic conjugated polymer （3-...Theoretical calculations via the transfer matrix method （TMM） are performed to investigate optical properties of one-dimensional semiconductor-organic photonic crystals （SOPC） with periodic conjugated polymer （3-octylthiophenes,P3OT）/AIN multilayer structure. The SOPC presents incomplete photonic band gap behavior in the UV region. P3OT/A1N multilayers with two pairs of 30nm-P3OT and 30nm-A1N layers exhibit a photonic band gap at a central wave- length of about 275nm,and the highest reflectivity reaches 98%. Furthermore,the band gaps are confirmed to be tunable by adjusting the lattice period and the filling fraction. As a consequence, the SOPC is important for achieving materials with an incomplete band gap in the UV region.展开更多
基金the National Natural Science Foundation of China,No.39870661
文摘AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS: The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P【0.05 and P【0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h), B(1) (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D(1) (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B(1) and D(1) were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P【0.01), whereas the expressions of P16(ink4a) and P21(cip/waf1), cyclin-dependent kinases inhibitors(CDKI), were increased. CONCLUSION: The cell growth and proliferation of SGC-7901 cell is inhibited by c9, t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B(1) and D(1) and enhanced expressions of CDKI(P16(ink4a) and p21(cip/waf1)).
基金the Sichuan Science and Technology Program (2019YJ0162)the National Natural Science Foundation of China (21402023, 51773027)the National Key R@D Program of China (2017YFB0702802) for financial support。
文摘Two extended hybrid conjugated systems based on a triphenylamine(TPA) core with two and three peripheral 1,4-dithiafulvenes(DTF) units coded WH-2 and WH-3 as hole-transporting materials(HTMs) applied in perovskite solar cells(PSCs) are synthesized by facile one-step reaction in good yield over 75%. DTF unit as electron donor can enhance the electron donating ability and the fusion of benzenic ring of TPA with DTF unit may lead to reinforced intermolecular interactions in the solid state. In addition,WH-2 and WH-3 exhibit a pyramid shape containing partial planarity and quasi three-dimensionality features, which is also conducive to enhancing the π-π stacking of molecules in the solid state. The above-mentioned structural characteristics make the two HTMs have good hole mobilities. As a result,WH-2 and WH-3 obtained the high intrinsic hole mobilities of 4.69 × 10^(-4)and 2.18 × 10^(-3)cm^(2)V^(-1)s^(-1)respectively. Finally, the power conversion efficiencies(PCEs) of PSCs with WH-2 and WH-3 as cost-effective dopant-free HTMs are 15.39% and 19.22% respectively and the PCE of PSC with WH-3 is on a par with that of PSC with Li-TFSI/t-BP doped Spiro-OMe TAD(19.67%).
基金Acknowledgements Financial support by the National Natural Science Foundation of China （Grant Nos. 51073063 and 20904057） and Open Project of State Key Laboralory for Supramolecular Structure and Materials （No. SKLSSM201129） of Jilin university arc gratefully acknowledged. J. Zhang thanks the support by 100 Talents Programme of the Chinese Academy of Science.
文摘Five 4,7-dithien-2-yl-2,1,3-benzothiadiazole （DTBT）-based conjugated copolymers with controlled molecular weight were synthesized to explore their optical, energy level and photovoltaic properties. By tuning the positions of hexyl side chains on DTBT unit, the DTBT- fluorene copolymers exhibited very different aggregation properties, leading to 60 nm bathochromic shift in their absorptions and the corresponding power conversion efficiencies （PCEs） value of photovoltaic cells varied from 0.38%, 0.69% to 2.47%. Different copolymerization units, fluorene, earbazole and phenothiazine were also investigated. The polymer based on phenothiazine exhib- ited lower PCE value due to much lower molecular weight owing to its poor solubility, although phenothiazine units were expected to be a better electron donor. Compared with the fluorene-based polymer, the carbazole-DTBT copolymer showed higher short circuit current density （Jsc） and PCE value due to its better intermolecular stacking,
基金We greatly appreciate the support from National Natural Science Foundation （20404016, 60537030）；Shanghai Municipal Commission for Science and Technology （0452nm068, 03DZ14025）；the program for Shanghai Rising Star and Chinese Academy of Sciences.
文摘We report an improved strategy for conjugated polymer-based optical DNA detection with stem-loop structured DNA probes.The high sensitivity of conjugated polymer-based biosensors arises from light harvesting by the conjugated polymer and the related amplified fluorescent signal transduction either through quenching or through Fǒrster energy transfer) In our previous publication,we demonstrated that the coupling of magnetic particles to conjugated polymers significantly improves the selectivity of this class of DNA sensors.Here we further improved the selectivity by introducing both stem-loop structured capture probe and competition probe.We demonstrated further that this improved magnetically assisted DNA sensor can conveniently identify even a single-nucleotide mismatch in the target sequence.
基金financially supported by the National Natural Science Foundation of China(No.21402163)the Fundamental Research Funds for the Central Universities of Southwest Minzu University (No. 2016NGJPY02)+1 种基金the Graduate Innovation Project of Southwest Minzu University (No. CX2017SZ016)the Undergraduate Innovation Project of Southwest Minzu University (No. S201610656092)
文摘A novel organocatalytic cascade process initiated by 1,6-conjugated addition has been successfully developed. A range of pharmaceutically active 2-amino-4-aryl-4H-chromenes were readily obtained in high yields（88%-99%） and excellent enantiopurities（86%-99% ee）. The functionalized para-Quinone methides（p-OMs） could be facilely obtained.
文摘Theoretical calculations via the transfer matrix method （TMM） are performed to investigate optical properties of one-dimensional semiconductor-organic photonic crystals （SOPC） with periodic conjugated polymer （3-octylthiophenes,P3OT）/AIN multilayer structure. The SOPC presents incomplete photonic band gap behavior in the UV region. P3OT/A1N multilayers with two pairs of 30nm-P3OT and 30nm-A1N layers exhibit a photonic band gap at a central wave- length of about 275nm,and the highest reflectivity reaches 98%. Furthermore,the band gaps are confirmed to be tunable by adjusting the lattice period and the filling fraction. As a consequence, the SOPC is important for achieving materials with an incomplete band gap in the UV region.
