Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for st...Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for studying human disease mechanisms in vitro by making it possible to circumvent the ethical issues of human embryonic stem cell research.However,significant limitations exist when using conventional flat culturing methods especially concerning cell expansion,differentiation efficiency,stability maintenance and multicellular 3D structure establishment,differentiation prediction.Embryoid bodies(EBs),the multicellular aggregates spontaneously generated from iPSCs in the suspension system,might help to address these issues.Due to the unique microenvironment and cell communication in EB structure that a 2D culture system cannot achieve,EBs have been widely applied in hiPSC-derived differentiation and show significant advantages especially in scaling up culturing,differentiation efficiency enhancement,ex vivo simulation,and organoid establishment.EBs can potentially also be used in early prediction of iPSC differentiation capability.To improve the stability and feasibility of EB-mediated differentiation and generate high quality EBs,critical factors including iPSC pluripotency maintenance,generation of uniform morphology using micro-pattern 3D culture systems,proper cellular density inoculation,and EB size control are discussed on the basis of both published data and our own laboratory experiences.Collectively,the production of a large quantity of homogeneous EBs with high quality is important for the stability and feasibility of many PSCs related studies.展开更多
China's new-energy vehicle(NEV)industry has taken the global lead in breaking through technological barriers.It has secured the world's top position in production and sales for nine consecutive years,with a ma...China's new-energy vehicle(NEV)industry has taken the global lead in breaking through technological barriers.It has secured the world's top position in production and sales for nine consecutive years,with a market share of more than 60 percent.展开更多
Meizhou is committed to creating a more open and inclusive"Capital of Hakka Culture",fully demonstrating its unique cultural charm,so as to provide strong spiritual power and cultural support for Meizhou'...Meizhou is committed to creating a more open and inclusive"Capital of Hakka Culture",fully demonstrating its unique cultural charm,so as to provide strong spiritual power and cultural support for Meizhou's revitalization acceleration and commonprosperity.展开更多
The Uranium-238 (<sup>238</sup>U), Thorium-232 (<sup>232</sup>Th) families and Potassium-40 (<sup>40</sup>K) are of terrestrial origin and contribute generally to an individual’s e...The Uranium-238 (<sup>238</sup>U), Thorium-232 (<sup>232</sup>Th) families and Potassium-40 (<sup>40</sup>K) are of terrestrial origin and contribute generally to an individual’s external exposure through our presence in this environment. They also contribute to the internal exposure through the ingestion of products and beverages such as water that are close to the earth. The aim of this work is to determine the committed effective dose or Total Indicative Dose (TID) due to gamma radioactivity of the borehole water from the Nord Riviera (NR) well field operated by the Côte d’Ivoire Water Distribution Company (SODECI) for the supply of drinking water to part of the population of Abidjan. In addition, the populations, with their habits, could use these borehole waters directly as drinking water. To this end, water samples from the seven (07) functional boreholes were collected and analyzed on a gamma spectrometry chain, equipped with an HPGe detector in the laboratory of the Radiation Protection Institute (RPI) of the GHANA Atomic Energy Commission (GAEC). The results of the specific activities of <sup>238</sup>U, <sup>232</sup>Th and <sup>40</sup>K obtained were transcribed into TID. As the natural radioactivity of the borehole water is high [1], the TIDs calculated from the activity results of the natural radionuclides<sup>238</sup>U, <sup>232</sup>Th, and <sup>40</sup>K vary for the seven boreholes from 0.150 to 0.166 mSv/yr with an average of 0.161 ± 0.034 mSv/yr. The TID of the control tower, where the borehole water is mixed and treated for household use, is equal to 0.136 ± 0.03 mSv/yr. The TIDs obtained are therefore all slightly greater than the WHO reference dose value of 0.1 mSv/yr. But all remain below the UNSCEAR reference dose of 0.29 mSv/yr.展开更多
This special issue is committed to celebrating the 120th Anniversary of Shandong University(SDU)(15th October,2021),as well as capturing the most fascinating research works and reviews from all aspects of optics and p...This special issue is committed to celebrating the 120th Anniversary of Shandong University(SDU)(15th October,2021),as well as capturing the most fascinating research works and reviews from all aspects of optics and photonics,including but not limited to,optoelectronic functional crystals,basic science,applied and engineering research and applications.展开更多
While the world remains committed to upholding the principle of human rights protection,this universal sacrosanct value of humanity has been endlessly put to severe tests in the prevailing global governance.The incohe...While the world remains committed to upholding the principle of human rights protection,this universal sacrosanct value of humanity has been endlessly put to severe tests in the prevailing global governance.The incoherent global response to the Covid-19 pandemic in 2020 was merely the proverbial last straw that broke the camel’s back.展开更多
Liu Xuezhou,a 17-year-old from Hebei Province,committed suicide in Sanya,Hainan Province,in January 2022.Liu,who had been sold by his parents at birth,was reunited with them,respectively,in December 2021 and January 2...Liu Xuezhou,a 17-year-old from Hebei Province,committed suicide in Sanya,Hainan Province,in January 2022.Liu,who had been sold by his parents at birth,was reunited with them,respectively,in December 2021 and January 2022,only to be abandoned by them again soon after.展开更多
A Chinese scientist and his university are committed to improving the health situation on the African continent For around 30 years, Li Xiaokun has been in charge of his research team in China studying growth factors....A Chinese scientist and his university are committed to improving the health situation on the African continent For around 30 years, Li Xiaokun has been in charge of his research team in China studying growth factors. Li, who was born in 1964, spent his early years in Fuping County of Shaanxi Province, where he frequently saw his relatives’lives being ruined by illness. These events caused him pain, but they also triggered his passion for medicine.展开更多
Background: Numerous studies of tissues’ regeneration have confessed the recovery of damaged liver by hematopoietic stem cells. The cells act not only by cell replacement in the target organ but also by delivering tr...Background: Numerous studies of tissues’ regeneration have confessed the recovery of damaged liver by hematopoietic stem cells. The cells act not only by cell replacement in the target organ but also by delivering trophic factors that support endogenous liver regeneration. A little is known of how organ-derived signals recruit such committed cells into circulation. Objective: We investigated the roles of noninvasive mechanical percutaneous stress of cirrhotic human liver in numbers fluctuation of trophic, liver-specific alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells in lymphocytes of patients waiting for liver transplantation. Methods: To promote in blood the number of the alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells, committed to liver’ tissue, we activated mechanically the cirrhotic liver of patient by transcutaneous micro vibration received from skin-contacted electro-magnetic vibraphones generated mechanical pulses with amplitude 10 μm and smoothly changing frequency from 0.03 kHz to 18 kHz and back forth during one cycle duration 1 minute. The number of the alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells in lymphocytes of potential recipients was controlled by flow cytometry before and during daily sonication of skin area, which corresponds to liver projection on it. The 15 minutes cyclic sonication of the liver area performed daily for three weeks. Results: The sonication increased significantly averaged number of liver-specific alpha-fetoprotein-positive CD133-positive blood lymphocytes in 2 - 3 times compared to a base lane. The second similar sonication, the same zone after three weeks break showed differences with baseline, but it was statistically insignificant. The result was specifically related to the liver as it showed the control sonication of the backbone’s projection on the skin of a separate group of patients with cirrhotic liver from the waiting list. Conclusion: The stem cells committed to 展开更多
Dear Editor,Dendritic cells(DCs)are the most efficient professional antigen presenting cells that act as sentinels of the immune system and conduct vital functions in the initiation and regulation of innate and adapti...Dear Editor,Dendritic cells(DCs)are the most efficient professional antigen presenting cells that act as sentinels of the immune system and conduct vital functions in the initiation and regulation of innate and adaptive immunities(Wculek et al.,2020).In addition to being critical cellular components in pathogen clearance,DCs are promising targets for improved tumor immunotherapy,vaccine design,and intervention of autoimmune diseases(Nutt and Chopin,2020).DCs can be classified as plasmacytoid DCs(pDCs)and conventional DCs(cDCs)in both mouse and human(Anderson et al.,2018;Nutt and Chopin,2020).展开更多
We use a nuclear technique based on the determination of the detection efficiencies of solid state nuclear track detectors CR-39 and LR-115 type II (SSNTDs) for alpha particles emitted from the series of uranium-238 a...We use a nuclear technique based on the determination of the detection efficiencies of solid state nuclear track detectors CR-39 and LR-115 type II (SSNTDs) for alpha particles emitted from the series of uranium-238 and thorium-232 in a phytotherapeutic sample and the measurement of alpha track densities registered on these detectors to assess alpha activities due to uranium-238;thorium-232;radon and thoron in samples of phytotherapeutic preparations consumed by Moroccan adult patients. For modern preparations, the alpha activities due to <sup>238</sup>U, <sup>232</sup>Th and <sup>222</sup>Rn range from 14.27 mBq/kg to 22.02 mBq/kg, from 6.27 mBq/kg to 9.64 mBq/kg and from 14.27 Bq/kg to 22.02 Bq/kg respectively. For classical preparations, the alpha activities due to <sup>238</sup>U, <sup>232</sup>Th and <sup>222</sup>Rn range from 16.73 mBq/kg to 24 mBq/kg, from 7.34 mBq/kg to 10.82 mBq/kg and from 16.73 Bq/kg to 24.72 Bq/kg respectively. A dosimetric model for ingestion has been highlighted to determine committed equivalent dose to different compartments of human gastrointestinal system due to the ingestion of phytotherapeutic preparations by Moroccan adult patients. The maximum overall effective dose due to <sup>238</sup>U, <sup>232</sup>Th, and <sup>222</sup>Rn after the ingestion of the studied phytotherapeutic preparations, was found equal to 38 × 10<sup>-8</sup> S·vy<sup>-1</sup> which is less than the dose limit given by the international commission for radiological protection in it publication 56.展开更多
Criminal corruption refers to instances in which employees of the government,private companies or other work units take advantage of their position to embezzle funds or accept bribes.The state of criminal corruption c...Criminal corruption refers to instances in which employees of the government,private companies or other work units take advantage of their position to embezzle funds or accept bribes.The state of criminal corruption can be assessed both objectively and subjectively;both methods exhibit insufficient accuracy in some respects.Presently,criminal corruption is a severe problem in China,~1 having become both systemic and socially ingrained.Causes of corruption can be traced back to human nature and instincts,social practices,and the general operation of the systems in question.A clear understanding of these causes can provide us with a method for developing countermeasures.展开更多
AfriCA Healthcare needs Dose of Money The World Health Organization（WHO）has called on African governments to invest more to improve the quality of healthcare services.WHO Director for Africa Matshidiso Moeti said he...AfriCA Healthcare needs Dose of Money The World Health Organization（WHO）has called on African governments to invest more to improve the quality of healthcare services.WHO Director for Africa Matshidiso Moeti said healthcare funding in Africa is still below the required threshold.展开更多
The 70th anniversary of the victory of the Chinese People’s War of Resistance against Japanese Aggression and the World Anti-Fascist War is an important opportunity for victors to draw strength from the
INTRODUCTION On October 14,1936,Shipping Tycoon Chen Shuntong(STC),1upon request of the Japanese Daido Kaiun Corporation(Daido Kaiun),and on behalf of his Shanghai Chung Wei Steamship Co.(Shanghai CW),drafted a time c...INTRODUCTION On October 14,1936,Shipping Tycoon Chen Shuntong(STC),1upon request of the Japanese Daido Kaiun Corporation(Daido Kaiun),and on behalf of his Shanghai Chung Wei Steamship Co.(Shanghai CW),drafted a time charter which stipulated a charter period of 13calendar months from the delivery date of November 1 of 1936 to December 1 of 1937 to rent out his 6。展开更多
By recognizing and publishing the special contributions and achievements of professionals in environmental science and engineering,Frontiers of Environmental Science&Engineering is committed to become a vehicle fo...By recognizing and publishing the special contributions and achievements of professionals in environmental science and engineering,Frontiers of Environmental Science&Engineering is committed to become a vehicle for innovation,development,and application of solutions for environmental protection and sustainable development,and to serve the environmental research community.展开更多
基金Supported by National Natural Science Foundation of China,No.81770621,No.81573053Ministry of Education,Culture,Sports,Science,and Technology of Japan,KAKENHI,No.16K15604,No.18H02866Natural Science Foundation of Jiangsu Province,No.BK20180281
文摘Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for studying human disease mechanisms in vitro by making it possible to circumvent the ethical issues of human embryonic stem cell research.However,significant limitations exist when using conventional flat culturing methods especially concerning cell expansion,differentiation efficiency,stability maintenance and multicellular 3D structure establishment,differentiation prediction.Embryoid bodies(EBs),the multicellular aggregates spontaneously generated from iPSCs in the suspension system,might help to address these issues.Due to the unique microenvironment and cell communication in EB structure that a 2D culture system cannot achieve,EBs have been widely applied in hiPSC-derived differentiation and show significant advantages especially in scaling up culturing,differentiation efficiency enhancement,ex vivo simulation,and organoid establishment.EBs can potentially also be used in early prediction of iPSC differentiation capability.To improve the stability and feasibility of EB-mediated differentiation and generate high quality EBs,critical factors including iPSC pluripotency maintenance,generation of uniform morphology using micro-pattern 3D culture systems,proper cellular density inoculation,and EB size control are discussed on the basis of both published data and our own laboratory experiences.Collectively,the production of a large quantity of homogeneous EBs with high quality is important for the stability and feasibility of many PSCs related studies.
文摘China's new-energy vehicle(NEV)industry has taken the global lead in breaking through technological barriers.It has secured the world's top position in production and sales for nine consecutive years,with a market share of more than 60 percent.
文摘Meizhou is committed to creating a more open and inclusive"Capital of Hakka Culture",fully demonstrating its unique cultural charm,so as to provide strong spiritual power and cultural support for Meizhou's revitalization acceleration and commonprosperity.
文摘The Uranium-238 (<sup>238</sup>U), Thorium-232 (<sup>232</sup>Th) families and Potassium-40 (<sup>40</sup>K) are of terrestrial origin and contribute generally to an individual’s external exposure through our presence in this environment. They also contribute to the internal exposure through the ingestion of products and beverages such as water that are close to the earth. The aim of this work is to determine the committed effective dose or Total Indicative Dose (TID) due to gamma radioactivity of the borehole water from the Nord Riviera (NR) well field operated by the Côte d’Ivoire Water Distribution Company (SODECI) for the supply of drinking water to part of the population of Abidjan. In addition, the populations, with their habits, could use these borehole waters directly as drinking water. To this end, water samples from the seven (07) functional boreholes were collected and analyzed on a gamma spectrometry chain, equipped with an HPGe detector in the laboratory of the Radiation Protection Institute (RPI) of the GHANA Atomic Energy Commission (GAEC). The results of the specific activities of <sup>238</sup>U, <sup>232</sup>Th and <sup>40</sup>K obtained were transcribed into TID. As the natural radioactivity of the borehole water is high [1], the TIDs calculated from the activity results of the natural radionuclides<sup>238</sup>U, <sup>232</sup>Th, and <sup>40</sup>K vary for the seven boreholes from 0.150 to 0.166 mSv/yr with an average of 0.161 ± 0.034 mSv/yr. The TID of the control tower, where the borehole water is mixed and treated for household use, is equal to 0.136 ± 0.03 mSv/yr. The TIDs obtained are therefore all slightly greater than the WHO reference dose value of 0.1 mSv/yr. But all remain below the UNSCEAR reference dose of 0.29 mSv/yr.
文摘This special issue is committed to celebrating the 120th Anniversary of Shandong University(SDU)(15th October,2021),as well as capturing the most fascinating research works and reviews from all aspects of optics and photonics,including but not limited to,optoelectronic functional crystals,basic science,applied and engineering research and applications.
文摘While the world remains committed to upholding the principle of human rights protection,this universal sacrosanct value of humanity has been endlessly put to severe tests in the prevailing global governance.The incoherent global response to the Covid-19 pandemic in 2020 was merely the proverbial last straw that broke the camel’s back.
文摘Liu Xuezhou,a 17-year-old from Hebei Province,committed suicide in Sanya,Hainan Province,in January 2022.Liu,who had been sold by his parents at birth,was reunited with them,respectively,in December 2021 and January 2022,only to be abandoned by them again soon after.
文摘A Chinese scientist and his university are committed to improving the health situation on the African continent For around 30 years, Li Xiaokun has been in charge of his research team in China studying growth factors. Li, who was born in 1964, spent his early years in Fuping County of Shaanxi Province, where he frequently saw his relatives’lives being ruined by illness. These events caused him pain, but they also triggered his passion for medicine.
文摘Background: Numerous studies of tissues’ regeneration have confessed the recovery of damaged liver by hematopoietic stem cells. The cells act not only by cell replacement in the target organ but also by delivering trophic factors that support endogenous liver regeneration. A little is known of how organ-derived signals recruit such committed cells into circulation. Objective: We investigated the roles of noninvasive mechanical percutaneous stress of cirrhotic human liver in numbers fluctuation of trophic, liver-specific alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells in lymphocytes of patients waiting for liver transplantation. Methods: To promote in blood the number of the alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells, committed to liver’ tissue, we activated mechanically the cirrhotic liver of patient by transcutaneous micro vibration received from skin-contacted electro-magnetic vibraphones generated mechanical pulses with amplitude 10 μm and smoothly changing frequency from 0.03 kHz to 18 kHz and back forth during one cycle duration 1 minute. The number of the alpha-fetoprotein-positive fraction of CD133-positive hematopoietic stem cells in lymphocytes of potential recipients was controlled by flow cytometry before and during daily sonication of skin area, which corresponds to liver projection on it. The 15 minutes cyclic sonication of the liver area performed daily for three weeks. Results: The sonication increased significantly averaged number of liver-specific alpha-fetoprotein-positive CD133-positive blood lymphocytes in 2 - 3 times compared to a base lane. The second similar sonication, the same zone after three weeks break showed differences with baseline, but it was statistically insignificant. The result was specifically related to the liver as it showed the control sonication of the backbone’s projection on the skin of a separate group of patients with cirrhotic liver from the waiting list. Conclusion: The stem cells committed to
文摘Dear Editor,Dendritic cells(DCs)are the most efficient professional antigen presenting cells that act as sentinels of the immune system and conduct vital functions in the initiation and regulation of innate and adaptive immunities(Wculek et al.,2020).In addition to being critical cellular components in pathogen clearance,DCs are promising targets for improved tumor immunotherapy,vaccine design,and intervention of autoimmune diseases(Nutt and Chopin,2020).DCs can be classified as plasmacytoid DCs(pDCs)and conventional DCs(cDCs)in both mouse and human(Anderson et al.,2018;Nutt and Chopin,2020).
文摘We use a nuclear technique based on the determination of the detection efficiencies of solid state nuclear track detectors CR-39 and LR-115 type II (SSNTDs) for alpha particles emitted from the series of uranium-238 and thorium-232 in a phytotherapeutic sample and the measurement of alpha track densities registered on these detectors to assess alpha activities due to uranium-238;thorium-232;radon and thoron in samples of phytotherapeutic preparations consumed by Moroccan adult patients. For modern preparations, the alpha activities due to <sup>238</sup>U, <sup>232</sup>Th and <sup>222</sup>Rn range from 14.27 mBq/kg to 22.02 mBq/kg, from 6.27 mBq/kg to 9.64 mBq/kg and from 14.27 Bq/kg to 22.02 Bq/kg respectively. For classical preparations, the alpha activities due to <sup>238</sup>U, <sup>232</sup>Th and <sup>222</sup>Rn range from 16.73 mBq/kg to 24 mBq/kg, from 7.34 mBq/kg to 10.82 mBq/kg and from 16.73 Bq/kg to 24.72 Bq/kg respectively. A dosimetric model for ingestion has been highlighted to determine committed equivalent dose to different compartments of human gastrointestinal system due to the ingestion of phytotherapeutic preparations by Moroccan adult patients. The maximum overall effective dose due to <sup>238</sup>U, <sup>232</sup>Th, and <sup>222</sup>Rn after the ingestion of the studied phytotherapeutic preparations, was found equal to 38 × 10<sup>-8</sup> S·vy<sup>-1</sup> which is less than the dose limit given by the international commission for radiological protection in it publication 56.
文摘Criminal corruption refers to instances in which employees of the government,private companies or other work units take advantage of their position to embezzle funds or accept bribes.The state of criminal corruption can be assessed both objectively and subjectively;both methods exhibit insufficient accuracy in some respects.Presently,criminal corruption is a severe problem in China,~1 having become both systemic and socially ingrained.Causes of corruption can be traced back to human nature and instincts,social practices,and the general operation of the systems in question.A clear understanding of these causes can provide us with a method for developing countermeasures.
文摘AfriCA Healthcare needs Dose of Money The World Health Organization（WHO）has called on African governments to invest more to improve the quality of healthcare services.WHO Director for Africa Matshidiso Moeti said healthcare funding in Africa is still below the required threshold.
文摘The 70th anniversary of the victory of the Chinese People’s War of Resistance against Japanese Aggression and the World Anti-Fascist War is an important opportunity for victors to draw strength from the
文摘INTRODUCTION On October 14,1936,Shipping Tycoon Chen Shuntong(STC),1upon request of the Japanese Daido Kaiun Corporation(Daido Kaiun),and on behalf of his Shanghai Chung Wei Steamship Co.(Shanghai CW),drafted a time charter which stipulated a charter period of 13calendar months from the delivery date of November 1 of 1936 to December 1 of 1937 to rent out his 6。
文摘By recognizing and publishing the special contributions and achievements of professionals in environmental science and engineering,Frontiers of Environmental Science&Engineering is committed to become a vehicle for innovation,development,and application of solutions for environmental protection and sustainable development,and to serve the environmental research community.
