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菊苣小分子化合物对黄嘌呤氧化酶抑制作用的分子对接研究 被引量:24
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作者 王雪洁 林志健 +5 位作者 张冰 朱春胜 牛红娟 周月 聂安政 王雨 《中国中药杂志》 CAS CSCD 北大核心 2015年第19期3818-3825,共8页
黄嘌呤氧化酶是治疗高尿酸血症药物的关键靶点,中药菊苣提取物具黄嘌呤氧化酶抑制作用,但作用机制仍未探明。该文通过检索相关文献及数据库收集菊苣已知小分子化合物建立配体库,从PDB数据库下载黄嘌呤氧化酶X-ray晶体三维结构文件,采用A... 黄嘌呤氧化酶是治疗高尿酸血症药物的关键靶点,中药菊苣提取物具黄嘌呤氧化酶抑制作用,但作用机制仍未探明。该文通过检索相关文献及数据库收集菊苣已知小分子化合物建立配体库,从PDB数据库下载黄嘌呤氧化酶X-ray晶体三维结构文件,采用Autodock 4.2分子对接软件虚拟筛选出菊苣中的黄嘌呤氧化酶抑制剂。通过与原配体TEI(非布司他)相比较,筛选出70个与靶蛋白有较好结合作用的化合物。对接结果有助于阐释降尿酸作用机制,为菊苣治疗高尿酸血症作用机制研究提供一定的参考。 展开更多
关键词 分子对接 菊苣 黄嘌呤氧化酶 虚拟筛选
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Nutrients and Bioactive Molecules of the <i>Early</i>and <i>Late</i>Cultivars of the Treviso Red Chicory (<i>Cichorium intybus</i>L.)
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作者 Laura D’Evoli Massimo Lucarini +4 位作者 José Sanchez del Pulgar Altero Aguzzi Paolo Gabrielli Elena Azzini Ginevra Lombardi-Boccia 《Food and Nutrition Sciences》 2017年第5期457-464,共8页
The study provides original data on the compositional profile (macronutrients, dietary fiber, mineral and trace elements, bioactive molecules) of an Italian typical plant foods, Treviso Red Cichory, studying the two c... The study provides original data on the compositional profile (macronutrients, dietary fiber, mineral and trace elements, bioactive molecules) of an Italian typical plant foods, Treviso Red Cichory, studying the two cultivars (Early, Late) grown following two different traditional cultivation systems. For two consecutive years plants from three growing areas, were studied. Major, significant differences, between Early and Late cultivars, were observed in minerals (Ca, Mg, P), trace elements (Fe, Zn) and bioactive molecules content. The Late cv. was found the richest in minerals and trace elements content than the Early cv., but for Ca. Treviso Red Cichory was found a valuable source of bioactive molecules such as ascorbic acid, anthocyanins and total polyphenols. The Early cv. showed a significantly higher (p < 0.05) ascorbic acid and total anthocyanins content (8.63 and 92.15 mg/100g, respectively) than the Late cv. (6.15 and 24.38 mg/100g, respectively), by contrast total polyphenols content was significantly higher (p Late cv. A marked variability in bioactive molecules content among the growing areas was found only for the Late cv. The observed differences between the Early and Late cultivars could be strictly related to the traditional growing systems applied during plant’s growth. 展开更多
关键词 Treviso RED cichory Ascorbic Acid Anthocyanins POLYPHENOLS Minerals Dietary Fiber
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紫锥菊中菊苣酸的提取工艺和含量测定 被引量:9
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作者 钟英杰 李亮 徐福亮 《中国实验方剂学杂志》 CAS 北大核心 2010年第4期1-4,共4页
目的:优选紫锥菊药材地上部分的提取工艺,建立菊苣酸的含量测定方法。方法:择L9(34)正交表安排试验,以有效成分菊苣酸的含量为指标优选工艺参数。结果:紫锥菊的最佳提取工艺为12倍量60%乙醇提取3次,每次1.5 h。菊苣酸线性范围在0.011~1... 目的:优选紫锥菊药材地上部分的提取工艺,建立菊苣酸的含量测定方法。方法:择L9(34)正交表安排试验,以有效成分菊苣酸的含量为指标优选工艺参数。结果:紫锥菊的最佳提取工艺为12倍量60%乙醇提取3次,每次1.5 h。菊苣酸线性范围在0.011~1.100 mg,r=0.999 8,平均回收率99.51%(RSD=1.01%,n=6)。结论:工艺稳定、可行,适合工业化生产;含量测定方法简便准确,重复性好,可用于控制紫锥菊的质量。 展开更多
关键词 紫锥菊 菊苣酸 提取工艺 高效液相色谱法
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