Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs wer...Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.展开更多
Objective To elucidate the development of mapping and localization of susceptible genes on chromosomes to asthma related phenotypes. Data sources Published articles about susceptibility genes for asthma related phenot...Objective To elucidate the development of mapping and localization of susceptible genes on chromosomes to asthma related phenotypes. Data sources Published articles about susceptibility genes for asthma related phenotypes were selected using PubMed. Study selection Using methods of candidate gene positional clone and genome-wide scan with linkage and association analysis to determine the location in the genome of susceptibility genes to asthma and asthma related phenotypes. Results There are multiple regions in the genome harboring susceptibility genes to asthma and asthma related phenotypes, including chromosomes 5, 11, 12, 6, 2, 3, 13, 7, 14, 9, 19 and 17. Many of these regions contain candidate genes involved in asthma development and progression. Some susceptible genes may affect the phenotype expression or response to therapy. In addition, the interaction of multiple genes with the environment may contribute to the susceptibility to asthma. Conclusions As an essential step toward cloning the susceptible genes to asthma, fine mapping and localization on chromosomes are definitely needed. Novel powerful tools for gene discovery and the integration of genetics, biology and bioinformatics should be pursued.展开更多
【目的】本研究旨在获得家蚕Bombyx mori Bcl-2家族同源基因,并分析其在不同组织和发育阶段的时空表达模式及功能。【方法】用c DNA末端快速扩增方法(rapid amplification of c DNA ends,RACE)克隆家蚕Bcl-2家族基因Bm Buffy,利用SSR分...【目的】本研究旨在获得家蚕Bombyx mori Bcl-2家族同源基因,并分析其在不同组织和发育阶段的时空表达模式及功能。【方法】用c DNA末端快速扩增方法(rapid amplification of c DNA ends,RACE)克隆家蚕Bcl-2家族基因Bm Buffy,利用SSR分子标记连锁分析确定其染色体定位。同时,用RT-PCR和q PCR技术分析该基因在家蚕幼虫及变态期间不同组织中的表达。【结果】克隆获得全长家蚕Bcl-2家族同源基因Bm Buffy,证明该基因位于第4号染色体上,开放阅读框长879 bp,编码292 aa,预测其分子量大小为32.4 k Da,等电点为9.94,且第130-231位氨基酸之间存在1个Bcl-2_like Superfamily结构域。系统进化发育树表明,其与黑腹果蝇Drosophila melanogaster的Dm Buffy关系最近,氨基酸序列一致性为27%。Bm Buffy在幼虫组织中的表达结果显示,其在马氏管中表达量最高,而且在不同组织变态期的关键时间点均有明显变化。【结论】家蚕Bm Buffy具有Bcl-2家族典型结构域,Bm Buffy定位于第4号染色体上,Bm Buffy在家蚕变态期的组织生理变化中起到一定作用。本文为进一步研究家蚕Bcl-2家族基因的功能奠定基础。展开更多
基金supported by the National Natural Science Foundation of China (No.30830080 and 30800779)the Natural Science Foundation of Beijing (No.5072035)the Ministry of Science and Technology of China (No.2006AA10Z135,2008AA10Z143,2006CB102105 and 2009CB941604)
文摘Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.
文摘Objective To elucidate the development of mapping and localization of susceptible genes on chromosomes to asthma related phenotypes. Data sources Published articles about susceptibility genes for asthma related phenotypes were selected using PubMed. Study selection Using methods of candidate gene positional clone and genome-wide scan with linkage and association analysis to determine the location in the genome of susceptibility genes to asthma and asthma related phenotypes. Results There are multiple regions in the genome harboring susceptibility genes to asthma and asthma related phenotypes, including chromosomes 5, 11, 12, 6, 2, 3, 13, 7, 14, 9, 19 and 17. Many of these regions contain candidate genes involved in asthma development and progression. Some susceptible genes may affect the phenotype expression or response to therapy. In addition, the interaction of multiple genes with the environment may contribute to the susceptibility to asthma. Conclusions As an essential step toward cloning the susceptible genes to asthma, fine mapping and localization on chromosomes are definitely needed. Novel powerful tools for gene discovery and the integration of genetics, biology and bioinformatics should be pursued.
文摘【目的】本研究旨在获得家蚕Bombyx mori Bcl-2家族同源基因,并分析其在不同组织和发育阶段的时空表达模式及功能。【方法】用c DNA末端快速扩增方法(rapid amplification of c DNA ends,RACE)克隆家蚕Bcl-2家族基因Bm Buffy,利用SSR分子标记连锁分析确定其染色体定位。同时,用RT-PCR和q PCR技术分析该基因在家蚕幼虫及变态期间不同组织中的表达。【结果】克隆获得全长家蚕Bcl-2家族同源基因Bm Buffy,证明该基因位于第4号染色体上,开放阅读框长879 bp,编码292 aa,预测其分子量大小为32.4 k Da,等电点为9.94,且第130-231位氨基酸之间存在1个Bcl-2_like Superfamily结构域。系统进化发育树表明,其与黑腹果蝇Drosophila melanogaster的Dm Buffy关系最近,氨基酸序列一致性为27%。Bm Buffy在幼虫组织中的表达结果显示,其在马氏管中表达量最高,而且在不同组织变态期的关键时间点均有明显变化。【结论】家蚕Bm Buffy具有Bcl-2家族典型结构域,Bm Buffy定位于第4号染色体上,Bm Buffy在家蚕变态期的组织生理变化中起到一定作用。本文为进一步研究家蚕Bcl-2家族基因的功能奠定基础。
