Multilayers incorporated with stimuli-responsive substances by means of layer-by-layer(LbL) self-assembly are much attractive due to their advantages of stimuli-responsiveness and potential applications in different f...Multilayers incorporated with stimuli-responsive substances by means of layer-by-layer(LbL) self-assembly are much attractive due to their advantages of stimuli-responsiveness and potential applications in different fields. In this study, pyrenemethyl acrylate(PA) was synthesized, and was copolymerized with acrylic acid(AA) to obtain the amphiphilic and photodegradable P(PA-co-AA)polymers with a PA:AA molar ratio of 1.3:3, and an average molecular weight of 6.9 kDa and polydispersity index of 1.04. They formed micelles spontaneously when dispersed in aqueous solution with a size of 27.5 nm in a dry state and 136.6 nm in a wet state. The micelles were readily decomposed to form aggregates as a result of the cleavage of the pyrenemethyl ester bonds under UV-irradiation. UV-responsive micelles-containing multilayers were prepared by LbL self-assembly of the UV-responsive micelles and polyallylamine hydrochloride(PAH). UV-irradiation of the multilayers resulted in the decomposition of micelles, leading to larger surface roughness, and enhanced swelling ratio and wettability of the multilayers. In vitro culture of A549, HepG2 and endothelial cells showed significantly better adhesion at 4 h on the UV-illuminated multilayers, whereas the cell proliferation was not affected significantly until 5 d.展开更多
The continued evolution of bacterial and fungal species poses a significant difficulty for the treatment of disease of microbial origin. Given this situation, the prevention of transmission of such microbial diseases ...The continued evolution of bacterial and fungal species poses a significant difficulty for the treatment of disease of microbial origin. Given this situation, the prevention of transmission of such microbial diseases becomes of increasing importance. Efforts of this laboratory have been directed toward the destruction of microbial species on environmental surfaces as a prophylaxis toward infection, and we herein report on the efficacy of a system that demonstrates activity against both Gram-positive and Gram-negative bacteria, as well as fungi. We report specifically herein on the use of fabric materials so activated for the destruction of these microbial species, useful for a variety of surfaces within hospital and related settings wherein transmission of microbial disease is a major problem, while these approaches are also applicable for a variety of other types of surfaces.展开更多
The aim of this study was to investigate the inhibitory effect of heparin/fibronectin （Hep/Fn） complexes on neointimal hyperplasia following endovascular intervention. Hep/Fn complexes were immobilized onto titanium...The aim of this study was to investigate the inhibitory effect of heparin/fibronectin （Hep/Fn） complexes on neointimal hyperplasia following endovascular intervention. Hep/Fn complexes were immobilized onto titanium （Ti） surfaces, with subsequent X-ray photoelectron spectroscopy （XPS）, Toluidine Blue 0 （TBO） and immunohistochemistry methods were used to characterize surface properties. Smooth muscle cell （SMC） cultures were used to evaluate the effect of Hep/Fn complexes on SMC proliferation. Results showed that Hep/Fn complexes successfully immobilized onto Ti surfaces and resulted in an inhibition of SMC proliferation. This study suggests that Hep/Fn surface-immobilized biomaterials develop as a new generation of biomaterials to prevent neointimal hyperplasia, particularly for use in cardiovascular implants.展开更多
Pericytes have the potential to be developed as a cell therapy for the treatment of wounds;however,the efficacy of any cell therapy relies on the successful delivery of intact and functioning cells.Here,the effect of ...Pericytes have the potential to be developed as a cell therapy for the treatment of wounds;however,the efficacy of any cell therapy relies on the successful delivery of intact and functioning cells.Here,the effect of delivering pericytes on wound repair was assessed alongside the development of a surface-functionalized pericyte patch.Plasma polymerization(PP)was used to functionalize the surface of silicone patches with heptylamine(HA)or acrylic acid(AA)monomers.Human pericytes were subsequently delivered to murine excisional wounds by intradermal injection or using the pericyte-laden patches and the comparative effects on wound healing,inflammation and revascularization determined.The AA surface provided the superior transfer of the cells to de-epidermized dermis.Excisional murine wounds treated either with pericytes injected directly into the wound or with the pericyte-laden AA patches showed improved healing with decreased neutrophil infiltration and reduced numbers of macrophages in the wounds.Pericyte delivery also enhanced angiogenesis through a mechanism independent of VEGF signalling.Pericytes,when delivered to wounds,improved healing responses by dampening inflammation and promoting angiogenesis.Delivery of pericytes using PP-AA-functionalized patches was equally as effective as direct injection of pericytes into wounds.Pericyte-functionalized dressings may therefore be a clinically relevant approach for the treatment of wounds.展开更多
基金supported by the National Natural Science Foundation of China (21434006, 21374097)the National Key Research and Development Program of China (2016YFC1100403)+1 种基金the 111 Project of China (B16042)the Fundamental Research Funds for the Central Universities (2017XZZX001-03A, 2017XZZX008-05)
文摘Multilayers incorporated with stimuli-responsive substances by means of layer-by-layer(LbL) self-assembly are much attractive due to their advantages of stimuli-responsiveness and potential applications in different fields. In this study, pyrenemethyl acrylate(PA) was synthesized, and was copolymerized with acrylic acid(AA) to obtain the amphiphilic and photodegradable P(PA-co-AA)polymers with a PA:AA molar ratio of 1.3:3, and an average molecular weight of 6.9 kDa and polydispersity index of 1.04. They formed micelles spontaneously when dispersed in aqueous solution with a size of 27.5 nm in a dry state and 136.6 nm in a wet state. The micelles were readily decomposed to form aggregates as a result of the cleavage of the pyrenemethyl ester bonds under UV-irradiation. UV-responsive micelles-containing multilayers were prepared by LbL self-assembly of the UV-responsive micelles and polyallylamine hydrochloride(PAH). UV-irradiation of the multilayers resulted in the decomposition of micelles, leading to larger surface roughness, and enhanced swelling ratio and wettability of the multilayers. In vitro culture of A549, HepG2 and endothelial cells showed significantly better adhesion at 4 h on the UV-illuminated multilayers, whereas the cell proliferation was not affected significantly until 5 d.
文摘The continued evolution of bacterial and fungal species poses a significant difficulty for the treatment of disease of microbial origin. Given this situation, the prevention of transmission of such microbial diseases becomes of increasing importance. Efforts of this laboratory have been directed toward the destruction of microbial species on environmental surfaces as a prophylaxis toward infection, and we herein report on the efficacy of a system that demonstrates activity against both Gram-positive and Gram-negative bacteria, as well as fungi. We report specifically herein on the use of fabric materials so activated for the destruction of these microbial species, useful for a variety of surfaces within hospital and related settings wherein transmission of microbial disease is a major problem, while these approaches are also applicable for a variety of other types of surfaces.
基金supported by the financial support of Natural Science Research Program of Jiangsu Education Department(No.13KJB310014)Natural Science Foundation of Jiangsu Province(BK20140429)the Natural Science Foundation of Nantong University(No.14ZY015,No.13R23)
文摘The aim of this study was to investigate the inhibitory effect of heparin/fibronectin （Hep/Fn） complexes on neointimal hyperplasia following endovascular intervention. Hep/Fn complexes were immobilized onto titanium （Ti） surfaces, with subsequent X-ray photoelectron spectroscopy （XPS）, Toluidine Blue 0 （TBO） and immunohistochemistry methods were used to characterize surface properties. Smooth muscle cell （SMC） cultures were used to evaluate the effect of Hep/Fn complexes on SMC proliferation. Results showed that Hep/Fn complexes successfully immobilized onto Ti surfaces and resulted in an inhibition of SMC proliferation. This study suggests that Hep/Fn surface-immobilized biomaterials develop as a new generation of biomaterials to prevent neointimal hyperplasia, particularly for use in cardiovascular implants.
基金This research was supported by the Cell Therapy Manufacturing Cooperative Research Centre(CTM CRC).Additionally,H.M.T.was supported through an Australian Post-Graduate Award scholarship and a top-up scholarship from the Playford Trust.P.A.received a University of South Australia President’s Postgraduate Scholarship,and A.J.C.is supported by a National Health and Medical Research Council(NHMRC)Senior Research Fellowship(GNT#1102617).
文摘Pericytes have the potential to be developed as a cell therapy for the treatment of wounds;however,the efficacy of any cell therapy relies on the successful delivery of intact and functioning cells.Here,the effect of delivering pericytes on wound repair was assessed alongside the development of a surface-functionalized pericyte patch.Plasma polymerization(PP)was used to functionalize the surface of silicone patches with heptylamine(HA)or acrylic acid(AA)monomers.Human pericytes were subsequently delivered to murine excisional wounds by intradermal injection or using the pericyte-laden patches and the comparative effects on wound healing,inflammation and revascularization determined.The AA surface provided the superior transfer of the cells to de-epidermized dermis.Excisional murine wounds treated either with pericytes injected directly into the wound or with the pericyte-laden AA patches showed improved healing with decreased neutrophil infiltration and reduced numbers of macrophages in the wounds.Pericyte delivery also enhanced angiogenesis through a mechanism independent of VEGF signalling.Pericytes,when delivered to wounds,improved healing responses by dampening inflammation and promoting angiogenesis.Delivery of pericytes using PP-AA-functionalized patches was equally as effective as direct injection of pericytes into wounds.Pericyte-functionalized dressings may therefore be a clinically relevant approach for the treatment of wounds.
