AIM: The aim of the present study is to use immunohisto chemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis. METHODS:...AIM: The aim of the present study is to use immunohisto chemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis. METHODS: From November 1990 to December 1996, 47 patients were treated with preoperative radiation followed by radical esophagectomy. All patients were confirmed pathologically as suffering from squamous cell carcinoma. Immunohistochemical stain was done for PCNA, cyclinDl protein expression and DNA content analyzed by image cytometry. Kaplan-Meier method for single prognostic factor and log-rank test was used to test the significant difference. Cox stepwise regression model and prognosis index model were used for survival analysis with multiple prognostic factors. RESULTS: Radio-pathological change, T stage and N stage, as the traditional prognostic factors had statistical difference in 3-, 5- and 10-year survival rates. While, tumor cell proliferating marked PCNA, cyclinDl and DNA content served as independent prognostic factors of esophageal carcinoma. There was definitely an identity between the single and multiple factor analyses. PI was more accurate to evaluate the prognosis of esophageal carcinoma. CONCLUSION: It is possible that tumor cell proliferating marked PCNA, cyclinD1 and DNA content would become the endpoints for evaluating the prognosis of esophageal carcinoma.展开更多
AIM： To isolate putative pancreatic stem cells （PSCs） from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cyto...AIM： To isolate putative pancreatic stem cells （PSCs） from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cytometry. The potential for pancreatic lineage and the capability of β-cell differentiation in these PSCs were evaluated as well. METHODS： By using serum-free medium supplemented with essential growth factors, we attempted to isolate the putative PSCs which has been reported to express nestin and pdx-1. The MatrigelTM was employed to evaluate the differential capacity of isolated cells. Dithizone staining, insulin content/secretion measurement, and immunohistochemistry staining were used to monitor the differentiation. Fluorescence activated cell sorting （FACS） was used to detect the phenotypic markers of putative PSCs. RESULTS： A monolayer of spindle-like cells was cultivated. The putative PSCs expressed pdx-1 and nestin. They were also able to differentiate into insulin-, glucagon-, and somatostatin-positive cells. The spectrum of phenotypic markers in PSCs was investigated; a similarity was revealed when using human bone marrow-derived stem cells as the comparative experiment, such as CD29, CD44, CD49, CD50, CD51, CD62E, PDGFR-α, CD73 （SH2）, CD81, CD105（SH3）. CONCLUSION： In this study, we successfully isolated PSCs from adult human pancreatic duct by using serumfree medium. These PSCs not only expressed nestin and pdx-1 but also exhibited markers attributable to mesenchymal stem cells. Although work is needed to elucidate the role of these cells, the application of these PSCs might be therapeutic strategies for diabetes mellitus.展开更多
AIM： To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus （SCCE） in Iranian patients and compare our results with former reports by using proteomics. METHODS： Protein wa...AIM： To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus （SCCE） in Iranian patients and compare our results with former reports by using proteomics. METHODS： Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed. RESULTS： Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin （TMβ）, myosin light chain 2 （and its isoform）, myosin regulatory light chain 2, peroxyredoxin 2, annexin I and an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 （HSP70）, TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratin I, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ, HSP70, annexin Ⅰ, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers. CONCLUSION： Our study not only supports the involvement of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ.展开更多
Nestin is a class Ⅵ intermediate filament protein that was originally described as a neuronal stem cell marker during central nervous system (CNS) development, and is currently widely used in that capacity. Nestin is...Nestin is a class Ⅵ intermediate filament protein that was originally described as a neuronal stem cell marker during central nervous system (CNS) development, and is currently widely used in that capacity. Nestin is also expressed in non-neuronal immature or progenitor cells in normal tissues. Under pathological conditions, nestin is expressed in repair processes in the CNS, muscle, liver, and infarcted myocardium. Furthermore, increased nestin expression has been reported in various tumor cells, including CNS tumors, gastrointestinal stromal tumors, pancreatic cancer, prostate cancer, breast cancer, malignant melanoma, dermatofibrosarcoma protuberances, and thyroid tumors. Nestin is reported to correlate with aggressive growth, metastasis, and poor prognosis in some tumors; however, the roles of nestin in cancer cells have not been well characterized. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers. These findings indicate that nestin may be a marker for newly synthesized tumor vessels and a therapeutic target for tumor angiogenesis. It has received a lot of attention recently as a cancer stem cell marker in various cancer cells including brain tumors, malignant rhabdoid tumors, and uterine, cervical, prostate, bladder, head and neck, ovarian, testicular, and pancreatic cancers. The purpose of this review is to clarify the roles of nestin in cancer cells and in tumor angiogenesis, and to examine the association between nestin and cancer stem cells. Nestin has the potential to serve as a molecular target for cancers with nestin-positive cancer cells and nestin-positive tumor vasculature.展开更多
目的研究异黏蛋白(Metadherin,MTDH)、转酮醇酶样基因1(transketolase Like Protein 1,TKTL1)在喉癌组织中的表达及其与相关肿瘤标志物及癌细胞增殖活力的相关性。方法选取2013年1月~2016年12月于我科治疗的70例确诊为喉鳞状细胞癌(简...目的研究异黏蛋白(Metadherin,MTDH)、转酮醇酶样基因1(transketolase Like Protein 1,TKTL1)在喉癌组织中的表达及其与相关肿瘤标志物及癌细胞增殖活力的相关性。方法选取2013年1月~2016年12月于我科治疗的70例确诊为喉鳞状细胞癌(简称喉癌组)患者为研究对象,另选70例同期于我院诊断为声带息肉患者为对照组,测定癌组织和息肉组织中MTDH、TKTL1的阳性表达率及MTDH、TKTL1的mRNA表达量,检测两组患者血清鳞状细胞癌抗原(squamous cell carcinoma related antigen,SCCAg)、癌胚抗原(carcinoembryonic antigen,CEA)、糖类抗原199(carbohydrate antigen,CA199)含量,检测组织中癌基因信号传导及转录激活因子3(signal transducers and activators of transcription,STAT3)、原癌基因c-myc的mRNA含量,采用Pearson法检测MTDH、TKTL1与血清SCCAg、CEA、CA199及STAT3、c-myc表达量的相关性。结果喉癌组织中MTDH与TKTL1阳性率分别为58.57%、64.29%,均高于对照组的20%、12.86%(P均<0.01)。喉癌组MTDH与TKTL1的mRNA表达量分别为1.78±0.45、1.97±0.62,均显著高于对照组的0.19±0.03、0.21±0.07(P均<0.01);喉癌组的血清肿瘤标志物SCCAg、CEA、CA199水平分别为6.75±2.74、9.36±2.73、31.54±10.36,显著高于对照组的1.34±0.83、3.21±1.36、12.42±4.13(P均<0.01);喉癌组织中的STAT3、c-myc mRNA表达量分别为2.36±0.75、1.99±0.51,显著高于对照组的0.67±0.12、0.71±0.15(P均<0.01)。M T DH、T KT L1表达量与血清SCCAg、CEA、CA199含量及STAT3、c-myc均呈正相关。结论 MTDH、TKTL1在喉癌组织中的表达显著增加,与癌症发生发展密切相关,且与肿瘤标志物、促癌基因的表达呈正相关,有促进癌细胞增殖的作用。展开更多
The paper reported various antibiotic sensitivity test of somatic cells of Porphyra yezoensis. The result showed somatic cells are very sensitive to Chloromycetin; semicide dose is less than 100 U/ml. It is proved tha...The paper reported various antibiotic sensitivity test of somatic cells of Porphyra yezoensis. The result showed somatic cells are very sensitive to Chloromycetin; semicide dose is less than 100 U/ml. It is proved that Chloromycetin is a good selective pressure for gene transformation of P. yezoensis. Somatic cells are also sensitive to tetracin.展开更多
侧群细胞(side population cell)是利用Hoechst染料和流式细胞术进行造血干/祖细胞分离时发现的一群特殊细胞,广泛分布于多种成体组织、胚胎和某些肿瘤细胞系中;它既具有类似干细胞的自我更新和多向分化潜能,还具有独特的表型标记和生...侧群细胞(side population cell)是利用Hoechst染料和流式细胞术进行造血干/祖细胞分离时发现的一群特殊细胞,广泛分布于多种成体组织、胚胎和某些肿瘤细胞系中;它既具有类似干细胞的自我更新和多向分化潜能,还具有独特的表型标记和生物学特征,代表了一种新的干细胞类型。对侧群细胞的研究,不仅有助于人们增加对干细胞增殖、分化及其发育调控机制的理解,同时还提供了一种从不同组织中分离纯化和利用多能干细胞的新策略,为组织工程和细胞治疗提供新的干细胞材料来源。现就侧群细胞的组织分布、生物学特征、表型标记、信号转导机制及其与肿瘤发生相关性等方面的研究进展进行了综述,并对侧群细胞的进一步研究和应用作了展望。展开更多
基金Supported by the Distinguished Young Teacher Programs Foundation of Ministry of Education of China, No. 2001125
文摘AIM: The aim of the present study is to use immunohisto chemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis. METHODS: From November 1990 to December 1996, 47 patients were treated with preoperative radiation followed by radical esophagectomy. All patients were confirmed pathologically as suffering from squamous cell carcinoma. Immunohistochemical stain was done for PCNA, cyclinDl protein expression and DNA content analyzed by image cytometry. Kaplan-Meier method for single prognostic factor and log-rank test was used to test the significant difference. Cox stepwise regression model and prognosis index model were used for survival analysis with multiple prognostic factors. RESULTS: Radio-pathological change, T stage and N stage, as the traditional prognostic factors had statistical difference in 3-, 5- and 10-year survival rates. While, tumor cell proliferating marked PCNA, cyclinDl and DNA content served as independent prognostic factors of esophageal carcinoma. There was definitely an identity between the single and multiple factor analyses. PI was more accurate to evaluate the prognosis of esophageal carcinoma. CONCLUSION: It is possible that tumor cell proliferating marked PCNA, cyclinD1 and DNA content would become the endpoints for evaluating the prognosis of esophageal carcinoma.
基金Supported by National Science Council, Yen-Tj ing-Ling Medical Foundation and Taipei Veterans General Hospital
文摘AIM： To isolate putative pancreatic stem cells （PSCs） from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cytometry. The potential for pancreatic lineage and the capability of β-cell differentiation in these PSCs were evaluated as well. METHODS： By using serum-free medium supplemented with essential growth factors, we attempted to isolate the putative PSCs which has been reported to express nestin and pdx-1. The MatrigelTM was employed to evaluate the differential capacity of isolated cells. Dithizone staining, insulin content/secretion measurement, and immunohistochemistry staining were used to monitor the differentiation. Fluorescence activated cell sorting （FACS） was used to detect the phenotypic markers of putative PSCs. RESULTS： A monolayer of spindle-like cells was cultivated. The putative PSCs expressed pdx-1 and nestin. They were also able to differentiate into insulin-, glucagon-, and somatostatin-positive cells. The spectrum of phenotypic markers in PSCs was investigated; a similarity was revealed when using human bone marrow-derived stem cells as the comparative experiment, such as CD29, CD44, CD49, CD50, CD51, CD62E, PDGFR-α, CD73 （SH2）, CD81, CD105（SH3）. CONCLUSION： In this study, we successfully isolated PSCs from adult human pancreatic duct by using serumfree medium. These PSCs not only expressed nestin and pdx-1 but also exhibited markers attributable to mesenchymal stem cells. Although work is needed to elucidate the role of these cells, the application of these PSCs might be therapeutic strategies for diabetes mellitus.
基金Supported by National Institute of Genetic Engineering and Biotechnology, No. 199 and Digestive Disease Research Center, No. 18/81
文摘AIM： To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus （SCCE） in Iranian patients and compare our results with former reports by using proteomics. METHODS： Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed. RESULTS： Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin （TMβ）, myosin light chain 2 （and its isoform）, myosin regulatory light chain 2, peroxyredoxin 2, annexin I and an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 （HSP70）, TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratin I, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ, HSP70, annexin Ⅰ, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers. CONCLUSION： Our study not only supports the involvement of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ.
基金Supported by Grants (No. S0801035, to Naito Z) from the Ministry of Education, Culture, Sports, Science, and Technol-ogy (MEXT), JapanGrant-in-Aid for Young Scientists (A, No. 22689038 to Matsuda Y)
文摘Nestin is a class Ⅵ intermediate filament protein that was originally described as a neuronal stem cell marker during central nervous system (CNS) development, and is currently widely used in that capacity. Nestin is also expressed in non-neuronal immature or progenitor cells in normal tissues. Under pathological conditions, nestin is expressed in repair processes in the CNS, muscle, liver, and infarcted myocardium. Furthermore, increased nestin expression has been reported in various tumor cells, including CNS tumors, gastrointestinal stromal tumors, pancreatic cancer, prostate cancer, breast cancer, malignant melanoma, dermatofibrosarcoma protuberances, and thyroid tumors. Nestin is reported to correlate with aggressive growth, metastasis, and poor prognosis in some tumors; however, the roles of nestin in cancer cells have not been well characterized. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers. These findings indicate that nestin may be a marker for newly synthesized tumor vessels and a therapeutic target for tumor angiogenesis. It has received a lot of attention recently as a cancer stem cell marker in various cancer cells including brain tumors, malignant rhabdoid tumors, and uterine, cervical, prostate, bladder, head and neck, ovarian, testicular, and pancreatic cancers. The purpose of this review is to clarify the roles of nestin in cancer cells and in tumor angiogenesis, and to examine the association between nestin and cancer stem cells. Nestin has the potential to serve as a molecular target for cancers with nestin-positive cancer cells and nestin-positive tumor vasculature.
文摘目的研究异黏蛋白(Metadherin,MTDH)、转酮醇酶样基因1(transketolase Like Protein 1,TKTL1)在喉癌组织中的表达及其与相关肿瘤标志物及癌细胞增殖活力的相关性。方法选取2013年1月~2016年12月于我科治疗的70例确诊为喉鳞状细胞癌(简称喉癌组)患者为研究对象,另选70例同期于我院诊断为声带息肉患者为对照组,测定癌组织和息肉组织中MTDH、TKTL1的阳性表达率及MTDH、TKTL1的mRNA表达量,检测两组患者血清鳞状细胞癌抗原(squamous cell carcinoma related antigen,SCCAg)、癌胚抗原(carcinoembryonic antigen,CEA)、糖类抗原199(carbohydrate antigen,CA199)含量,检测组织中癌基因信号传导及转录激活因子3(signal transducers and activators of transcription,STAT3)、原癌基因c-myc的mRNA含量,采用Pearson法检测MTDH、TKTL1与血清SCCAg、CEA、CA199及STAT3、c-myc表达量的相关性。结果喉癌组织中MTDH与TKTL1阳性率分别为58.57%、64.29%,均高于对照组的20%、12.86%(P均<0.01)。喉癌组MTDH与TKTL1的mRNA表达量分别为1.78±0.45、1.97±0.62,均显著高于对照组的0.19±0.03、0.21±0.07(P均<0.01);喉癌组的血清肿瘤标志物SCCAg、CEA、CA199水平分别为6.75±2.74、9.36±2.73、31.54±10.36,显著高于对照组的1.34±0.83、3.21±1.36、12.42±4.13(P均<0.01);喉癌组织中的STAT3、c-myc mRNA表达量分别为2.36±0.75、1.99±0.51,显著高于对照组的0.67±0.12、0.71±0.15(P均<0.01)。M T DH、T KT L1表达量与血清SCCAg、CEA、CA199含量及STAT3、c-myc均呈正相关。结论 MTDH、TKTL1在喉癌组织中的表达显著增加,与癌症发生发展密切相关,且与肿瘤标志物、促癌基因的表达呈正相关,有促进癌细胞增殖的作用。
文摘The paper reported various antibiotic sensitivity test of somatic cells of Porphyra yezoensis. The result showed somatic cells are very sensitive to Chloromycetin; semicide dose is less than 100 U/ml. It is proved that Chloromycetin is a good selective pressure for gene transformation of P. yezoensis. Somatic cells are also sensitive to tetracin.
文摘侧群细胞(side population cell)是利用Hoechst染料和流式细胞术进行造血干/祖细胞分离时发现的一群特殊细胞,广泛分布于多种成体组织、胚胎和某些肿瘤细胞系中;它既具有类似干细胞的自我更新和多向分化潜能,还具有独特的表型标记和生物学特征,代表了一种新的干细胞类型。对侧群细胞的研究,不仅有助于人们增加对干细胞增殖、分化及其发育调控机制的理解,同时还提供了一种从不同组织中分离纯化和利用多能干细胞的新策略,为组织工程和细胞治疗提供新的干细胞材料来源。现就侧群细胞的组织分布、生物学特征、表型标记、信号转导机制及其与肿瘤发生相关性等方面的研究进展进行了综述,并对侧群细胞的进一步研究和应用作了展望。
