Objective:To assess and characterize antibiotic resistance in Acinetobacter baumannii strains recovered from 5 health-care facilities in Algiers.Methods:Antibiotic susceptibility testing was performed by agar diffusio...Objective:To assess and characterize antibiotic resistance in Acinetobacter baumannii strains recovered from 5 health-care facilities in Algiers.Methods:Antibiotic susceptibility testing was performed by agar diffusion and agar dilution methods,resistance genes were identified by PCR and sequencing,and molecular typing of isolates was carried out by enterobacterial repetitive intergenic consensus-PCR(ERIC-PCR).Results:Among 125 tested isolates,117(93.6% ) were multidrug-resistant.of which 94(75.2% ) were imipenem resistant.The bla_(ADC)and bla_(OXA-51-like) genes were detected in all isolates,in association with ISAba I sequence in 84% and 8% (imipenem resistant) of isolates,respectively.The bla_(OXA-23-like) and bla_(OXA-24-like)carbapenemase genes were delected in 67.02% and 20.21% of imipenem-resistant isolates,respectively.The bla_(OXA-23-like) gene is linked to ISAba1 or ISAba4 elements.The metallo-β-lactamase NDM-1 gene was found in 10(10.6% ) imipenem-resisianl strains from three hospitals,it is linked to ISAba125 clement in nine strains.Extended spectrum β-lactamases production was not detected.Imipenem and cefotaxime resistance phenolypes could not be transferred to Escherichia coli by conjugation.Outer membrane protein CarO gene was not delected in four imipenem-resisianl isolates.The aac(6')-1b.sul1,sul2,tetA and tetB genes were present in 5.31% .36.17% .77.65% .1.06% and 65.92% of strains,respectively.Class 1 integrons were detected in 23.4% strains.KRIC-PCR typing showed a genetic diversity among bla_(OXA-23-like) and bla_(OXA-24-like) positive strains,while clonality was observed among bla_(NDM-1)positives.Conclusions:This study highlighted the high prevalence of imipenem resistance in Acinetobacter baumannii in Algiers hospitals mediated mainly by bla_(OXA-23-like),bla_(OXA-24-like),and bla_(NDM-1) genes.展开更多
Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into caro...Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into carotenes and xanthophylls on the basis of their functional groups(Saini et al.,2015).Carotenes can be lin‐ear(phytoene,phytofluene,andζ-carotene)or branched(β-carotene andα-carotene).Xanthophylls compriseβ,β-xanthophylls(β-cryptoxanthin,zeaxanthin,violaxanthins,and neoxanthin)andβ,ε-xanthophylls(α-cryptoxanthin,α-carotene,and lutein).展开更多
Neutron background measurement is always very important for dark matter detection due to almost the same effect for the recoiled nucleus scattered off by the incident neutron and dark matter particle. For deep under-g...Neutron background measurement is always very important for dark matter detection due to almost the same effect for the recoiled nucleus scattered off by the incident neutron and dark matter particle. For deep under-ground experiments, the flux of neutron background is so low that large-scale detection is usually necessary. In this paper, by using Geant4, the relationship between detection efficiency and volume is investigated, meanwhile, two geometrical schemes for this detection including a single large-sized detector and arrayed multi-detector are compared under the condition of the same volume. The geometrical parameters of detectors are filtrated and detection efficiencies obtained under the similar background condition of China Jingping Underground Laboratory （CJPL）. The results show that for a large-scale Gd-doped liquid scintillation detector, the detection efficiency increases with the size of detector at the beginning and then trends toward a constant. Under the condition of the same length and cross section, the arrayed multi-detector has almost similar detection performance as the single large-sized detector, while too much detector number could cause degeneration of detection performance. Considering engineering factors, such as testing, assembling and production, the 4 × 4 arrayed detector scheme is flexible and more suitable. Furthermore, the conditions for using fast and slow signal coincidence detection and the detectable lower limit of neutron energy are evaluated by simulating the light process.展开更多
基金supported by grants from National Fund for the Research and National Agency for the Development of Research in Health(Algeria)
文摘Objective:To assess and characterize antibiotic resistance in Acinetobacter baumannii strains recovered from 5 health-care facilities in Algiers.Methods:Antibiotic susceptibility testing was performed by agar diffusion and agar dilution methods,resistance genes were identified by PCR and sequencing,and molecular typing of isolates was carried out by enterobacterial repetitive intergenic consensus-PCR(ERIC-PCR).Results:Among 125 tested isolates,117(93.6% ) were multidrug-resistant.of which 94(75.2% ) were imipenem resistant.The bla_(ADC)and bla_(OXA-51-like) genes were detected in all isolates,in association with ISAba I sequence in 84% and 8% (imipenem resistant) of isolates,respectively.The bla_(OXA-23-like) and bla_(OXA-24-like)carbapenemase genes were delected in 67.02% and 20.21% of imipenem-resistant isolates,respectively.The bla_(OXA-23-like) gene is linked to ISAba1 or ISAba4 elements.The metallo-β-lactamase NDM-1 gene was found in 10(10.6% ) imipenem-resisianl strains from three hospitals,it is linked to ISAba125 clement in nine strains.Extended spectrum β-lactamases production was not detected.Imipenem and cefotaxime resistance phenolypes could not be transferred to Escherichia coli by conjugation.Outer membrane protein CarO gene was not delected in four imipenem-resisianl isolates.The aac(6')-1b.sul1,sul2,tetA and tetB genes were present in 5.31% .36.17% .77.65% .1.06% and 65.92% of strains,respectively.Class 1 integrons were detected in 23.4% strains.KRIC-PCR typing showed a genetic diversity among bla_(OXA-23-like) and bla_(OXA-24-like) positive strains,while clonality was observed among bla_(NDM-1)positives.Conclusions:This study highlighted the high prevalence of imipenem resistance in Acinetobacter baumannii in Algiers hospitals mediated mainly by bla_(OXA-23-like),bla_(OXA-24-like),and bla_(NDM-1) genes.
基金This work was supported by the National Key Research and Development Program of China(No.2021YFD1600802-02)。
文摘Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into carotenes and xanthophylls on the basis of their functional groups(Saini et al.,2015).Carotenes can be lin‐ear(phytoene,phytofluene,andζ-carotene)or branched(β-carotene andα-carotene).Xanthophylls compriseβ,β-xanthophylls(β-cryptoxanthin,zeaxanthin,violaxanthins,and neoxanthin)andβ,ε-xanthophylls(α-cryptoxanthin,α-carotene,and lutein).
基金Supported by National Natural Science Foundation of China (10935005, 10945002)
文摘Neutron background measurement is always very important for dark matter detection due to almost the same effect for the recoiled nucleus scattered off by the incident neutron and dark matter particle. For deep under-ground experiments, the flux of neutron background is so low that large-scale detection is usually necessary. In this paper, by using Geant4, the relationship between detection efficiency and volume is investigated, meanwhile, two geometrical schemes for this detection including a single large-sized detector and arrayed multi-detector are compared under the condition of the same volume. The geometrical parameters of detectors are filtrated and detection efficiencies obtained under the similar background condition of China Jingping Underground Laboratory （CJPL）. The results show that for a large-scale Gd-doped liquid scintillation detector, the detection efficiency increases with the size of detector at the beginning and then trends toward a constant. Under the condition of the same length and cross section, the arrayed multi-detector has almost similar detection performance as the single large-sized detector, while too much detector number could cause degeneration of detection performance. Considering engineering factors, such as testing, assembling and production, the 4 × 4 arrayed detector scheme is flexible and more suitable. Furthermore, the conditions for using fast and slow signal coincidence detection and the detectable lower limit of neutron energy are evaluated by simulating the light process.
