Objective:To investigate the antibacterial and cytotoxic activity of fourteen different edible vegetables methanolic extract from Bangladesh.Methods:The antibacterial activity was evaluated using disc diffusion assay ...Objective:To investigate the antibacterial and cytotoxic activity of fourteen different edible vegetables methanolic extract from Bangladesh.Methods:The antibacterial activity was evaluated using disc diffusion assay method against 12 bacteria(both gram positive and gram negative).The plant extracts were also screened for cytotoxic activity using the brine shrimp lethality bioassay method and the lethal concentrations(LC_(50))were determined at confidence intervals by analyzing the data on a computer loaded with"Finney Programme??Results:All the vegetable extracts showed low to elevated levels of antibacterial activity against most of the tested strains(zone of inhibition=5-28 mm).The most active extract against all bacterial strains was from Xanthium indicum which showed remarkable antibacterial activity having the diameter of growth inhibition zone ranging from 12 to 28 mm followed by Alternanthera sessilis(zone of inhibition=6-21 mm).All extracts exhibited considerable general toxicity towards brine shrimps.The LC_(50)value of the tested extracts was within the range of 8.447 to 60.323μg/mL with respect to the positive control(vincristine sulphate)which was 0.91μg/mL.Among all studied extracts,Xanthium indicum displayed the highest cytotoxic effect with LC_(50)value of 8.447μg/mL.Conclusions:The results of the present investigation suggest that most of the studied plants are potentially good source of antibacterial and anticancer agents.展开更多
Objective:To study the biological activities of the tissue extract of Cantharus tranquebaricus(C.tranquebaricus).Methods:Crude extract of gastropod was tested for inhibition of bacterial growth.Antibacterial assay was...Objective:To study the biological activities of the tissue extract of Cantharus tranquebaricus(C.tranquebaricus).Methods:Crude extract of gastropod was tested for inhibition of bacterial growth.Antibacterial assay was carried out by disc diffusion method and the activity was measured accordingly based on the inhibition zone around the disc impregnated with gastropod extract.Molecular weight of the extract was determined by using SDS-PAGE.Plasma coagulation,Fibrin plate assay and substrate SDS-PAGE were used to determine the effect of sample on plasma coagulation,fibrin(ogen)olytic and proteolytic;activity.Results:The maximum inhibition zone(10 mm)was observed against Vibrio cholera(V.cholera)and minimum inhibition zone(2 mm)was noticed against Proteus mirablis(P.mirablis).The molecular weight was determined as 47-106kDa.The tissue extract shows proteolytic activity above 48 kDa.SDS-PAGE analysis of fibrinogen after incubation with the tissue extract showed fibrinogenolytic activity.In plasma coagulation assay C.tranquebaricus tissue extract showed procoagulant property and it coagulated chicken plasma within 150 s,while control took 5 min to clot.The 9 HU hemolytic units were found against chicken blood and also exhibit high level of brine shrimp lethality.Conclusions:This study suggests that C.tranquebaricus could be used as potential source for isolating bioactive compounds,since it is explored first time and found with promising results.展开更多
【背景】萘并吡喃酮类化合物生物活性多样,是真菌Aspergillusniger中特征次生代谢产物。【目的】研究分离自海洋滩涂土壤的真菌Aspergillus niger XJJ-3中萘并吡喃酮类化合物结构及其抗菌和卤虫致死活性。【方法】以TLC分析为导向,综合...【背景】萘并吡喃酮类化合物生物活性多样,是真菌Aspergillusniger中特征次生代谢产物。【目的】研究分离自海洋滩涂土壤的真菌Aspergillus niger XJJ-3中萘并吡喃酮类化合物结构及其抗菌和卤虫致死活性。【方法】以TLC分析为导向,综合运用多种色谱和光谱方法分离和鉴定萘并吡喃酮类化合物。采用微量稀释法测试化合物的抗菌和卤虫致死活性。【结果】从真菌A.niger大米发酵产物中共分离得到6个萘并吡喃酮类化合物,鉴定为RubrofusarinB(1)、Flavasperone (2)、Aurasperone A (3)、Asperpyrones C (4)、Asperpyrones B (5)和Fonsecinone A (6)。抗菌活性实验结果表明,化合物1-6对致病菌S. aureus ATCC33591、29213、E. faecium ATCC35667和V.parahemolyticus表现出不同程度的抑制活性,其中化合物2和4对S.aureus ATCC33591表现出较强抑制活性(MIC分别为43.7μmol/L和21.9μmol/L),化合物3对E.faecium ATCC35667抑制活性较强(MIC为21.9μmol/L)。卤虫致死活性实验结果表明,化合物1-6均表现出一定的卤虫致死活性,其中化合物2和3活性显著(LD50分别为35.0μmol/L和8.8μmol/L)。【结论】菌株XJJ-3可产生结构丰富的萘并吡喃酮类化合物,化合物1-6存在不同程度的抗菌和卤虫致死活性,该研究可为抗菌和细胞毒类药物的研发提供参考。展开更多
<span style='font-family:Verdana;font-size:12px;'>The medicinal uses and applications of metal complexes are of increasing clinical and commercial importance;this is as a result of some level of success ...<span style='font-family:Verdana;font-size:12px;'>The medicinal uses and applications of metal complexes are of increasing clinical and commercial importance;this is as a result of some level of success </span><span style='font-family:Verdana;font-size:12px;'>achieved so far. In this regard, novel enamine free-base ligands were synthesized</span><span style='font-family:Verdana;font-size:12px;'> by the condensation of terephthalaldehyde and 2-(methylamino)ethanol. This afforded a dinegative </span><i><span style='font-family:Verdana;font-size:12px;'>ONNO</span></i><span style='font-family:Verdana;font-size:12px;'> donor enamine, free base, characterized using </span><sup><span style='font-family:Verdana;font-size:12px;'>1</span></sup><span style='font-family:Verdana;font-size:12px;'>H and </span><sup><span style='font-family:Verdana;font-size:12px;'>13</span></sup><span style='font-family:Verdana;font-size:12px;'>C NMR, Fourier-transformed infrared and UV-vis spectroscopy. Coordination compounds of the enamine were also synthesized using Cu(II), Ni(II), Co(II) and VO(IV) ions. These complexes were characterized by electronic, IR spectrophotometry, mass spectrometry, magnetic susceptibility and </span><span style='font-family:Verdana;font-size:12px;'>E</span><span style='font-family:Verdana;font-size:12px;'>DX. The compounds were thereafter evaluated for their antimicrobial and cytotoxic activities. The data obtained w</span><span style='font-size:12px;font-family:Verdana;'>ere</span><span style='font-size:12px;font-family:Verdana;'> supportive of a</span><span style='font-size:12px;font-family:Verdana;'>n</span><span style='font-size:10.0pt;font-family:''><span style='font-family:Verdana;font-size:12px;'> octahedral geometry</span><span style='font-family:Verdana;font-size:12px;'> for the Cu(II) complex, a square-pyramidal geometry for the vanadium complex and a 4-coordinate square-planar geometry for both the cobalt and nickel complexes. The magnetic susceptibility data revealed that the complexes are magnetical展开更多
Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of C...Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD1-12). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy.?Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC50 values 3.79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC50 value 30.52 μg/ml), CD11 (IC50 value 39.27 μg/ml) and CD12 (IC50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.展开更多
文摘Objective:To investigate the antibacterial and cytotoxic activity of fourteen different edible vegetables methanolic extract from Bangladesh.Methods:The antibacterial activity was evaluated using disc diffusion assay method against 12 bacteria(both gram positive and gram negative).The plant extracts were also screened for cytotoxic activity using the brine shrimp lethality bioassay method and the lethal concentrations(LC_(50))were determined at confidence intervals by analyzing the data on a computer loaded with"Finney Programme??Results:All the vegetable extracts showed low to elevated levels of antibacterial activity against most of the tested strains(zone of inhibition=5-28 mm).The most active extract against all bacterial strains was from Xanthium indicum which showed remarkable antibacterial activity having the diameter of growth inhibition zone ranging from 12 to 28 mm followed by Alternanthera sessilis(zone of inhibition=6-21 mm).All extracts exhibited considerable general toxicity towards brine shrimps.The LC_(50)value of the tested extracts was within the range of 8.447 to 60.323μg/mL with respect to the positive control(vincristine sulphate)which was 0.91μg/mL.Among all studied extracts,Xanthium indicum displayed the highest cytotoxic effect with LC_(50)value of 8.447μg/mL.Conclusions:The results of the present investigation suggest that most of the studied plants are potentially good source of antibacterial and anticancer agents.
基金grateful to UGC[G4/9493]and MoES[G4/12319]for providing financial support
文摘Objective:To study the biological activities of the tissue extract of Cantharus tranquebaricus(C.tranquebaricus).Methods:Crude extract of gastropod was tested for inhibition of bacterial growth.Antibacterial assay was carried out by disc diffusion method and the activity was measured accordingly based on the inhibition zone around the disc impregnated with gastropod extract.Molecular weight of the extract was determined by using SDS-PAGE.Plasma coagulation,Fibrin plate assay and substrate SDS-PAGE were used to determine the effect of sample on plasma coagulation,fibrin(ogen)olytic and proteolytic;activity.Results:The maximum inhibition zone(10 mm)was observed against Vibrio cholera(V.cholera)and minimum inhibition zone(2 mm)was noticed against Proteus mirablis(P.mirablis).The molecular weight was determined as 47-106kDa.The tissue extract shows proteolytic activity above 48 kDa.SDS-PAGE analysis of fibrinogen after incubation with the tissue extract showed fibrinogenolytic activity.In plasma coagulation assay C.tranquebaricus tissue extract showed procoagulant property and it coagulated chicken plasma within 150 s,while control took 5 min to clot.The 9 HU hemolytic units were found against chicken blood and also exhibit high level of brine shrimp lethality.Conclusions:This study suggests that C.tranquebaricus could be used as potential source for isolating bioactive compounds,since it is explored first time and found with promising results.
文摘【背景】萘并吡喃酮类化合物生物活性多样,是真菌Aspergillusniger中特征次生代谢产物。【目的】研究分离自海洋滩涂土壤的真菌Aspergillus niger XJJ-3中萘并吡喃酮类化合物结构及其抗菌和卤虫致死活性。【方法】以TLC分析为导向,综合运用多种色谱和光谱方法分离和鉴定萘并吡喃酮类化合物。采用微量稀释法测试化合物的抗菌和卤虫致死活性。【结果】从真菌A.niger大米发酵产物中共分离得到6个萘并吡喃酮类化合物,鉴定为RubrofusarinB(1)、Flavasperone (2)、Aurasperone A (3)、Asperpyrones C (4)、Asperpyrones B (5)和Fonsecinone A (6)。抗菌活性实验结果表明,化合物1-6对致病菌S. aureus ATCC33591、29213、E. faecium ATCC35667和V.parahemolyticus表现出不同程度的抑制活性,其中化合物2和4对S.aureus ATCC33591表现出较强抑制活性(MIC分别为43.7μmol/L和21.9μmol/L),化合物3对E.faecium ATCC35667抑制活性较强(MIC为21.9μmol/L)。卤虫致死活性实验结果表明,化合物1-6均表现出一定的卤虫致死活性,其中化合物2和3活性显著(LD50分别为35.0μmol/L和8.8μmol/L)。【结论】菌株XJJ-3可产生结构丰富的萘并吡喃酮类化合物,化合物1-6存在不同程度的抗菌和卤虫致死活性,该研究可为抗菌和细胞毒类药物的研发提供参考。
文摘<span style='font-family:Verdana;font-size:12px;'>The medicinal uses and applications of metal complexes are of increasing clinical and commercial importance;this is as a result of some level of success </span><span style='font-family:Verdana;font-size:12px;'>achieved so far. In this regard, novel enamine free-base ligands were synthesized</span><span style='font-family:Verdana;font-size:12px;'> by the condensation of terephthalaldehyde and 2-(methylamino)ethanol. This afforded a dinegative </span><i><span style='font-family:Verdana;font-size:12px;'>ONNO</span></i><span style='font-family:Verdana;font-size:12px;'> donor enamine, free base, characterized using </span><sup><span style='font-family:Verdana;font-size:12px;'>1</span></sup><span style='font-family:Verdana;font-size:12px;'>H and </span><sup><span style='font-family:Verdana;font-size:12px;'>13</span></sup><span style='font-family:Verdana;font-size:12px;'>C NMR, Fourier-transformed infrared and UV-vis spectroscopy. Coordination compounds of the enamine were also synthesized using Cu(II), Ni(II), Co(II) and VO(IV) ions. These complexes were characterized by electronic, IR spectrophotometry, mass spectrometry, magnetic susceptibility and </span><span style='font-family:Verdana;font-size:12px;'>E</span><span style='font-family:Verdana;font-size:12px;'>DX. The compounds were thereafter evaluated for their antimicrobial and cytotoxic activities. The data obtained w</span><span style='font-size:12px;font-family:Verdana;'>ere</span><span style='font-size:12px;font-family:Verdana;'> supportive of a</span><span style='font-size:12px;font-family:Verdana;'>n</span><span style='font-size:10.0pt;font-family:''><span style='font-family:Verdana;font-size:12px;'> octahedral geometry</span><span style='font-family:Verdana;font-size:12px;'> for the Cu(II) complex, a square-pyramidal geometry for the vanadium complex and a 4-coordinate square-planar geometry for both the cobalt and nickel complexes. The magnetic susceptibility data revealed that the complexes are magnetical
文摘Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD1-12). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy.?Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC50 values 3.79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC50 value 30.52 μg/ml), CD11 (IC50 value 39.27 μg/ml) and CD12 (IC50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.
