BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarco...BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.展开更多
目的探讨在骨肿瘤疾病临床教学中应用多学科综合诊疗(multidiciplinary treatment,MDT)模式下PBL教学法的教学效果。方法选取新疆医科大学五年制医学影像专业在新疆医科大学附属肿瘤医院进行骨肿瘤疾病临床示教学生共75名,按班级分为实...目的探讨在骨肿瘤疾病临床教学中应用多学科综合诊疗(multidiciplinary treatment,MDT)模式下PBL教学法的教学效果。方法选取新疆医科大学五年制医学影像专业在新疆医科大学附属肿瘤医院进行骨肿瘤疾病临床示教学生共75名,按班级分为实验组(MDT模式下PBL组)和对照组(传统教学LBL组),分别按照各组教学方式进行临床示教,比较2组教学效果和学生满意度的差别。结果 (1)MDT模式下PBL组理论成绩与对照组相比,差异无统计学意义(43.13±4.12 vs 42.76±3.05,P>0.05),但临床思维成绩实验组高于对照组,差异有统计学意义(40.28±3.52 vs 36.13±2.88,P<0.05);(2)在活跃课堂气氛、提高自主学习能力、提高语言表达能力、提高沟通交往能力、提升自信心方面,实验组的满意度高于对照组,差异有统计学意义(P<0.05)。结论 MDT模式下PBL教学法在骨肿瘤疾病临床教学中效果良好,学生满意度较高,符合现代医学教育理念,可以在骨肿瘤临床教学中推广。展开更多
目的探讨案例教学法联合多学科协作的教学模式在骨肿瘤临床教学中的应用效果。方法选取2015年1月至2016年12月在我院接受住院医师规范化培训的50名学员作为教学对象,根据所在的病区不同分为研究组(22例)和对照组(28例),研究组采用案例教...目的探讨案例教学法联合多学科协作的教学模式在骨肿瘤临床教学中的应用效果。方法选取2015年1月至2016年12月在我院接受住院医师规范化培训的50名学员作为教学对象,根据所在的病区不同分为研究组(22例)和对照组(28例),研究组采用案例教学(case based learning,CBL)联合多学科协作(multidisciplinary teamwork,MDT)的教学模式,对照组采用传统授课式教学(lecture based learing,LBL),通过两组学员的考试成绩和满意度问卷调查比较两种教学方法的教学效果。结果研究组学员的病例分析成绩和总成绩分别为(19.86±2.27)分和(76.59±5.49)分,对照组分别为(18.39±2.42)分和(71.14±7.05)分,研究组和对照组学员对于培养临床思维的满意度分别为95.45%(21/22)和64.29%(18/28),上述指标的组间比较,差异均有统计学意义(t=2.188,P=0.034;t=2.981,P=0.005;χ~2=6.975,P=0.031)。结论 CBL联合MDT的教学模式有利于提高教学质量和培养学生的临床思维。展开更多
目的:探讨DR、CT、MRI三种影像学检查方法在原发性良、恶性骨肿瘤中的诊断价值。材料与方法:按照纳入标准,回顾性收集2012年10月至2013年10月均行X线、CT、MRI检查的骨肿瘤患者33例,每例患者均行手术和病理检查。两名放射科诊断医师分别...目的:探讨DR、CT、MRI三种影像学检查方法在原发性良、恶性骨肿瘤中的诊断价值。材料与方法:按照纳入标准,回顾性收集2012年10月至2013年10月均行X线、CT、MRI检查的骨肿瘤患者33例,每例患者均行手术和病理检查。两名放射科诊断医师分别对DR、CT、MRI诊断原发性骨肿瘤的价值进行评价,骨肿瘤分为良、恶性两组,与病理结果对照,统计其诊断符合率,采用统计学方法比较三种不同检查方法在良、恶性肿瘤的诊断价值。结果:33例骨肿瘤中有14例为恶性骨肿瘤,19例为良性骨肿瘤,DR、CT、MRI三种影像检查方法对原发性良性骨肿瘤的诊断价值有统计学意义(P<0.05),其中MRI和CT在良性骨肿瘤的诊断符合率均高于DR(16/19 vs 8/19,15/19 vs 8/19,P<0.05),MRI和CT的诊断符合率没有统计学意义(16/19 vs 15/19,P>0.05);DR、CT、MRI三种影像检查方法对原发性恶性骨肿瘤的诊断价值没有统计学意义(P>0.05),其诊断符合率分别为50%(7/14),57.1%(8/14),78.6%(11/14)。结论:DR、CT、MRI三种影像学检查方法在诊断原发性良、恶性骨肿瘤中的价值有差别,MRI和CT检查对良性骨肿瘤的诊断价值优于DR。展开更多
基金ThisstudywassupportedinpartbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 0 2 713 14 )
文摘BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.
文摘目的探讨在骨肿瘤疾病临床教学中应用多学科综合诊疗(multidiciplinary treatment,MDT)模式下PBL教学法的教学效果。方法选取新疆医科大学五年制医学影像专业在新疆医科大学附属肿瘤医院进行骨肿瘤疾病临床示教学生共75名,按班级分为实验组(MDT模式下PBL组)和对照组(传统教学LBL组),分别按照各组教学方式进行临床示教,比较2组教学效果和学生满意度的差别。结果 (1)MDT模式下PBL组理论成绩与对照组相比,差异无统计学意义(43.13±4.12 vs 42.76±3.05,P>0.05),但临床思维成绩实验组高于对照组,差异有统计学意义(40.28±3.52 vs 36.13±2.88,P<0.05);(2)在活跃课堂气氛、提高自主学习能力、提高语言表达能力、提高沟通交往能力、提升自信心方面,实验组的满意度高于对照组,差异有统计学意义(P<0.05)。结论 MDT模式下PBL教学法在骨肿瘤疾病临床教学中效果良好,学生满意度较高,符合现代医学教育理念,可以在骨肿瘤临床教学中推广。
文摘目的探讨案例教学法联合多学科协作的教学模式在骨肿瘤临床教学中的应用效果。方法选取2015年1月至2016年12月在我院接受住院医师规范化培训的50名学员作为教学对象,根据所在的病区不同分为研究组(22例)和对照组(28例),研究组采用案例教学(case based learning,CBL)联合多学科协作(multidisciplinary teamwork,MDT)的教学模式,对照组采用传统授课式教学(lecture based learing,LBL),通过两组学员的考试成绩和满意度问卷调查比较两种教学方法的教学效果。结果研究组学员的病例分析成绩和总成绩分别为(19.86±2.27)分和(76.59±5.49)分,对照组分别为(18.39±2.42)分和(71.14±7.05)分,研究组和对照组学员对于培养临床思维的满意度分别为95.45%(21/22)和64.29%(18/28),上述指标的组间比较,差异均有统计学意义(t=2.188,P=0.034;t=2.981,P=0.005;χ~2=6.975,P=0.031)。结论 CBL联合MDT的教学模式有利于提高教学质量和培养学生的临床思维。
文摘目的:探讨DR、CT、MRI三种影像学检查方法在原发性良、恶性骨肿瘤中的诊断价值。材料与方法:按照纳入标准,回顾性收集2012年10月至2013年10月均行X线、CT、MRI检查的骨肿瘤患者33例,每例患者均行手术和病理检查。两名放射科诊断医师分别对DR、CT、MRI诊断原发性骨肿瘤的价值进行评价,骨肿瘤分为良、恶性两组,与病理结果对照,统计其诊断符合率,采用统计学方法比较三种不同检查方法在良、恶性肿瘤的诊断价值。结果:33例骨肿瘤中有14例为恶性骨肿瘤,19例为良性骨肿瘤,DR、CT、MRI三种影像检查方法对原发性良性骨肿瘤的诊断价值有统计学意义(P<0.05),其中MRI和CT在良性骨肿瘤的诊断符合率均高于DR(16/19 vs 8/19,15/19 vs 8/19,P<0.05),MRI和CT的诊断符合率没有统计学意义(16/19 vs 15/19,P>0.05);DR、CT、MRI三种影像检查方法对原发性恶性骨肿瘤的诊断价值没有统计学意义(P>0.05),其诊断符合率分别为50%(7/14),57.1%(8/14),78.6%(11/14)。结论:DR、CT、MRI三种影像学检查方法在诊断原发性良、恶性骨肿瘤中的价值有差别,MRI和CT检查对良性骨肿瘤的诊断价值优于DR。
文摘目的了解恶性骨肿瘤患者希望水平、人格特征及生活质量现状,并探讨患者希望水平和人格特征与生活质量的相关性。方法应用Herth希望量表(Herth hope index,HHI)、艾森克人格问卷简式量表(Eysenck personality questionnaire,EPQ)及生活质量核心问卷(quality of life questionnaire-core 30,QLQ-C30)对160例恶性骨肿瘤患者进行调查。恶性骨肿瘤患者希望水平和人格特征与生活质量的影响采用Pearson相关分析。结果本组恶性骨肿瘤患者整体生活质量量表得分为(57.05±20.09)分,HHI总分(32.88±4.05)分,EPQ量表中的内外向、神经质、精神病质、掩饰倾向得分分别为(54.05±12.29)分、(50.97±11.07)分、(49.14±20.07)分、(52.75±9.01)分。患者希望水平与生活质量呈正相关(P<0.05)。患者人格的内外向和掩饰倾向与其生活质量均呈正相关;患者人格的神经质和精神病质与其生活质量均呈负相关(P<0.05)。结论恶性骨肿瘤患者生活质量较差,护士应根据患者的希望水平及人格特征采取针对性干预策略,以提高其生活质量和心身健康状况。
