瓜类细菌性果斑病(Bacterial fruit blotch of melons,BFB)是由西瓜噬酸菌(Acidovorax citrulli)引起的严重危害西瓜、甜瓜等葫芦科作物的检疫性种传病害。目前,尚未发现免疫或高抗的西瓜、甜瓜商用品种,也缺乏针对性防治药剂,如何高效...瓜类细菌性果斑病(Bacterial fruit blotch of melons,BFB)是由西瓜噬酸菌(Acidovorax citrulli)引起的严重危害西瓜、甜瓜等葫芦科作物的检疫性种传病害。目前,尚未发现免疫或高抗的西瓜、甜瓜商用品种,也缺乏针对性防治药剂,如何高效防治该病害是亟需攻克的生产难题。为了实现瓜类细菌性果斑病的高效防治,科研工作者对果斑病展开了一系列研究并取得较大进展。笔者主要就近5年我国对瓜类细菌性果斑病在病原鉴定、检测方法、致病机制及防治技术等方面的研究进展进行综述,以便于研究者全面了解果斑病的研究现状,并为今后果斑病的研究和防治提供参考。展开更多
Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide.The pathogen is seedtransmitted,so seed detection to prevent distribution of contaminated seed is crucial in dise...Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide.The pathogen is seedtransmitted,so seed detection to prevent distribution of contaminated seed is crucial in disease management.In this study,we adapted a quantitative real-time PCR(qPCR)assay to droplet digital PCR(ddPCR)format for A.citrulli detection by optimizing reaction conditions.The performance of ddPCR in detecting A.citrulli pure culture,DNA,infested watermelon/melon seed and commercial seed samples were compared with multiplex PCR,qPCR,and dilution plating method.The lowest concentrations detected(LCD)by ddPCR reached up to 2 fg DNA,and 102 CFU mL–1 bacterial cells,which were ten times more sensitive than those of the qPCR.When testing artificially infested watermelon and melon seed,0.1%infestation level was detectable using ddPCR and dilution plating method.The 26 positive samples were identified in 201 commercial seed samples through ddPCR,which was the highest positive number among all the methods.High detection sensitivity achieved by ddPCR demonstrated a promising technique for improving seed-transmitted pathogen detection threshold in the future.展开更多
文摘瓜类细菌性果斑病(Bacterial fruit blotch of melons,BFB)是由西瓜噬酸菌(Acidovorax citrulli)引起的严重危害西瓜、甜瓜等葫芦科作物的检疫性种传病害。目前,尚未发现免疫或高抗的西瓜、甜瓜商用品种,也缺乏针对性防治药剂,如何高效防治该病害是亟需攻克的生产难题。为了实现瓜类细菌性果斑病的高效防治,科研工作者对果斑病展开了一系列研究并取得较大进展。笔者主要就近5年我国对瓜类细菌性果斑病在病原鉴定、检测方法、致病机制及防治技术等方面的研究进展进行综述,以便于研究者全面了解果斑病的研究现状,并为今后果斑病的研究和防治提供参考。
基金supported by the the National Key Research and Development Program of China (2017YFD0201602)the National Natural Science Foundation of China (31401704)the Beijing Academy of Agriculture and Forestry Foundation, China (KJCX20180203)
文摘Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide.The pathogen is seedtransmitted,so seed detection to prevent distribution of contaminated seed is crucial in disease management.In this study,we adapted a quantitative real-time PCR(qPCR)assay to droplet digital PCR(ddPCR)format for A.citrulli detection by optimizing reaction conditions.The performance of ddPCR in detecting A.citrulli pure culture,DNA,infested watermelon/melon seed and commercial seed samples were compared with multiplex PCR,qPCR,and dilution plating method.The lowest concentrations detected(LCD)by ddPCR reached up to 2 fg DNA,and 102 CFU mL–1 bacterial cells,which were ten times more sensitive than those of the qPCR.When testing artificially infested watermelon and melon seed,0.1%infestation level was detectable using ddPCR and dilution plating method.The 26 positive samples were identified in 201 commercial seed samples through ddPCR,which was the highest positive number among all the methods.High detection sensitivity achieved by ddPCR demonstrated a promising technique for improving seed-transmitted pathogen detection threshold in the future.
