Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the ba...Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.展开更多
油梨(Persea americana Mill.)又名鳄梨、牛油果和樟梨等,为樟科、鳄梨属,原产中美洲,属热带、亚热带名贵果树之一[1],具有营养价值高、风味好,其消费遍及全球,经济潜力巨大[2]。目前关于国内外病害的报道,主要有根腐病、炭疽病[3]、蒂...油梨(Persea americana Mill.)又名鳄梨、牛油果和樟梨等,为樟科、鳄梨属,原产中美洲,属热带、亚热带名贵果树之一[1],具有营养价值高、风味好,其消费遍及全球,经济潜力巨大[2]。目前关于国内外病害的报道,主要有根腐病、炭疽病[3]、蒂腐病[4]和褐斑病[5]等。2017年5月,作者在海南省儋州市附近的苗圃中发现一种为害油梨苗茎基部的病害,发病率在15%~20%,严重时导致整株植物死亡。展开更多
基金Supported by Science and Technology Research Project of Chongqing Municipal Education Commission(KJ111307)
文摘Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.
文摘油梨(Persea americana Mill.)又名鳄梨、牛油果和樟梨等,为樟科、鳄梨属,原产中美洲,属热带、亚热带名贵果树之一[1],具有营养价值高、风味好,其消费遍及全球,经济潜力巨大[2]。目前关于国内外病害的报道,主要有根腐病、炭疽病[3]、蒂腐病[4]和褐斑病[5]等。2017年5月,作者在海南省儋州市附近的苗圃中发现一种为害油梨苗茎基部的病害,发病率在15%~20%,严重时导致整株植物死亡。
