Objective The aim of this study was to investigate if target-controlled-infusion of remifentanil as sole agent affect bispectal index(BIS) and auditory evoked potential index (AEPI).Methods Twenty two ASAⅠ-Ⅱpatients...Objective The aim of this study was to investigate if target-controlled-infusion of remifentanil as sole agent affect bispectal index(BIS) and auditory evoked potential index (AEPI).Methods Twenty two ASAⅠ-Ⅱpatients scheduled for elective surgery were enrolled in this study.A effect-site target-controlled infusion of remifentanil was started to increase concentration gradually , the initial remifentanil concentration was set at 2.0 ng·mL -1 and was increased by 1.0 ng·mL -1 until 8.0 ng·mL -1.At baseline and at each successive target concentration of remifentanil ,the BIS,AEPI ,observer’s assessment of alertness/sedation (OAA/S) ,hemodynamic variables and respiratory rate were recorded.Results Increasing predicted remifentanil effect-site concentration(CeREMI) decreased BIS value,compared with 0 ng·mL -1,the mean values of BIS were significantly reduced from 4.0 ng·mL -1(P<0.05).No significant difference in AEPI values were found.There was great variation existing in reaction to remifentanil in patients.BIS value decreased obviously in 9 patients(≤70)but in another 10 patients remained unchanged(≥90).In 9 remifentanil-sensitive patients,the spearman correlation coefficient of BIS mean value between CeREMI was -0.715 and between OAA/S was 0.705.However,in another10 remifentanil-insensitive patients,we had not found any correlation in BIS mean value between CeREMI or OAA/S. Conclusion We conclude that at the concentration used in clinical practice,great variation exists in patients’ reaction to remifentanil.Whether remifentanil affects BIS or not depend on if it produce sedative effect in patients.We have to consider the individual difference of BIS in remifentanil application.展开更多
Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to i...Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to identify their structures. Results Sevencompounds were isolated and their structures were identified as ferulic acid (1), conife-rylferukte(2) , bis (2-ethylhexyl) phthalate (3), dibutyl phthalate (4), lignoceric acid (5), palmitic acid(6), and Z-6, 7-cis-dihydroxyligustilide (7) Conclusion Bis (2-ethylhexyl) phthalate and dibutylphthalate were obtained from Angelica sinensis for the first time.展开更多
Trail, a tumor necrosis factor-related apoptosis-inducing ligand, is a novel potent endogenous activator of the cell death pathway through the activation of cell surface death receptors Trail-R1 and Trail-R2. Its role...Trail, a tumor necrosis factor-related apoptosis-inducing ligand, is a novel potent endogenous activator of the cell death pathway through the activation of cell surface death receptors Trail-R1 and Trail-R2. Its role, like FasL in activation-induced cell death (AICD), has been demonstrated in immune system. However the mechanism of Trail induced apoptosis remains unclear. In this report, the recombinant Trail protein was expressed and purified. The apoptosis-inducing activity and the regulation mechanism of recombinant Trail on Jurkat T cells were explored in vitro. Trypan blue exclusion assay demonstrated that the recombinant Trail protein actively killed Jurkat T cells in a dose-dependent manner. Trail-induced apoptosis in Jurkat T cells were remarkably reduced by Bcl-2 over expression in Bcl-2 gene transfected cells. Treatment with PMA (phorbol 12-myristate 13-acetate), a PKC activator, suppressed Trail-induced apoptosis in Jurkat T cells. The inhibition of apoptosis by PMA was abolished by pretreatment with Bis, a PKC inhibitor. Taken together, it was suggested that Bcl-2 over-expression and PMA activated PKC actively down-regulated the Trail-mediated apoptosis in Jurkat T cell.展开更多
文摘Objective The aim of this study was to investigate if target-controlled-infusion of remifentanil as sole agent affect bispectal index(BIS) and auditory evoked potential index (AEPI).Methods Twenty two ASAⅠ-Ⅱpatients scheduled for elective surgery were enrolled in this study.A effect-site target-controlled infusion of remifentanil was started to increase concentration gradually , the initial remifentanil concentration was set at 2.0 ng·mL -1 and was increased by 1.0 ng·mL -1 until 8.0 ng·mL -1.At baseline and at each successive target concentration of remifentanil ,the BIS,AEPI ,observer’s assessment of alertness/sedation (OAA/S) ,hemodynamic variables and respiratory rate were recorded.Results Increasing predicted remifentanil effect-site concentration(CeREMI) decreased BIS value,compared with 0 ng·mL -1,the mean values of BIS were significantly reduced from 4.0 ng·mL -1(P<0.05).No significant difference in AEPI values were found.There was great variation existing in reaction to remifentanil in patients.BIS value decreased obviously in 9 patients(≤70)but in another 10 patients remained unchanged(≥90).In 9 remifentanil-sensitive patients,the spearman correlation coefficient of BIS mean value between CeREMI was -0.715 and between OAA/S was 0.705.However,in another10 remifentanil-insensitive patients,we had not found any correlation in BIS mean value between CeREMI or OAA/S. Conclusion We conclude that at the concentration used in clinical practice,great variation exists in patients’ reaction to remifentanil.Whether remifentanil affects BIS or not depend on if it produce sedative effect in patients.We have to consider the individual difference of BIS in remifentanil application.
文摘Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to identify their structures. Results Sevencompounds were isolated and their structures were identified as ferulic acid (1), conife-rylferukte(2) , bis (2-ethylhexyl) phthalate (3), dibutyl phthalate (4), lignoceric acid (5), palmitic acid(6), and Z-6, 7-cis-dihydroxyligustilide (7) Conclusion Bis (2-ethylhexyl) phthalate and dibutylphthalate were obtained from Angelica sinensis for the first time.
基金Major State BasicResearch (973) Program of China, (G1999053905).
文摘Trail, a tumor necrosis factor-related apoptosis-inducing ligand, is a novel potent endogenous activator of the cell death pathway through the activation of cell surface death receptors Trail-R1 and Trail-R2. Its role, like FasL in activation-induced cell death (AICD), has been demonstrated in immune system. However the mechanism of Trail induced apoptosis remains unclear. In this report, the recombinant Trail protein was expressed and purified. The apoptosis-inducing activity and the regulation mechanism of recombinant Trail on Jurkat T cells were explored in vitro. Trypan blue exclusion assay demonstrated that the recombinant Trail protein actively killed Jurkat T cells in a dose-dependent manner. Trail-induced apoptosis in Jurkat T cells were remarkably reduced by Bcl-2 over expression in Bcl-2 gene transfected cells. Treatment with PMA (phorbol 12-myristate 13-acetate), a PKC activator, suppressed Trail-induced apoptosis in Jurkat T cells. The inhibition of apoptosis by PMA was abolished by pretreatment with Bis, a PKC inhibitor. Taken together, it was suggested that Bcl-2 over-expression and PMA activated PKC actively down-regulated the Trail-mediated apoptosis in Jurkat T cell.
