Molecular imprinting has become a powerful method for the preparation of robust materials that have the ability to recognize a specific chemical species. The stability,ease of preparation and low cost of these materia...Molecular imprinting has become a powerful method for the preparation of robust materials that have the ability to recognize a specific chemical species. The stability,ease of preparation and low cost of these materials have led to their assessment as substitutes for antibodies or enzymes in chemical sensors,catalysis and separations. The present techniques used to prepare molecular imprinting materials most often result in materials exhibiting a high affinity and selectivity but low capacity and poor site accessibility of the target molecules. In this study,we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the AAO template-synthesized sol-gel nanotubes for biochemical separation of estrone. Using of nanotubes as the imprinted materials has the advantage of high affinity,selectivity,capacity and site accessibility for the target molecules. And it is important to emphasize that template route can be used to prepare imprinted nanotubes of nearly any materials. [WT5HZ]展开更多
Cell is the most basic unit of the morphological structure and life activity of an organism. Learning the composition, structure and function of cells, exploring the life activities of cells and studying the interacti...Cell is the most basic unit of the morphological structure and life activity of an organism. Learning the composition, structure and function of cells, exploring the life activities of cells and studying the interaction between cells are of great significance for human cognition and control of the life activities of organisms. Therefore, rapid, convenient, inexpensive, high-precision and reliable methods of cell separation and analysis are being developed to obtain accurate information for the study of cytology and pathology.Microfluidic chip is a new emerging technology in recent years. It has a micromanufacturing structure,which can not only realize the precise space-time control of fluid and cells, but also reproduces the threedimensional dynamic microenvironment of cell growth in the body. In addition, the microfluidic chip has unique microphysical properties and facilitates the integration of microdevices, which provides the possibility of real-time monitoring, continuous culture, separation and enrichment, and even in situ analysis of cells. In this review, we summarized recent advances in the development of different techniques for cell isolation and analysis on microfluidic platforms. Focus was put on biochemical and physical methods for cell separation on microfluidic chips. Subsequent cell analysis depending on fluorescence, Raman, cytomicroscopic imaging, mass spectrometry and electrochemical methods also was remarked. Through analyzing and learning the advantages and disadvantages of different technologies, we hope that microfluidic chips will continue to be improved and expanded for medical and clinical applications.展开更多
The centralized treatment method is a widely used form of wastewater treatment that tends to be less effective at removing toxic substances. Therefore, a detailed analysis of the composition of wastewater can provide ...The centralized treatment method is a widely used form of wastewater treatment that tends to be less effective at removing toxic substances. Therefore, a detailed analysis of the composition of wastewater can provide important information for the design of an effective wastewater treatment process. The objective of this paper was to investigate particle size distribution(PSD), biodegradability, and the chemical composition of the petrochemical wastewater discharges. For this purpose, this project selected the petrochemical wastewater and treated wastewater of China National Offshore Oil Corporation Zhongjie Petrochemical Co, Ltd. as the analysis objects.The step-by-step filtration method, along with a molecular weight classification method, was selected to build the chemical oxygen demand(COD) and biochemical oxygen demand(BOD) fingerprints of petrochemical wastewater and treated wastewater. The results showed that the main pollutants were settleable particles in petrochemical wastewater, which contributed to over 54.85% of the total COD. The colloidal particles with particle sizes in the range of 450–1000 nm had the highest COD value in the treated wastewater, which contributed34.17% of the total COD of treated wastewater. The results of the BOD analysis showed that the soluble fractions were the main reason that treated wastewaters did not meet the treatment standards. Tests on the organic compounds in petrochemical wastewater found that there were mainly linear paraffins, branched paraffins, benzene series compounds, and some plasticizers in the influent of the petrochemical wastewater. The most abundant pollutants in treated petrochemical wastewater were the adjacent diisobutyl phthalate and the linear alkanes.Fourier transform infrared(FTIR) transmission spectroscopy analysis showed that the settleable particles of petrochemical wastewater and membrane bioreactor(MBR)-treated wastewater contained multiple types of organic substances. The results also indicated that removing the oil-settleable substances, t展开更多
文摘Molecular imprinting has become a powerful method for the preparation of robust materials that have the ability to recognize a specific chemical species. The stability,ease of preparation and low cost of these materials have led to their assessment as substitutes for antibodies or enzymes in chemical sensors,catalysis and separations. The present techniques used to prepare molecular imprinting materials most often result in materials exhibiting a high affinity and selectivity but low capacity and poor site accessibility of the target molecules. In this study,we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the AAO template-synthesized sol-gel nanotubes for biochemical separation of estrone. Using of nanotubes as the imprinted materials has the advantage of high affinity,selectivity,capacity and site accessibility for the target molecules. And it is important to emphasize that template route can be used to prepare imprinted nanotubes of nearly any materials. [WT5HZ]
基金supported by the National Natural Science Foundation of China (No. 21874120)the Fundamental Research Funds for the Central Universities (No. 2652019112, 2652018004)the open fund of Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE,Qingdao University of Science and Technology (No. OESACLS202004)。
文摘Cell is the most basic unit of the morphological structure and life activity of an organism. Learning the composition, structure and function of cells, exploring the life activities of cells and studying the interaction between cells are of great significance for human cognition and control of the life activities of organisms. Therefore, rapid, convenient, inexpensive, high-precision and reliable methods of cell separation and analysis are being developed to obtain accurate information for the study of cytology and pathology.Microfluidic chip is a new emerging technology in recent years. It has a micromanufacturing structure,which can not only realize the precise space-time control of fluid and cells, but also reproduces the threedimensional dynamic microenvironment of cell growth in the body. In addition, the microfluidic chip has unique microphysical properties and facilitates the integration of microdevices, which provides the possibility of real-time monitoring, continuous culture, separation and enrichment, and even in situ analysis of cells. In this review, we summarized recent advances in the development of different techniques for cell isolation and analysis on microfluidic platforms. Focus was put on biochemical and physical methods for cell separation on microfluidic chips. Subsequent cell analysis depending on fluorescence, Raman, cytomicroscopic imaging, mass spectrometry and electrochemical methods also was remarked. Through analyzing and learning the advantages and disadvantages of different technologies, we hope that microfluidic chips will continue to be improved and expanded for medical and clinical applications.
基金Supported by the National Basic Research Program of China(2014CB745100)the National Natural Science Foundation of China(21576197)the Tianjin Key Research&Development Program(16YFXTSF00460)
文摘The centralized treatment method is a widely used form of wastewater treatment that tends to be less effective at removing toxic substances. Therefore, a detailed analysis of the composition of wastewater can provide important information for the design of an effective wastewater treatment process. The objective of this paper was to investigate particle size distribution(PSD), biodegradability, and the chemical composition of the petrochemical wastewater discharges. For this purpose, this project selected the petrochemical wastewater and treated wastewater of China National Offshore Oil Corporation Zhongjie Petrochemical Co, Ltd. as the analysis objects.The step-by-step filtration method, along with a molecular weight classification method, was selected to build the chemical oxygen demand(COD) and biochemical oxygen demand(BOD) fingerprints of petrochemical wastewater and treated wastewater. The results showed that the main pollutants were settleable particles in petrochemical wastewater, which contributed to over 54.85% of the total COD. The colloidal particles with particle sizes in the range of 450–1000 nm had the highest COD value in the treated wastewater, which contributed34.17% of the total COD of treated wastewater. The results of the BOD analysis showed that the soluble fractions were the main reason that treated wastewaters did not meet the treatment standards. Tests on the organic compounds in petrochemical wastewater found that there were mainly linear paraffins, branched paraffins, benzene series compounds, and some plasticizers in the influent of the petrochemical wastewater. The most abundant pollutants in treated petrochemical wastewater were the adjacent diisobutyl phthalate and the linear alkanes.Fourier transform infrared(FTIR) transmission spectroscopy analysis showed that the settleable particles of petrochemical wastewater and membrane bioreactor(MBR)-treated wastewater contained multiple types of organic substances. The results also indicated that removing the oil-settleable substances, t
