Exploring the relationship between different structure of the spinal cord and functional assessment after spinal cord injury is important. Quantitative diffusion tensor imaging can provide information about the micros...Exploring the relationship between different structure of the spinal cord and functional assessment after spinal cord injury is important. Quantitative diffusion tensor imaging can provide information about the microstructure of nerve tissue and can quantify the pathological damage of spinal cord white matter and gray matter. In this study, a custom-designed spinal cord contusion-impactor was used to damage the T_（10） spinal cord of beagles. Diffusion tensor imaging was used to observe changes in the whole spinal cord, white matter, and gray matter, and the Texas Spinal Cord Injury Score was used to assess changes in neurological function at 3 hours, 24 hours, 6 weeks, and 12 weeks after injury. With time, fractional anisotropy values after spinal cord injury showed a downward trend, and the apparent diffusion coefficient, mean diffusivity, and radial diffusivity first decreased and then increased. The apparent diffusion-coefficient value was highly associated with the Texas Spinal Cord Injury Score for the whole spinal cord（R = 0.919, P = 0.027）, white matter（R = 0.932, P = 0.021）, and gray matter（R = 0.882, P = 0.048）. Additionally, the other parameters had almost no correlation with the score（P 〉 0.05）. In conclusion, the highest and most significant correlation between diffusion parameters and neurological function was the apparent diffusion-coefficient value for white matter, indicating that it could be used to predict the recovery of neurological function accurately after spinal cord injury.展开更多
目的观察法舒地尔能否通过抑制比格犬支架置入后颈动脉中组织蛋白酶S(Cat S)表达从而抑制再狭窄进程。方法雄性比格犬12只,分为A组(假手术组),B组(裸支架组),C组(裸支架+fasudil组)。将8枚金属裸支架分别置入B组和C组的颈总动脉最狭窄...目的观察法舒地尔能否通过抑制比格犬支架置入后颈动脉中组织蛋白酶S(Cat S)表达从而抑制再狭窄进程。方法雄性比格犬12只,分为A组(假手术组),B组(裸支架组),C组(裸支架+fasudil组)。将8枚金属裸支架分别置入B组和C组的颈总动脉最狭窄处。支架置入2 h后对C组静脉输注法舒地尔进行干预,2 m后行DSA和颈部血管彩超检查,观察颈动脉再狭窄情况;取手术侧血管,进行观察及检测。结果 (1)彩超和DSA结果显示:支架置入后7 d和2 m后比格犬管腔内无血栓形成;支架置入2 m后A组管腔光滑,无狭窄形成;B组狭窄率显著大于C组(P<0.05)。(2)HE染色及弹力纤维染色结果显示:A组颈动脉无内膜增生,中膜由环形排列的弹力纤维和平滑肌细胞组成;B、C组颈动脉中膜平滑肌细胞层排列稍紊乱,内膜增生明显;B组新生内膜厚度、面积、狭窄率均显著大于C组(P<0.05)。(3)Cat S mRNA RT-PCR结果显示:A组比格犬颈动脉有少量Cat S mRNA表达;与A组比较,B、C组Cat S mRNA表达显著升高(P<0.05);B组Cat S mRNA表达显著高于C组(P<0.05)。(4)α-SMA免疫组化结果显示:A、B、C组中膜上均有大量α-SMA阳性表达。A组内弹力膜外无α-SMA阳性表达,B、C组新生内膜上见α-SMA阳性细胞。(5)PCNA免疫组化结果显示:A组PCNA免疫组化阳性染色较少,B、C组在支架周围及新生内膜上PCNA阳性表达增多,B组新生内膜上PCNA平均光密度值显著高于C组(P<0.05)。结论法舒地尔能通过抑制比格犬支架置入后颈动脉中组织蛋白酶S的表达,从而抑制再狭窄的进展。展开更多
BACKGROUND The magnetic compression technique has been used to establish an animal model of tracheoesophageal fistula(TEF),but the commonly shaped magnets present limitations of poor homogeneity of TEF and poor model ...BACKGROUND The magnetic compression technique has been used to establish an animal model of tracheoesophageal fistula(TEF),but the commonly shaped magnets present limitations of poor homogeneity of TEF and poor model control.We designed a Tshaped magnet system to overcome these problems and verified its effectiveness via animal experiments.AIM To investigate the effectiveness of a T-shaped magnet system for establishing a TEF model in beagle dogs.METHODS Twelve beagles were randomly assigned to groups in which magnets of the Tshaped scheme(study group,n=6)or normal magnets(control group,n=6)were implanted into the trachea and esophagus separately under gastroscopy.Operation time,operation success rate,and accidental injury were recorded.After operation,the presence and timing of cough and the time of magnet shedding were observed.Dogs in the control group were euthanized after X-ray and gastroscopy to confirm establishment of TEFs after coughing,and gross specimens of TEFs were obtained.Dogs in the study group were euthanized after X-ray and gastroscopy 2 wk after surgery,and gross specimens were obtained.Fistula size was measured in all animals,and then harvested fistula specimens were examined by hematoxylin and eosin(HE)and Masson trichrome staining.RESULTS The operation success rate was 100%for both groups.Operation time did not differ between the study group(5.25 min±1.29 min)and the control group(4.75 min±1.70 min;P=0.331).No bleeding,perforation,or unplanned magnet attraction occurred in any animal during the operation.In the early postoperative period,all dogs ate freely and were generally in good condition.Dogs in the control group had severe cough after drinking water at 6-9 d after surgery.X-ray indicated that the magnets had entered the stomach,and gastroscopy showed TEF formation.Gross specimens of TEFs from the control group showed the formation of fistulas with a diameter of 4.94 mm±1.29 mm(range,3.52-6.56 mm).HE and Masson trichrome staining showed scar tissue formation and hierarchical structu展开更多
Background: The aims of this study were(a) to ascertain age-related changes in the reference values in hematological and serum biochemical examinations of beagles,and(b) to clarify the changes in these findings, inclu...Background: The aims of this study were(a) to ascertain age-related changes in the reference values in hematological and serum biochemical examinations of beagles,and(b) to clarify the changes in these findings, including acute phase proteins and oxidative stress, throughout pregnancy and after parturition.Methods: Clinicopathological parameters were measured in young beagles at 6, 9 and 12 months and in adult beagles aged from 24 to 60 months. Likewise, pregnant beagles were investigated throughout the pregnancy and after parturition.Results: Apparent age-related changes were found in erythrocytic parameters during the growth and development of beagles. Most of the parameters(total protein,albumin, blood urea nitrogen, creatinine, urate, alkaline phosphatase(ALP) and creatine kinase(CK) exhibited age-dependent transitions. White cell count significantly increased after 30 days of pregnancy. The values of erythrocytic parameters moderately decreased during the second half of the pregnancy. Triglycerides, total cholesterol, free cholesterol and phospholipid concentrations increased in the mid- and late stages of pregnancy. ALP, lactate dehydrogenase, CK and cholinesterase activities markedly increased during pregnancy and/or after parturition. C-reactive protein(CRP) concentrations gradually increased and reached a maximum after 30-40 days of pregnancy. Serum amyloid A(SAA) levels markedly increased at 30 days of pregnancy before subsiding, and then increased again 3 days after parturition. Reactive oxygen metabolites(d-ROMs) showed significant increases after 30 and 40 days of pregnancy.Conclusions: Reference values for hematological and serum biochemical examinations should be used for health evaluation of dogs, taking sex, age and the stage of pregnancy into consideration. Measurements of CRP, SAA and d-ROM levels are also useful for assessing maternal conditions in mid-pregnancy.展开更多
BACKGROUND Magnetic compression anastomosis(MCA)is a simple procedure contributing to a reliable anastomosis.However,digestive-tract reconstruction after total gastrectomy using MCA has not yet been reported.AIM To in...BACKGROUND Magnetic compression anastomosis(MCA)is a simple procedure contributing to a reliable anastomosis.However,digestive-tract reconstruction after total gastrectomy using MCA has not yet been reported.AIM To investigate the feasibility of MCA for simultaneous esophagojejunostomy and jejunojejunostomy after total gastrectomy using beagle dogs.METHODS Sixteen beagles were randomly divided into an MCA group(study group,n=8)and a manual-suture anastomosis group(control group,n=8).Two different magnetic anastomosis devices were used in the study group for esophagojejunal and jejunojejunal anastomoses.Both devices included a pair of circular daughter and parent magnets each.The time of esophagojejunostomy and jejunojejunostomy,postoperative complications,and survival rate of the two groups were compared.The dogs were sacrificed one month after the operation and their anastomotic specimens were obtained.Healing was observed by the naked eye and a light microscope.RESULTS Digestive-tract reconstruction after total gastrectomy was successfully completed in both groups(survival rate=100%).In the study group,esophagojejunal and jejunojejunal anastomoses took 6.13±0.58 and 4.06±0.42 min,respectively,significantly lower than those in the control group(15.63±1.53 min,P<0.001 and 10.31±1.07 min,P<0.001,respectively).Complications such as bleeding,anastomotic leakage,and anastomotic stenosis were not observed.In the study group,the magnets did not interfere with each other.Discharge time of the jejunojejunal magnetic anastomosis device was 10.75±1.28 d,while that of the esophagojejunal magnetic anastomosis device was 12.25±1.49 d.Residual silk was found in the control group.The study group showed a greater smoothness of the anastomosis than that of the control group.All layers of anastomosis healed well in both groups.CONCLUSION MCA is a safe and feasible procedure for digestive-tract reconstruction after total gastrectomy in this animal model.展开更多
为检测比格犬α和β-干扰素(CaIFN-α和CaIFN-β),本研究根据GenBank中登录的IFN-α和IFN-β基因序列,分别设计合成特异性引物,以犬3-磷酸甘油脱氢酶(GAPDH)mRNA为内参,建立SYBR Green I荧光定量RT-PCR检测方法。通过RT-PCR分别扩增IFN...为检测比格犬α和β-干扰素(CaIFN-α和CaIFN-β),本研究根据GenBank中登录的IFN-α和IFN-β基因序列,分别设计合成特异性引物,以犬3-磷酸甘油脱氢酶(GAPDH)mRNA为内参,建立SYBR Green I荧光定量RT-PCR检测方法。通过RT-PCR分别扩增IFN-α、IFN-β和GAPDH的基因片段,并克隆于pMD19-T载体中制备标准品,建立荧光定量RT-PCR标准曲线。结果表明:IFN-α和IFN-β和GAPDH基因的Ct值与标准品稀释度在1×102拷贝/μL^1×108拷贝/μL内均呈良好的线性关系,相关系数均大于0.994。组内组间变异系数均小于3%,特异性和重复性较好。同时采用常规RT-PCR方法与本研究建立的方法对30份临床样品检测,结果显示IFN-α和IFN-β的阳性符合率分别为96.43%和96.55%。本研究建立的检测方法为比格犬IFN mRNA的定量分析提供了有效手段。展开更多
基金supported by the National Natural Science Foundation of China,No.81272164the Special Fund for Basic Scientific Research of Central Public Research Institutes in China,No.2015CZ-6,2016CZ-4+2 种基金the Beijing Institute for Brain Disorders in China,No.201601,0000-100031the Supporting Program of the “Twelve Five-year Plan” for Science&Technology Research of China,No.2012BAI34B02a grant from the Ministry of Science and Technology of China,No.2015CB351701
文摘Exploring the relationship between different structure of the spinal cord and functional assessment after spinal cord injury is important. Quantitative diffusion tensor imaging can provide information about the microstructure of nerve tissue and can quantify the pathological damage of spinal cord white matter and gray matter. In this study, a custom-designed spinal cord contusion-impactor was used to damage the T_（10） spinal cord of beagles. Diffusion tensor imaging was used to observe changes in the whole spinal cord, white matter, and gray matter, and the Texas Spinal Cord Injury Score was used to assess changes in neurological function at 3 hours, 24 hours, 6 weeks, and 12 weeks after injury. With time, fractional anisotropy values after spinal cord injury showed a downward trend, and the apparent diffusion coefficient, mean diffusivity, and radial diffusivity first decreased and then increased. The apparent diffusion-coefficient value was highly associated with the Texas Spinal Cord Injury Score for the whole spinal cord（R = 0.919, P = 0.027）, white matter（R = 0.932, P = 0.021）, and gray matter（R = 0.882, P = 0.048）. Additionally, the other parameters had almost no correlation with the score（P 〉 0.05）. In conclusion, the highest and most significant correlation between diffusion parameters and neurological function was the apparent diffusion-coefficient value for white matter, indicating that it could be used to predict the recovery of neurological function accurately after spinal cord injury.
文摘目的观察法舒地尔能否通过抑制比格犬支架置入后颈动脉中组织蛋白酶S(Cat S)表达从而抑制再狭窄进程。方法雄性比格犬12只,分为A组(假手术组),B组(裸支架组),C组(裸支架+fasudil组)。将8枚金属裸支架分别置入B组和C组的颈总动脉最狭窄处。支架置入2 h后对C组静脉输注法舒地尔进行干预,2 m后行DSA和颈部血管彩超检查,观察颈动脉再狭窄情况;取手术侧血管,进行观察及检测。结果 (1)彩超和DSA结果显示:支架置入后7 d和2 m后比格犬管腔内无血栓形成;支架置入2 m后A组管腔光滑,无狭窄形成;B组狭窄率显著大于C组(P<0.05)。(2)HE染色及弹力纤维染色结果显示:A组颈动脉无内膜增生,中膜由环形排列的弹力纤维和平滑肌细胞组成;B、C组颈动脉中膜平滑肌细胞层排列稍紊乱,内膜增生明显;B组新生内膜厚度、面积、狭窄率均显著大于C组(P<0.05)。(3)Cat S mRNA RT-PCR结果显示:A组比格犬颈动脉有少量Cat S mRNA表达;与A组比较,B、C组Cat S mRNA表达显著升高(P<0.05);B组Cat S mRNA表达显著高于C组(P<0.05)。(4)α-SMA免疫组化结果显示:A、B、C组中膜上均有大量α-SMA阳性表达。A组内弹力膜外无α-SMA阳性表达,B、C组新生内膜上见α-SMA阳性细胞。(5)PCNA免疫组化结果显示:A组PCNA免疫组化阳性染色较少,B、C组在支架周围及新生内膜上PCNA阳性表达增多,B组新生内膜上PCNA平均光密度值显著高于C组(P<0.05)。结论法舒地尔能通过抑制比格犬支架置入后颈动脉中组织蛋白酶S的表达,从而抑制再狭窄的进展。
基金Supported by the Key Research&Development Program of Shaanxi Province of China,No.2024SF-YBXM-447Institutional Foundation of The First Affiliated Hospital of Xi’an Jiaotong University,No.2022MS-07+1 种基金Fundamental Research Funds for the Central Universities,No.xzy022023068Natural Science Foundation of Shaanxi Province,No.2023-JC-QN-0814.
文摘BACKGROUND The magnetic compression technique has been used to establish an animal model of tracheoesophageal fistula(TEF),but the commonly shaped magnets present limitations of poor homogeneity of TEF and poor model control.We designed a Tshaped magnet system to overcome these problems and verified its effectiveness via animal experiments.AIM To investigate the effectiveness of a T-shaped magnet system for establishing a TEF model in beagle dogs.METHODS Twelve beagles were randomly assigned to groups in which magnets of the Tshaped scheme(study group,n=6)or normal magnets(control group,n=6)were implanted into the trachea and esophagus separately under gastroscopy.Operation time,operation success rate,and accidental injury were recorded.After operation,the presence and timing of cough and the time of magnet shedding were observed.Dogs in the control group were euthanized after X-ray and gastroscopy to confirm establishment of TEFs after coughing,and gross specimens of TEFs were obtained.Dogs in the study group were euthanized after X-ray and gastroscopy 2 wk after surgery,and gross specimens were obtained.Fistula size was measured in all animals,and then harvested fistula specimens were examined by hematoxylin and eosin(HE)and Masson trichrome staining.RESULTS The operation success rate was 100%for both groups.Operation time did not differ between the study group(5.25 min±1.29 min)and the control group(4.75 min±1.70 min;P=0.331).No bleeding,perforation,or unplanned magnet attraction occurred in any animal during the operation.In the early postoperative period,all dogs ate freely and were generally in good condition.Dogs in the control group had severe cough after drinking water at 6-9 d after surgery.X-ray indicated that the magnets had entered the stomach,and gastroscopy showed TEF formation.Gross specimens of TEFs from the control group showed the formation of fistulas with a diameter of 4.94 mm±1.29 mm(range,3.52-6.56 mm).HE and Masson trichrome staining showed scar tissue formation and hierarchical structu
文摘Background: The aims of this study were(a) to ascertain age-related changes in the reference values in hematological and serum biochemical examinations of beagles,and(b) to clarify the changes in these findings, including acute phase proteins and oxidative stress, throughout pregnancy and after parturition.Methods: Clinicopathological parameters were measured in young beagles at 6, 9 and 12 months and in adult beagles aged from 24 to 60 months. Likewise, pregnant beagles were investigated throughout the pregnancy and after parturition.Results: Apparent age-related changes were found in erythrocytic parameters during the growth and development of beagles. Most of the parameters(total protein,albumin, blood urea nitrogen, creatinine, urate, alkaline phosphatase(ALP) and creatine kinase(CK) exhibited age-dependent transitions. White cell count significantly increased after 30 days of pregnancy. The values of erythrocytic parameters moderately decreased during the second half of the pregnancy. Triglycerides, total cholesterol, free cholesterol and phospholipid concentrations increased in the mid- and late stages of pregnancy. ALP, lactate dehydrogenase, CK and cholinesterase activities markedly increased during pregnancy and/or after parturition. C-reactive protein(CRP) concentrations gradually increased and reached a maximum after 30-40 days of pregnancy. Serum amyloid A(SAA) levels markedly increased at 30 days of pregnancy before subsiding, and then increased again 3 days after parturition. Reactive oxygen metabolites(d-ROMs) showed significant increases after 30 and 40 days of pregnancy.Conclusions: Reference values for hematological and serum biochemical examinations should be used for health evaluation of dogs, taking sex, age and the stage of pregnancy into consideration. Measurements of CRP, SAA and d-ROM levels are also useful for assessing maternal conditions in mid-pregnancy.
基金the Institutional Foundation of The First Affiliated Hospital of Xi’an Jiaotong University,No.2022MS-07(to Yan XP)Key Research and Development Plan of Shaanxi Province,No.2021GXLH-Z-009(to Li Y).
文摘BACKGROUND Magnetic compression anastomosis(MCA)is a simple procedure contributing to a reliable anastomosis.However,digestive-tract reconstruction after total gastrectomy using MCA has not yet been reported.AIM To investigate the feasibility of MCA for simultaneous esophagojejunostomy and jejunojejunostomy after total gastrectomy using beagle dogs.METHODS Sixteen beagles were randomly divided into an MCA group(study group,n=8)and a manual-suture anastomosis group(control group,n=8).Two different magnetic anastomosis devices were used in the study group for esophagojejunal and jejunojejunal anastomoses.Both devices included a pair of circular daughter and parent magnets each.The time of esophagojejunostomy and jejunojejunostomy,postoperative complications,and survival rate of the two groups were compared.The dogs were sacrificed one month after the operation and their anastomotic specimens were obtained.Healing was observed by the naked eye and a light microscope.RESULTS Digestive-tract reconstruction after total gastrectomy was successfully completed in both groups(survival rate=100%).In the study group,esophagojejunal and jejunojejunal anastomoses took 6.13±0.58 and 4.06±0.42 min,respectively,significantly lower than those in the control group(15.63±1.53 min,P<0.001 and 10.31±1.07 min,P<0.001,respectively).Complications such as bleeding,anastomotic leakage,and anastomotic stenosis were not observed.In the study group,the magnets did not interfere with each other.Discharge time of the jejunojejunal magnetic anastomosis device was 10.75±1.28 d,while that of the esophagojejunal magnetic anastomosis device was 12.25±1.49 d.Residual silk was found in the control group.The study group showed a greater smoothness of the anastomosis than that of the control group.All layers of anastomosis healed well in both groups.CONCLUSION MCA is a safe and feasible procedure for digestive-tract reconstruction after total gastrectomy in this animal model.
文摘为检测比格犬α和β-干扰素(CaIFN-α和CaIFN-β),本研究根据GenBank中登录的IFN-α和IFN-β基因序列,分别设计合成特异性引物,以犬3-磷酸甘油脱氢酶(GAPDH)mRNA为内参,建立SYBR Green I荧光定量RT-PCR检测方法。通过RT-PCR分别扩增IFN-α、IFN-β和GAPDH的基因片段,并克隆于pMD19-T载体中制备标准品,建立荧光定量RT-PCR标准曲线。结果表明:IFN-α和IFN-β和GAPDH基因的Ct值与标准品稀释度在1×102拷贝/μL^1×108拷贝/μL内均呈良好的线性关系,相关系数均大于0.994。组内组间变异系数均小于3%,特异性和重复性较好。同时采用常规RT-PCR方法与本研究建立的方法对30份临床样品检测,结果显示IFN-α和IFN-β的阳性符合率分别为96.43%和96.55%。本研究建立的检测方法为比格犬IFN mRNA的定量分析提供了有效手段。
