目的:检测杀菌性/通透性增加蛋白(BPI)和脂多糖结合蛋白(LBP)基因单个核苷酸多态性(SNP)的频率分布。方法:选择该院部分科室ICU病房并发全身性感染的患者112例,并以100名正常健康者为对照组,取外周血白细胞提取基因组DNA,用聚合酶链反应...目的:检测杀菌性/通透性增加蛋白(BPI)和脂多糖结合蛋白(LBP)基因单个核苷酸多态性(SNP)的频率分布。方法:选择该院部分科室ICU病房并发全身性感染的患者112例,并以100名正常健康者为对照组,取外周血白细胞提取基因组DNA,用聚合酶链反应-限制性内切酶长度多态性分析(RFLP-PCR)法检测BPI基因上Lys216G lu、PstⅠin intron 5和G545C、LBP基因上Cys98G ly和Leu436Pro共5个位点SNP。结果:全身性感染组BPI Lys216G lu G/G纯合子及G等位基因出现的频率明显高于对照组;死亡组BPI Lys216G lu G/G纯合子及G等位基因出现的频率也明显高于存活组。结论:BPI Lys216G lu SNP的差异可能是全身性感染患者易感原因之一。展开更多
Bactericidal/permeability-increasing protein （BPI） is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes ...Bactericidal/permeability-increasing protein （BPI） is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes and epididymides as well as the subcellular localization within epididymal spermatozoa. Our results showed that, BPI mRNA was expressed in testis and epididymis independently. Throughout the epididymis, the BPI protein level gradually decreased in the epididymal epithelium in a spatial manner, specialized within the cytoplasm of clear cells in the cauda part. We detected BPI proteins in intact acrosome, implying its testicular origin; on the other hand, after the acrosome reaction, BPI proteins were observed dispersed across the entire sperm head, especially enriched at the equatorial segment. Our findings suggested a dual origin of the BPI that generated both in the testis and epididymis, and associated with mouse spermatozoa. BPI protein might be involved in the dynamics modification of the sperm plasma membrane and also the fertilization process.展开更多
objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. co...objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. coli 026:B6 LPS was injected at a dosage of 12. 5 mg/kg through the artery to reproduce endo toxic shock. BPIP at a dosage of 5 mg/kg (BPIP-treated group) or equal volume of normal saline (control group) were injected immediately after the injection of LPS. Results: ①Survival time of the shocked animals was prolonged and the 24 h survival rate was also significantly increased in BPIP-treated group as compared with the control group. ②The mean arterial pressure, left intraventricular systolic pressure, isovolemic ven tricular pressure and ±dp/dtmax. were significantly higher in BPIP-treated group than in control group. ③ Plasma levels of glutamic-pyruvic transaminase and urea nitrogen were markedly higher but those of endotox in and TNFα were lower in BPIP-treated group than in control group. Conclusion: BPIP can exert significant protective effects on cardiac, hepatic and renal functions in rats after endotoxic shock, indicating that BPIP might be a good choice in treatment of sepsis/septic shock.展开更多
文摘目的:检测杀菌性/通透性增加蛋白(BPI)和脂多糖结合蛋白(LBP)基因单个核苷酸多态性(SNP)的频率分布。方法:选择该院部分科室ICU病房并发全身性感染的患者112例,并以100名正常健康者为对照组,取外周血白细胞提取基因组DNA,用聚合酶链反应-限制性内切酶长度多态性分析(RFLP-PCR)法检测BPI基因上Lys216G lu、PstⅠin intron 5和G545C、LBP基因上Cys98G ly和Leu436Pro共5个位点SNP。结果:全身性感染组BPI Lys216G lu G/G纯合子及G等位基因出现的频率明显高于对照组;死亡组BPI Lys216G lu G/G纯合子及G等位基因出现的频率也明显高于存活组。结论:BPI Lys216G lu SNP的差异可能是全身性感染患者易感原因之一。
文摘Bactericidal/permeability-increasing protein （BPI） is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes and epididymides as well as the subcellular localization within epididymal spermatozoa. Our results showed that, BPI mRNA was expressed in testis and epididymis independently. Throughout the epididymis, the BPI protein level gradually decreased in the epididymal epithelium in a spatial manner, specialized within the cytoplasm of clear cells in the cauda part. We detected BPI proteins in intact acrosome, implying its testicular origin; on the other hand, after the acrosome reaction, BPI proteins were observed dispersed across the entire sperm head, especially enriched at the equatorial segment. Our findings suggested a dual origin of the BPI that generated both in the testis and epididymis, and associated with mouse spermatozoa. BPI protein might be involved in the dynamics modification of the sperm plasma membrane and also the fertilization process.
文摘objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. coli 026:B6 LPS was injected at a dosage of 12. 5 mg/kg through the artery to reproduce endo toxic shock. BPIP at a dosage of 5 mg/kg (BPIP-treated group) or equal volume of normal saline (control group) were injected immediately after the injection of LPS. Results: ①Survival time of the shocked animals was prolonged and the 24 h survival rate was also significantly increased in BPIP-treated group as compared with the control group. ②The mean arterial pressure, left intraventricular systolic pressure, isovolemic ven tricular pressure and ±dp/dtmax. were significantly higher in BPIP-treated group than in control group. ③ Plasma levels of glutamic-pyruvic transaminase and urea nitrogen were markedly higher but those of endotox in and TNFα were lower in BPIP-treated group than in control group. Conclusion: BPIP can exert significant protective effects on cardiac, hepatic and renal functions in rats after endotoxic shock, indicating that BPIP might be a good choice in treatment of sepsis/septic shock.
