The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair ...The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase（ACh E） staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction.展开更多
Empirical mode decomposition (EMD) is an adaptive and approximately orthogonal filtering process that reflects human’s visual mechanism of differentiating textures. In this paper, we present a modified 2D EMD algorit...Empirical mode decomposition (EMD) is an adaptive and approximately orthogonal filtering process that reflects human’s visual mechanism of differentiating textures. In this paper, we present a modified 2D EMD algorithm using the FastRBF and an appropriate number of iterations in the shifting process (SP), then apply it to texture classification. Rotation-invariant texture feature vectors are extracted using auto-registration and circular regions of magnitude spectra of 2D fast Fourier transform (FFT). In the experiments, we employ a Bayesion classifier to classify a set of 15 distinct natural textures selected from the Brodatz album. The experimental results, based on different testing datasets for images with different orientations, show the effectiveness of the proposed classification scheme.展开更多
基金supported by the National Natural Science Foundation of China,No.30571913a grant from the Science and Technology Project of Guangdong Province of China,No.2013B010404019+1 种基金the Natural Science Foundation of Guangdong Province of China,No.9151008901000006the Medical Scientific Research Foundation of Guangdong Province of China,No.A2009173
文摘The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase（ACh E） staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction.
基金Project supported by the National Basic Research Program (973) of China (Nos. 2004CB318000 and 2002CB312104), the National Natural Science Foundation of China (Nos. 60133020 and 60325208) and the Natural Science Foundation of Beijing (No. 1062006), China
文摘Empirical mode decomposition (EMD) is an adaptive and approximately orthogonal filtering process that reflects human’s visual mechanism of differentiating textures. In this paper, we present a modified 2D EMD algorithm using the FastRBF and an appropriate number of iterations in the shifting process (SP), then apply it to texture classification. Rotation-invariant texture feature vectors are extracted using auto-registration and circular regions of magnitude spectra of 2D fast Fourier transform (FFT). In the experiments, we employ a Bayesion classifier to classify a set of 15 distinct natural textures selected from the Brodatz album. The experimental results, based on different testing datasets for images with different orientations, show the effectiveness of the proposed classification scheme.
