In order to explore the transfer mechanism of chromium（Ⅲ） in mammals, a novel complex [Cr（ASA）（en）2]Cl·2H2O, bis（ethylenediamine-κ2 N,N′）（4-aminosalicylic acid-κ^2 O,O′） chromium（Ⅲ） monochloride...In order to explore the transfer mechanism of chromium（Ⅲ） in mammals, a novel complex [Cr（ASA）（en）2]Cl·2H2O, bis（ethylenediamine-κ2 N,N′）（4-aminosalicylic acid-κ^2 O,O′） chromium（Ⅲ） monochloride dihydrate was synthesized （4-aminosalicylic acid=H2ASA, ethylenediamine=en）. The crystal structure belongs to orthorhombic system with the space group P212121 by means of X-ray diffraction. The characteristic for transfer of Cr^3＋ from the compound to the low-molecular-mass chelator EDTA and the iron-binding protein apoovotransferrin （apoOTf） was followed by UV-visible （UV-Vis） and fluorescence spectra in 0.01 mol·L^-1 Hepes at pH 7.4. The second order rate constants were calculated. Those spectra in conjunction were used to obtain more accurate information about the interaction of chromium complex with apoOTf. The experimental results indicate that Cr^3＋ can be transferred from the complex to apoOTf with the retention of the 4-aminosalicylic acid acting as a synergistic anion.展开更多
基金Project supportedby the National Natural Science Foundation of China （Nos. 20371031 and 20771068） and the Natural Science Foundation of Shanxi Province （No. 2007011024）.
文摘In order to explore the transfer mechanism of chromium（Ⅲ） in mammals, a novel complex [Cr（ASA）（en）2]Cl·2H2O, bis（ethylenediamine-κ2 N,N′）（4-aminosalicylic acid-κ^2 O,O′） chromium（Ⅲ） monochloride dihydrate was synthesized （4-aminosalicylic acid=H2ASA, ethylenediamine=en）. The crystal structure belongs to orthorhombic system with the space group P212121 by means of X-ray diffraction. The characteristic for transfer of Cr^3＋ from the compound to the low-molecular-mass chelator EDTA and the iron-binding protein apoovotransferrin （apoOTf） was followed by UV-visible （UV-Vis） and fluorescence spectra in 0.01 mol·L^-1 Hepes at pH 7.4. The second order rate constants were calculated. Those spectra in conjunction were used to obtain more accurate information about the interaction of chromium complex with apoOTf. The experimental results indicate that Cr^3＋ can be transferred from the complex to apoOTf with the retention of the 4-aminosalicylic acid acting as a synergistic anion.
