High levels of plasma high-density lipoproteins (HDL) are associated with a low incidence of cardiovascular disease[1]. HDL contains two major apolipoproteins (apo):apoAI and apoAII. It is generally accepted that apoA...High levels of plasma high-density lipoproteins (HDL) are associated with a low incidence of cardiovascular disease[1]. HDL contains two major apolipoproteins (apo):apoAI and apoAII. It is generally accepted that apoAI plays a central role in reverse cholesterol transport and protects against atherosclerosis[2,3]; however,apoAII functions have not been clearly characterized[4-6].展开更多
文摘High levels of plasma high-density lipoproteins (HDL) are associated with a low incidence of cardiovascular disease[1]. HDL contains two major apolipoproteins (apo):apoAI and apoAII. It is generally accepted that apoAI plays a central role in reverse cholesterol transport and protects against atherosclerosis[2,3]; however,apoAII functions have not been clearly characterized[4-6].
文摘目的 建立中压液相层析分离法纯化人 HDL 主要载脂蛋白 A 、A 等的方法。方法 以人血清高密度脂蛋白制品为原料 ,经一次性密度梯度超离心法纯化 HDL。去脂 HDL 经中压 Sephacryl S- 2 0 0柱层析获apo E、A 、A 及 Cs粗品。用 Sephadex G- 75柱层析分离 apo A 及 A 。结果 纯化的载脂蛋白 apo A 及 A 经免疫和 SDS- PAGE鉴定为免疫纯及电泳纯。结论 建立了一种可大量、快速、高分辨分离纯化载脂蛋白 apo A 及 A 的方法 ,为今后载脂蛋白和脂蛋白的基础和临床研究提供必要的实验材料。
