【目的】探讨采后不同处理方式对杏鲍菇贮藏品质及抗氧化酶系统的影响,为杏鲍菇贮藏保鲜技术的应用与发展提供理论指导。【方法】以新鲜采收的杏鲍菇为试验材料,分别采用0.03 mm的聚乙烯(PE)薄膜袋封口包装、0.05 mm的PE薄膜袋封口包装...【目的】探讨采后不同处理方式对杏鲍菇贮藏品质及抗氧化酶系统的影响,为杏鲍菇贮藏保鲜技术的应用与发展提供理论指导。【方法】以新鲜采收的杏鲍菇为试验材料,分别采用0.03 mm的聚乙烯(PE)薄膜袋封口包装、0.05 mm的PE薄膜袋封口包装和1.5%壳聚糖涂膜3种处理,以0.05 mm PE保鲜袋不封口为对照,于4℃、相对湿度85%—95%的条件下贮藏,通过测定失重率、色差值、硬度、可溶性固形物(TSS)、还原糖及可溶性蛋白质含量等理化指标,以及过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)等3种抗氧化酶活性以及相对电导率、丙二醛(MDA)、超氧阴离子()、过氧化氢(H2O2)的含量,研究不同采后处理方式对杏鲍菇贮藏品质及抗氧化酶系统的影响。【结果】与PE不封口处理相比,两种厚度的PE保鲜袋处理能显著降低杏鲍菇子实体失重率(P<0.01),在贮藏18 d时,两种处理的失重率仅为0.35%和0.20%;两种PE处理的菇体硬度显著高于对照(P<0.05),且在贮藏期间两种处理均分别在第3天和第12天出现了两个硬度峰值;PE处理不易发生褐变,但在贮藏末期,0.03 mm PE处理的褐变程度显著增加(P<0.05)。两种PE处理均能显著抑制贮藏期间TSS和可溶性蛋白的增加,减缓还原糖的消耗。1.5%壳聚糖涂膜处理在0—9 d菇体硬度显著增加(P<0.05),随后硬度快速下降;其失重率较高,与对照无显著差异(P>0.05);贮藏期间褐变指数低,但在贮藏末期的褐变程度显著(P<0.05);贮藏的中期与后期,TSS、还原糖和可溶性蛋白含量的变化均显著低于对照。3种处理在一定程度上提高了杏鲍菇的抗氧化能力,与对照处理相比,3种处理均能显著降低膜透性和脂膜过氧化产物MDA的含量(P<0.05),但在贮藏中、后期,壳聚糖处理的电导率和MDA含量均显著高于两种PE处理。0.03 mm PE和1.5%壳聚糖涂膜处理能显著降低超氧阴离子产生速率和过氧化氢�展开更多
The effect of thermal stress on the antioxidant system was investigated in two invasive plants, Eupatorium adenophorum Spreng. and E. odoratum L. The former is sensitive to high temperature, whereas the latter is sens...The effect of thermal stress on the antioxidant system was investigated in two invasive plants, Eupatorium adenophorum Spreng. and E. odoratum L. The former is sensitive to high temperature, whereas the latter is sensitive to low temperature. Our aim was to explore the relationship between the response of antioxidant enzymes and temperature in the two invasive weeds with different distribution patterns in China. Plants were transferred from glasshouse to growth chambers at a constant 25 ℃for I week to acclimatize to the environment. For the heat treatments, temperature was increased stepwise to 30, 35, 38 and finally to 42 ℃. For the cold treatments, temperature was decreased stepwise to 20, 15, 10 and finally to 5 ℃. Plants were kept in the growth chambers for 24 h at each temperature step. In E. adenophorum, the coordinated increase of the activities of antioxidant enzymes was effective in protecting the plant from the accumulation of active oxygen species （AOS） at low temperature, but the activities of catalase （CAT）, guaiacol peroxidase （POD）, ascorbate peroxidase （APX）, glutathione reductase （GR）, and monodehydroascorbate reductase （MDAR） were not accompanied by the increase of superoxide dismutase （SOD） during the heat treatments. As a result, the level of lipid peroxidation in E. adenophorum was higher under heat stress than under cold stress. In E. odoratum, however, the lesser degree of membrane damage, as indicated by low monodehydroascorbate content, and the coordinated increase of the oxygen. Datoxifying enzymes were observed in heat-treated plants, but the antioxidant enzymes were unable to operate in cold stress. This indicates that the plants have a higher capacity for scavenging oxygen radicals in heat stress than in cold stress. The different responses of antioxidant enzymes may be one of the possible mechanisms of the differences in temperature sensitivities of the two plant species.展开更多
文摘【目的】探讨采后不同处理方式对杏鲍菇贮藏品质及抗氧化酶系统的影响,为杏鲍菇贮藏保鲜技术的应用与发展提供理论指导。【方法】以新鲜采收的杏鲍菇为试验材料,分别采用0.03 mm的聚乙烯(PE)薄膜袋封口包装、0.05 mm的PE薄膜袋封口包装和1.5%壳聚糖涂膜3种处理,以0.05 mm PE保鲜袋不封口为对照,于4℃、相对湿度85%—95%的条件下贮藏,通过测定失重率、色差值、硬度、可溶性固形物(TSS)、还原糖及可溶性蛋白质含量等理化指标,以及过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)等3种抗氧化酶活性以及相对电导率、丙二醛(MDA)、超氧阴离子()、过氧化氢(H2O2)的含量,研究不同采后处理方式对杏鲍菇贮藏品质及抗氧化酶系统的影响。【结果】与PE不封口处理相比,两种厚度的PE保鲜袋处理能显著降低杏鲍菇子实体失重率(P<0.01),在贮藏18 d时,两种处理的失重率仅为0.35%和0.20%;两种PE处理的菇体硬度显著高于对照(P<0.05),且在贮藏期间两种处理均分别在第3天和第12天出现了两个硬度峰值;PE处理不易发生褐变,但在贮藏末期,0.03 mm PE处理的褐变程度显著增加(P<0.05)。两种PE处理均能显著抑制贮藏期间TSS和可溶性蛋白的增加,减缓还原糖的消耗。1.5%壳聚糖涂膜处理在0—9 d菇体硬度显著增加(P<0.05),随后硬度快速下降;其失重率较高,与对照无显著差异(P>0.05);贮藏期间褐变指数低,但在贮藏末期的褐变程度显著(P<0.05);贮藏的中期与后期,TSS、还原糖和可溶性蛋白含量的变化均显著低于对照。3种处理在一定程度上提高了杏鲍菇的抗氧化能力,与对照处理相比,3种处理均能显著降低膜透性和脂膜过氧化产物MDA的含量(P<0.05),但在贮藏中、后期,壳聚糖处理的电导率和MDA含量均显著高于两种PE处理。0.03 mm PE和1.5%壳聚糖涂膜处理能显著降低超氧阴离子产生速率和过氧化氢�
基金National Natural Science Foundation of China (30470337)Knowledge Innovation Project of Chinese Academy of Sciences (KSCX1-SW-13-03)K C Wong Fellowship, Royal Society of UK.
文摘The effect of thermal stress on the antioxidant system was investigated in two invasive plants, Eupatorium adenophorum Spreng. and E. odoratum L. The former is sensitive to high temperature, whereas the latter is sensitive to low temperature. Our aim was to explore the relationship between the response of antioxidant enzymes and temperature in the two invasive weeds with different distribution patterns in China. Plants were transferred from glasshouse to growth chambers at a constant 25 ℃for I week to acclimatize to the environment. For the heat treatments, temperature was increased stepwise to 30, 35, 38 and finally to 42 ℃. For the cold treatments, temperature was decreased stepwise to 20, 15, 10 and finally to 5 ℃. Plants were kept in the growth chambers for 24 h at each temperature step. In E. adenophorum, the coordinated increase of the activities of antioxidant enzymes was effective in protecting the plant from the accumulation of active oxygen species （AOS） at low temperature, but the activities of catalase （CAT）, guaiacol peroxidase （POD）, ascorbate peroxidase （APX）, glutathione reductase （GR）, and monodehydroascorbate reductase （MDAR） were not accompanied by the increase of superoxide dismutase （SOD） during the heat treatments. As a result, the level of lipid peroxidation in E. adenophorum was higher under heat stress than under cold stress. In E. odoratum, however, the lesser degree of membrane damage, as indicated by low monodehydroascorbate content, and the coordinated increase of the oxygen. Datoxifying enzymes were observed in heat-treated plants, but the antioxidant enzymes were unable to operate in cold stress. This indicates that the plants have a higher capacity for scavenging oxygen radicals in heat stress than in cold stress. The different responses of antioxidant enzymes may be one of the possible mechanisms of the differences in temperature sensitivities of the two plant species.
