Introduction: Prolonged immuno-suppressed status promised to induce internal growth of malignant cell and infectious agent, yet, only a small part of affected individuals seek medical attention or berried by commercia...Introduction: Prolonged immuno-suppressed status promised to induce internal growth of malignant cell and infectious agent, yet, only a small part of affected individuals seek medical attention or berried by commercially over-flowed fake information. Several studies have described complementary and alternative medicine as effective strategies for improving anti-infectious agent including malignant cell. The purpose of this study was to investigate the effect of a fermented herbal decoction (FHD) both in vitro and in vivo to malignant cells and microorganism by regulating leukocyte subset proportioning FHD as dietary material. Methods: In this approach of alternative study, selective anti-cancer effect by fermented decoction was tried to show first in vitro system both, cancer cell and virus strain. The fermented herbal decoction consisting of 80 sorts of herbs and fruits. The selective toxicity was set up and then for immunological factors in animal and human. The most important factor is to reduce side effect for a normal cell. Results: First, FHD was proved as safe by animal test. FHD regulated also the proportion of granulocyte and lymphocyte ratio both animal and human. In vitro culture showed selective toxicity by FHD against human melanoma and leukemia cell line but reduced toxicity was showed by normal cell line. As for the anti-virus activity, anti-virus effect was tested on the feeder layer of human fibroblast cell, after 9 days of culture. Second, FHD inhibits colon cancer growth in 3-methylholanthrene induced cancer in rat. Conclusion: The present results suggest that our fermented herbal decoction showed selective anti-cancer activities and anti-virus activities, together with the regulative effect on the immune system.展开更多
Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to ident...Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to identify novel antiviral therapies.Our previous studies have found that Cryptoporus volvatus extract could potently inhibit influenza virus replication in vitro and in vivo.However,the effective component of Cryptoporus volvatus which mediated the antiviral activity hasn′t been identified.Here,we identified a novel anti-influenza molecule,cryptoporic acid E(CAE),from Cryptoporus volvatus.Our results showed that CAE had broad-spectrum anti-influenza activity against 2009 pandemic strain A/Beijing/07/2009(H1N1/09),seasonal strain A/Jiangxi/262/05(H3N2),mouse adapted strains A/WSN/33(H1N1)and A/PR8/34(H1N1).We further investigated the mode of CAE action,and found that CAE directlyattenuated influenza virus infectivity.Time-course-analysis indicated that CAE exerted its inhibition mainly at middle stage of the replication cycle of influenza virus.Subsequently,we confirmed that CAE blocked virus RNA replication and transcription in MDCK cells and CAE repressed influenza virus RNA polymerase activity.In addition,we found that CAE impaired influenza virus infectivity by directly targeting virus particles.Our data suggest that CAE is a major effective component of Cryptoporus volvatus and might be a potential candidate for the development of a new anti-influenza virus therapy.展开更多
文摘Introduction: Prolonged immuno-suppressed status promised to induce internal growth of malignant cell and infectious agent, yet, only a small part of affected individuals seek medical attention or berried by commercially over-flowed fake information. Several studies have described complementary and alternative medicine as effective strategies for improving anti-infectious agent including malignant cell. The purpose of this study was to investigate the effect of a fermented herbal decoction (FHD) both in vitro and in vivo to malignant cells and microorganism by regulating leukocyte subset proportioning FHD as dietary material. Methods: In this approach of alternative study, selective anti-cancer effect by fermented decoction was tried to show first in vitro system both, cancer cell and virus strain. The fermented herbal decoction consisting of 80 sorts of herbs and fruits. The selective toxicity was set up and then for immunological factors in animal and human. The most important factor is to reduce side effect for a normal cell. Results: First, FHD was proved as safe by animal test. FHD regulated also the proportion of granulocyte and lymphocyte ratio both animal and human. In vitro culture showed selective toxicity by FHD against human melanoma and leukemia cell line but reduced toxicity was showed by normal cell line. As for the anti-virus activity, anti-virus effect was tested on the feeder layer of human fibroblast cell, after 9 days of culture. Second, FHD inhibits colon cancer growth in 3-methylholanthrene induced cancer in rat. Conclusion: The present results suggest that our fermented herbal decoction showed selective anti-cancer activities and anti-virus activities, together with the regulative effect on the immune system.
基金The project supported by Young Scientist Funding from Beijing Natural Science Foundation(7154225)by Innovative Research Team in IMPLAD
文摘Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to identify novel antiviral therapies.Our previous studies have found that Cryptoporus volvatus extract could potently inhibit influenza virus replication in vitro and in vivo.However,the effective component of Cryptoporus volvatus which mediated the antiviral activity hasn′t been identified.Here,we identified a novel anti-influenza molecule,cryptoporic acid E(CAE),from Cryptoporus volvatus.Our results showed that CAE had broad-spectrum anti-influenza activity against 2009 pandemic strain A/Beijing/07/2009(H1N1/09),seasonal strain A/Jiangxi/262/05(H3N2),mouse adapted strains A/WSN/33(H1N1)and A/PR8/34(H1N1).We further investigated the mode of CAE action,and found that CAE directlyattenuated influenza virus infectivity.Time-course-analysis indicated that CAE exerted its inhibition mainly at middle stage of the replication cycle of influenza virus.Subsequently,we confirmed that CAE blocked virus RNA replication and transcription in MDCK cells and CAE repressed influenza virus RNA polymerase activity.In addition,we found that CAE impaired influenza virus infectivity by directly targeting virus particles.Our data suggest that CAE is a major effective component of Cryptoporus volvatus and might be a potential candidate for the development of a new anti-influenza virus therapy.
